FastQCFastQC Report
Mon 3 Jul 2023
SRR5660307.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5660307.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5468463
Total Bases114.1 Mbp
Sequences flagged as poor quality0
Sequence length15-44
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCACGTTCCCGTGG3777116.9070779120202515No Hit
ACCACGTTCCCGTGG1220352.2316142579734013No Hit
TTGTCGCTAAGATTCGA740941.3549328211601688No Hit
ACCGGTAGACTTGAAC735851.3456249041092534No Hit
GGTGAGGGACGACGATT608391.1125429576829906No Hit
CCGGTAGACTTGAAC315850.5775845973539548No Hit
GGTGAGGGACGACGATC211900.38749462143201846No Hit
GCTCGACGCCAGGAT148720.27195941528725714No Hit
GACCGGTAGACTTGAAC145040.26522991926616307No Hit
GGTGAGGGACGACGAT119260.21808687377056404No Hit
AAGATGAGTGCTCTCCT110060.20126313371782895No Hit
TCCGTTGTAGTCTAGC105460.19285126369146138No Hit
AGATGAGTGCTCTCCT93680.17130956175437229No Hit
TGTCGCTAAGATTCGA78710.14393441082073702No Hit
GATGAGTGCTCTCCT71950.1315726192167708No Hit
GGGGGACGGACTGGGAA59180.10822053655661562No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)