FastQCFastQC Report
Fri 7 Jul 2023
SRR5667268_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5667268_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122734505
Total Bases4.2 Gbp
Sequences flagged as poor quality0
Sequence length20-76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAACCCTCAACCATATGTAGTCCAAGCA99244318.086096896712135No Hit
CTTAAAAATTTTAGCAAGAAAGTTCCACAATTGG69868335.692639571895451No Hit
CATGTTATTGTTTCCCAATCAAGCCCGACTAT31867292.596440992693945No Hit
CATACGATCTGCATGTTATCTAGAGTTCAACCAATAT26989262.1989953029101312No Hit
CTAAATAATCAAGTTCGGTCAACTTTTGCGAAA25306912.0619230101592048No Hit
TACAACCCTCAACCATATGTAGTCCAAGCAG23034151.8767460707158106No Hit
CAAGTATTTAATCACATATAAGAACTCCACCGGTAATA19701861.6052421444157046No Hit
ACAACCCTCAACCATATGTAGTCCAAGCA12141060.9892132615844257No Hit
CAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAAT11840840.9647523326875357No Hit
CATACGATCTGCATGTTATCTAGAGTTCAACCAATATAA11554450.9414182262763027No Hit
CATATAAGAACTCCACCGGTAATACGCTTA10223530.8329792832097217No Hit
CAGTTCACAATCCCAAGCATGAAAGTGGTTCAG9670420.7879137166846437No Hit
TACAACCCTAAACCATATGTAGTCCAAGCA9584570.7809189436988401No Hit
CTAATGCAGGTTTTTAAATAGGAGGAACATATGAAAAAATA8888070.724170436015528No Hit
CATACGATCTGCATGTTATCTAGAGTTCAACCAAT8885590.7239683738489026No Hit
CTTTGTCATTGTATTAAATAATGCTTTTATATGTAACTAG8746060.7125999326758192No Hit
TACAACCCTAACCATATGTAGTCCAAGCA8427240.6866235375292384No Hit
CGATCGCTTGGTTTTAGCCTAATAAAAGCACATGTCCCAT8409490.685177326457625No Hit
AAAAATTTTAGCAAGAAAGTTCCACAATTGG8406070.6848986762117141No Hit
CGATTATATAACAAACTCGTGATACTTTGATCAAGAAG7571550.616904757142256No Hit
TAAAAATTTTAGCAAGAAAGTTCCACAATTGG7426720.6051044895646909No Hit
CTTAAAAATTTTAGCAAGAAAGTTCCACAATT6417730.5228953341197734No Hit
CAAGTATTGTGCCATTGGTCCGTACCTGCGGTT6231770.5077439306900696No Hit
TACAACCCTGAACCATATGTAGTCCAAGCA6051240.4930349456332594No Hit
ACCAAACCGAGGTCTAATATCTCCCACTTATT6031370.4914160039998532No Hit
AATGCAGGTTTTTAAATAGGAGGAACATATGAAAAAATA5609120.45701247583147053No Hit
CTTGTTAAAGGATTTAAAGTGTACTCATTCCAATTACAGGG5460620.4449131888379718No Hit
CAGCATGGACTGCGATATGCGTTCAAAATGTCGATGTT5274160.4297210470682226No Hit
TATTCTTAAAAATTTTAGCAAGAAAGTTCCACAATTGG4699030.3828613640475431No Hit
TCTTAAAAATTTTAGCAAGAAAGTTCCACAATTGG4658120.3795281530650244No Hit
CATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAA4641320.37815934483949726No Hit
CGATCGCTTGGTTTTAGCCTAATAAAAGCACATGT4184970.34097746188001493No Hit
CGGTGGTGGTGAAAGAGTAGCCGCGCTCGGTCAGGAT4036910.32891402462575625No Hit
CTTCATCGTCACACATTTTGTTTTAGTTTTGTTTTTTTTA3955450.3222769342655515No Hit
CGCAATTTGTCCATTTAAGAAGCTAGTGTCCTTAT3858190.3143525123599105No Hit
AGTATTTAATCACATATAAGAACTCCACCGGTAATA3601070.29340322837493826No Hit
CTTATTTATCATTGCAGTCCAGCACGGATACGACCTTAGAGG3575640.29133127640022666No Hit
TTCTTAAAAATTTTAGCAAGAAAGTTCCACAATTGG3466090.2824055060962685No Hit
ATTTAATCACATATAAGAACTCCACCGGTAATACGCTTA3186180.25959936857202465No Hit
CAAGTCAGCATTTGCCCTTTTGCTCTATGTGTGGTTT3123600.2545005579319361No Hit
ATTCTTAAAAATTTTAGCAAGAAAGTTCCACAATTGG3054430.2488648159700485No Hit
AAATAATCAAGTTCGGTCAACTTTTGCGAAA2963680.2414708072517993No Hit
CAGAAGGTCAGGAAACCGAGACGGTCGTCGTGG2779430.22645872894505092No Hit
CGATCGCTTGGTTTTAGCCTAATAAAAGCACATGTCC2757120.22464098421222295No Hit
GCAACCCTCAACCATATGTAGTCCAAGCA2745720.22371215005918668No Hit
ACAACCCTCAACCATATGTAGTCCAAGCAG2709200.22073662170226704No Hit
AATCACATATAAGAACTCCACCGGTAATA2585630.21066854834343446No Hit
TTTAGCAAGAAAGTTCCACAATTGG2462970.20067461876348464No Hit
CATGTTATTGTTTCCCAATCAAGCCCGACTATCT2165390.17642878830203454No Hit
CAAACTCCCTACCTGGCAATGTCCTTGAATTGGAT2158760.17588859791303188No Hit
GCATGTTATCTAGAGTTCAACCAATATAA2029150.1653284054064503No Hit
CACATATAAGAACTCCACCGGTAATACGCTTACATA2015870.16424639509484312No Hit
TTTTAGCAAGAAAGTTCCACAATTGG2014990.16417469561636314No Hit
CATATAAATCTATCAGCACTTTATCAAATTAATAA2001120.16304461406350237No Hit
TAATGCAGGTTTTTAAATAGGAGGAACATATGAAAAAATA1976220.16101584472923894No Hit
AGTATTCTTAAAAATTTTAGCAAGAAAGTTCCACAATTGG1961680.15983117380071726No Hit
TGAGGGTTGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCT1936860.1578089226008611TruSeq Adapter, Index 10 (100% over 38bp)
CTTGGACTGGGCCTCATCACCGACGTACGAGT1837920.1497476198726674No Hit
CAAAGCGAGAATCATACCCCTAGACCATTGAGCC1820680.1483429619079003No Hit
CATGTAGTTCACATCGATGAAGGGATCGTTGA1809090.14739864718564677No Hit
AGAAACTCTATTTACCCAGAACGAGCACATAAACCAT1807640.14728050599951498No Hit
CTAGAAACTCTATTTACCCAGAACGAGCACATAAACCAT1777910.14485820430041252No Hit
TACAACCCTCAACCATATGT1764520.14376723155399534No Hit
CGCAATTTGTCCATTTAAGAAGCTAGTGTCCTTATAAT1689810.1376801087844042No Hit
CAGGATGGAGCCACCGATCCAGACGGAGTATTTG1619050.13191481890117207No Hit
ACCATATGTAGTCCAAGCAG1588070.12939067135195598No Hit
GCCAAACCGAGGTCTAATATCTCCCACTTATT1554680.12667016500372083No Hit
TACAACCCTCAACCATATGTAGTCCAAG1540190.1254895679092037No Hit
TACAACCCTAAACCATATGTAGTCCAAGCAG1531510.12478235032601467No Hit
GCATGTTATCTAGAGTTCAACCAATAT1500570.12226146184400222No Hit
TACAACCCTAACCATATGTAGTCCAAGCAG1479200.12052030519045968No Hit
GATCACGTCCGGAGACCTCACTAAATAATT1450460.11817866540464722No Hit
CGTCAGAACTGTTTCGGTCTTCCATCAGGGTTT1416250.1153913481787375No Hit
TACAACCCTCAACCATATGTAGTCCTAGCA1329290.10830613607803283No Hit
CGATGGTGATCACCTGTCCGTCGGGAAGCTCGTA1319460.10750522031273926No Hit
CAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTT1314480.1070994664458866No Hit
CTTAGAAGCACTTGCGGTGGACGATGCCAGGG1286590.10482708183815138No Hit
CAGGGAGGTGATCTCCTTCTGCATACGATCGGCAATA1271690.10361307930479696No Hit
AATTTTAGCAAGAAAGTTCCACAATTGG1252120.10201858067541804No Hit
CAACCATATGTAGTCCAAGCA1242480.10123314547934177No Hit
CGTCACAGTGATCACGTCCGGAGACCTCACTAAATAATT1234660.10059599784103092No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)