FastQCFastQC Report
Mon 3 Jul 2023
SRR5667269_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5667269_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45156367
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length20-76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAACCCTCAACCATATGTAGTCCAAGCA39458008.738081165829838No Hit
CTTAAAAATTTTAGCAAGAAAGTTCCACAATTGG26301755.8245939049968305No Hit
CAAGTATTTAATCACATATAAGAACTCCACCGGTAATA13847143.066486726002559No Hit
CATGTTATTGTTTCCCAATCAAGCCCGACTAT12693612.811034377499855No Hit
CTAAATAATCAAGTTCGGTCAACTTTTGCGAAA8462391.8740192274546799No Hit
TACAACCCTCAACCATATGTAGTCCAAGCAG8029061.7780571231516478No Hit
CATACGATCTGCATGTTATCTAGAGTTCAACCAATAT6618001.4655740573638265No Hit
CATATAAGAACTCCACCGGTAATACGCTTA6268931.3882715586929302No Hit
TACAACCCTAAACCATATGTAGTCCAAGCA5297881.1732299013337366No Hit
CAGTTCACAATCCCAAGCATGAAAGTGGTTCAG4803471.0637414652954698No Hit
CATACGATCTGCATGTTATCTAGAGTTCAACCAATATAA4724911.046344140129785No Hit
TACAACCCTAACCATATGTAGTCCAAGCA4723411.0460119610596663No Hit
AAAAATTTTAGCAAGAAAGTTCCACAATTGG4016840.8895401173438067No Hit
CTAATGCAGGTTTTTAAATAGGAGGAACATATGAAAAAATA3936960.8718504745964174No Hit
CGATCGCTTGGTTTTAGCCTAATAAAAGCACATGTCCCAT3739860.8282021447828166No Hit
TACAACCCTGAACCATATGTAGTCCAAGCA3501690.775458752029365No Hit
CAAGTATTGTGCCATTGGTCCGTACCTGCGGTT3416800.7566596311877791No Hit
CTTTGTCATTGTATTAAATAATGCTTTTATATGTAACTAG3367840.7458173063391038No Hit
CGATTATATAACAAACTCGTGATACTTTGATCAAGAAG2971220.657984731145444No Hit
TAAAAATTTTAGCAAGAAAGTTCCACAATTGG2864750.6344066607484167No Hit
TATTCTTAAAAATTTTAGCAAGAAAGTTCCACAATTGG2819550.6243969981021724No Hit
ACAACCCTCAACCATATGTAGTCCAAGCA2614930.5790833438837097No Hit
CATACGATCTGCATGTTATCTAGAGTTCAACCAAT2143560.4746971783624666No Hit
CTTGTTAAAGGATTTAAAGTGTACTCATTCCAATTACAGGG2057200.45557252203216436No Hit
CAGCATGGACTGCGATATGCGTTCAAAATGTCGATGTT2008040.4446859066408066No Hit
TCTTAAAAATTTTAGCAAGAAAGTTCCACAATTGG1974270.437207448508867No Hit
CATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAA1785860.3954835427748207No Hit
CAAGTCAGCATTTGCCCTTTTGCTCTATGTGTGGTTT1748990.3873185812313023No Hit
AGTATTTAATCACATATAAGAACTCCACCGGTAATA1684340.37300166330918516No Hit
CAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAAT1680230.3720914926570599No Hit
CGGTGGTGGTGAAAGAGTAGCCGCGCTCGGTCAGGAT1631690.3613421779480178No Hit
CGATCGCTTGGTTTTAGCCTAATAAAAGCACATGTCC1520130.336636913239721No Hit
CTTAAAAATTTTAGCAAGAAAGTTCCACAATT1514900.33547871554857367No Hit
AATGCAGGTTTTTAAATAGGAGGAACATATGAAAAAATA1388420.30746937635616256No Hit
ACCAAACCGAGGTCTAATATCTCCCACTTATT1371970.3038264792205272No Hit
TACAACCCTCAACCATATGTAGTCCAAG1371210.3036581751583337No Hit
CGCAATTTGTCCATTTAAGAAGCTAGTGTCCTTAT1297560.28734818281550417No Hit
CTTCATCGTCACACATTTTGTTTTAGTTTTGTTTTTTTTA1266630.280498650389656No Hit
CTTAAATTCAGGGGGTAGTCCCATATGAGTTGAGGTTGT1222170.27065286275133693No Hit
TTCTTAAAAATTTTAGCAAGAAAGTTCCACAATTGG1164880.2579658367999357No Hit
TTTAGCAAGAAAGTTCCACAATTGG1083960.24004588323059736No Hit
CGATCGCTTGGTTTTAGCCTAATAAAAGCACATGT1069230.23678388476203144No Hit
TTTTAGCAAGAAAGTTCCACAATTGG939170.20798174485560364No Hit
CATGTTATTGTTTCCCAATCAAGCCCGACTATCT929230.20578050488428354No Hit
CAAACTCCCTACCTGGCAATGTCCTTGAATTGGAT875360.19385084721275295No Hit
CAGAAGGTCAGGAAACCGAGACGGTCGTCGTGG858760.19017473217010572No Hit
CATATAAATCTATCAGCACTTTATCAAATTAATAA851110.1884806189125002No Hit
ATTTAATCACATATAAGAACTCCACCGGTAATACGCTTA811670.1797465238955118No Hit
TAATGCAGGTTTTTAAATAGGAGGAACATATGAAAAAATA802010.17760729068394718No Hit
TACAACCCTCAACCATATGT800870.17735483459065696No Hit
TACAACCCTAAACCATATGTAGTCCAAGCAG748600.16577950126058635No Hit
ATTCTTAAAAATTTTAGCAAGAAAGTTCCACAATTGG743190.16458144208102482No Hit
GATCACGTCCGGAGACCTCACTAAATAATT722890.16008595199875136No Hit
TACAACCCTAACCATATGTAGTCCAAGCAG709370.15709191131341455No Hit
CGTCAGAACTGTTTCGGTCTTCCATCAGGGTTT686410.1520073570134639No Hit
CAACCATATGTAGTCCAAGCA684120.1515002302997493No Hit
CCGACTTCCCTTACCTACATTATTCTATCGACTAGAGA662260.1466592739845524No Hit
AAATAATCAAGTTCGGTCAACTTTTGCGAAA634550.14052281929589244No Hit
TACAACCCTGACCATATGTAGTCCAAGCA618710.13701500831543867No Hit
CGTTGATACTTTCATTGTAGCGCGCGTGCAGCCCAGGA611450.13540726161606403No Hit
CGCAATTTGTCCATTTAAGAAGCTAGTGTCCTTATAAT609460.13496657071637316No Hit
CAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTT604580.13388588147492025No Hit
CACATATAAGAACTCCACCGGTAATACGCTTACATA576970.12777157205760153No Hit
GCATGTTATCTAGAGTTCAACCAATATAA559350.12386957524727356No Hit
AGTATTCTTAAAAATTTTAGCAAGAAAGTTCCACAATTGG552710.12239912923021465No Hit
ACAACCCTCAACCATATGTAGTCCAAGCAG543070.12026432507291829No Hit
CATGTAGTTCACATCGATGAAGGGATCGTTGA533650.11817824051257267No Hit
AATCACATATAAGAACTCCACCGGTAATA531450.11769104454306523No Hit
CTAGAAACTCTATTTACCCAGAACGAGCACATAAACCAT523320.1158906339830217No Hit
CTGGAAGGTGGACAGAGAGGCCAGGATGGAG501550.1110696084120319No Hit
CGTCACAGTGATCACGTCCGGAGACCTCACTAAATAATT500560.11085037022575353No Hit
TACAACCCTGAACCATATGTAGTCCAAGCAG498040.11029230938795409No Hit
CATAATCCAACGGACGTAGCGTCATACCACTGTTCG498020.11028788033368582No Hit
TACAACCCTACCATATGTAGTCCAAGCA491140.10876428566540793No Hit
CATGATGGAGTTGTAGACGGTCTCGTGGATG476540.10553107604958564No Hit
CTTAGAAGCACTTGCGGTGGACGATGCCAGGG458650.10156928700663631No Hit
TACAACCCTCAACCATATGTAG457620.10134119071182143No Hit
CAAAGCGAGAATCATACCCCTAGACCATTGAGCC456550.10110423630847007No Hit
CTTGGACTGGGCCTCATCACCGACGTACGAGT456500.10109316367279947No Hit
CAGGATGGAGCCACCGATCCAGACGGAGTATTTG454270.10059932412188961No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)