FastQCFastQC Report
Tue 4 Jul 2023
SRR5667267_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5667267_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46651854
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length20-76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAACCCTCAACCATATGTAGTCCAAGCA43460399.315897713304171No Hit
CTTAAAAATTTTAGCAAGAAAGTTCCACAATTGG19817514.24795764815692No Hit
CATGTTATTGTTTCCCAATCAAGCCCGACTAT16399963.5153929788085168No Hit
ACAACCCTCAACCATATGTAGTCCAAGCA10738802.3019020851775793No Hit
CAAGTATTTAATCACATATAAGAACTCCACCGGTAATA9282771.9897965898632883No Hit
CATACGATCTGCATGTTATCTAGAGTTCAACCAATAT9225061.977426234764432No Hit
CTAAATAATCAAGTTCGGTCAACTTTTGCGAAA8272971.7733421698524563No Hit
TACAACCCTCAACCATATGTAGTCCAAGCAG8182151.7538745619841818No Hit
CATACGATCTGCATGTTATCTAGAGTTCAACCAATATAA8005641.7160389810016983No Hit
CATATAAGAACTCCACCGGTAATACGCTTA6593711.413386486204814No Hit
CAGTTCACAATCCCAAGCATGAAAGTGGTTCAG5990121.28400470429321No Hit
CATACGATCTGCATGTTATCTAGAGTTCAACCAAT2762780.5922122623465297No Hit
TACAACCCTAAACCATATGTAGTCCAAGCA2304390.49395464540380324No Hit
GCAACCCTCAACCATATGTAGTCCAAGCA2291760.4912473575005186No Hit
TACAACCCTAACCATATGTAGTCCAAGCA2198740.47130817137513975No Hit
CAAGTATTGTGCCATTGGTCCGTACCTGCGGTT2026010.4342828475798625No Hit
ACAACCCTCAACCATATGTAGTCCAAGCAG1995150.42766789075520983No Hit
TAAAAATTTTAGCAAGAAAGTTCCACAATTGG1878910.4027514104798493No Hit
CATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAA1816800.3894378988667846No Hit
CGATTATATAACAAACTCGTGATACTTTGATCAAGAAG1648170.353291425459747No Hit
AGTATTTAATCACATATAAGAACTCCACCGGTAATA1642250.3520224512406302No Hit
CAACCCTCAACCATATGTAGTCCAAGCA1620940.3474545727593163No Hit
CAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAAT1620920.3474502856842517No Hit
CAGCATGGACTGCGATATGCGTTCAAAATGTCGATGTT1534240.3288701023543459No Hit
TACAACCCTGAACCATATGTAGTCCAAGCA1454390.3117539551589954No Hit
TACAACCCTCAACCATATGTAGTCCAAG1383990.29666345093166074No Hit
CTTAAAAATTTTAGCAAGAAAGTTCCACAATT1344190.28813217155313914No Hit
AAAAATTTTAGCAAGAAAGTTCCACAATTGG1319870.2829190882746053No Hit
CTTTGTCATTGTATTAAATAATGCTTTTATATGTAACTAG1315260.2819309174722188No Hit
CGCAATTTGTCCATTTAAGAAGCTAGTGTCCTTAT1305120.27975737041447485No Hit
CTAATGCAGGTTTTTAAATAGGAGGAACATATGAAAAAATA1251360.26823371264087387No Hit
AAATAATCAAGTTCGGTCAACTTTTGCGAAA956600.20505080033903902No Hit
TTCTTAAAAATTTTAGCAAGAAAGTTCCACAATTGG870890.18667854014976554No Hit
CAAGTCAGCATTTGCCCTTTTGCTCTATGTGTGGTTT869030.18627984216875926No Hit
CGTCAGAACTGTTTCGGTCTTCCATCAGGGTTT846620.1814761745588932No Hit
ACCATATGTAGTCCAAGCAG834020.17877531726820547No Hit
TACAACCCTCAACCATATGTAGTCCAAGCAGATCGTCGGACTGTAGAACT821520.17609589535284065No Hit
CGATCGCTTGGTTTTAGCCTAATAAAAGCACATGT816770.17507771502500202No Hit
TACAACCCTCAACCATATGT790460.16943806777754214No Hit
CATGTTATTGTTTCCCAATCAAGCCCGACTATCT789000.1691251112978275No Hit
CCGACTTCCCTTACCTACATTATTCTATCGACTAGAGA787980.16890647046953375No Hit
CAAACTCCCTACCTGGCAATGTCCTTGAATTGGAT773310.16576190090966159No Hit
CGATCGCTTGGTTTTAGCCTAATAAAAGCACATGTCCCAT763200.16359478446451453No Hit
GCATGTTATCTAGAGTTCAACCAATATAA742970.15925840803668811No Hit
TGAGGGTTGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG699210.14987828779537896TruSeq Adapter, Index 2 (100% over 38bp)
CAACCATATGTAGTCCAAGCA687460.147359631194936No Hit
AATCACATATAAGAACTCCACCGGTAATA659760.14142203223048755No Hit
TCTTAAAAATTTTAGCAAGAAAGTTCCACAATTGG629550.13494640534543387No Hit
AATGCAGGTTTTTAAATAGGAGGAACATATGAAAAAATA599550.1285157927485583No Hit
CTTGTTAAAGGATTTAAAGTGTACTCATTCCAATTACAGGG591130.12671093414636855No Hit
CAGGATGGAGCCACCGATCCAGACGGAGTATTTG562190.1205075365279159No Hit
TTTAGCAAGAAAGTTCCACAATTGG561590.1203789242759784No Hit
ATTCTTAAAAATTTTAGCAAGAAAGTTCCACAATTGG547760.11741441186881875No Hit
GATCACGTCCGGAGACCTCACTAAATAATT527480.11306731775333088No Hit
CAGCTGCTCAACCACTTACAACACCTTGCCTGTT526500.11285725107516628No Hit
CTTGGACTGGGCCTCATCACCGACGTACGAGT501760.10755413922027622No Hit
CGCAATTTGTCCATTTAAGAAGCTAGTGTCCTTATAAT490130.10506120507022079No Hit
TACAACCCTCAACCATATGTAGTCCAAGCT486830.10435383768456448No Hit
ATGTTATTGTTTCCCAATCAAGCCCGACTAT473250.10144291371571215No Hit
TATTCTTAAAAATTTTAGCAAGAAAGTTCCACAATTGG470000.10074626401771727No Hit
TTTTAGCAAGAAAGTTCCACAATTGG468450.10041401570021205No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)