FastQCFastQC Report
Mon 3 Jul 2023
SRR5660306.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5660306.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5926442
Total Bases129.8 Mbp
Sequences flagged as poor quality0
Sequence length15-44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCACGTTCCCGTGG2821374.760647282129817No Hit
TTGTCGCTAAGATTCGA919091.5508293171518426No Hit
ACCGGTAGACTTGAAC799761.3494774773801888No Hit
ACCACGTTCCCGTGG789421.3320302468158804No Hit
GGTGAGGGACGACGATT764241.2895426969503794No Hit
GGTGAGGGACGACGATC367320.6197985232961024No Hit
GGTGAGGGACGACGATTT319310.5387887032388067No Hit
CCGGTAGACTTGAAC223000.37627973073894927No Hit
AGACCGGTAGACTTGAAC179930.3036054347617002No Hit
GGTGAGGGACGACGAT161380.27230503563520914No Hit
GCTCGACGCCAGGAT135890.22929440632338932No Hit
GGGTCGAATCTTAGCGAC107000.18054677663259No Hit
GGTGAGGGACGACGATTC106580.17983808835048076No Hit
GACGATTTGTGACACC101020.17045640537779666No Hit
TTTGTCGCTAAGATTCGA95380.16093973416090127No Hit
TGTCGCTAAGATTCGA87360.14740716267872023No Hit
GACCGGTAGACTTGAAC86540.14602353317555458No Hit
AAGATGAGTGCTCTCCT69240.11683232536486479No Hit
GCGACGGACCGGGCC67800.1144025369690617No Hit
GCCCGTCTAGCTCAG65450.11043725729535528No Hit
CGGGGGACGGACTGGGAA63210.10665758645743938No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)