FastQCFastQC Report
Mon 3 Jul 2023
SRR5667276_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5667276_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35199688
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length20-76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAACCCTCAACCATATGTAGTCCAAGCA545909815.50893860195579No Hit
CTTAAAAATTTTAGCAAGAAAGTTCCACAATTGG16298964.6304274060610995No Hit
CATGTTATTGTTTCCCAATCAAGCCCGACTAT14310034.06538546591663No Hit
ACAACCCTCAACCATATGTAGTCCAAGCA11391873.2363553904227786No Hit
TACAACCCTCAACCATATGTAGTCCAAGCAG10106562.871207267518962No Hit
CATACGATCTGCATGTTATCTAGAGTTCAACCAATATAA6310331.792723276410859No Hit
CATATAAGAACTCCACCGGTAATACGCTTA6011471.7078191147603352No Hit
CATACGATCTGCATGTTATCTAGAGTTCAACCAATAT5947821.6897365681195808No Hit
CAAGTATTTAATCACATATAAGAACTCCACCGGTAATA5875861.6692932050988634No Hit
CAGTTCACAATCCCAAGCATGAAAGTGGTTCAG5598841.5905936438982071No Hit
CTAAATAATCAAGTTCGGTCAACTTTTGCGAAA5280041.5000246593094801No Hit
TACAACCCTAAACCATATGTAGTCCAAGCA4150551.1791439742306806No Hit
TACAACCCTAACCATATGTAGTCCAAGCA3929311.11629114439878No Hit
TACAACCCTGAACCATATGTAGTCCAAGCA2845400.80835943773138No Hit
CAACCCTCAACCATATGTAGTCCAAGCA2460800.6990971056334363No Hit
CAAGTATTGTGCCATTGGTCCGTACCTGCGGTT2171790.6169912642407512No Hit
ACAACCCTCAACCATATGTAGTCCAAGCAG2089580.5936359435913182No Hit
TACAACCCTCAACCATATGTAGTCCAAG1795460.5100783847856833No Hit
CATACGATCTGCATGTTATCTAGAGTTCAACCAAT1608310.45691029988674897No Hit
GCAACCCTCAACCATATGTAGTCCAAGCA1526510.43367145754246456No Hit
TGAGGGTTGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAA1474320.4188446215773276TruSeq Adapter, Index 6 (100% over 38bp)
CGATTATATAACAAACTCGTGATACTTTGATCAAGAAG1424440.40467404142900354No Hit
TAAAAATTTTAGCAAGAAAGTTCCACAATTGG1270900.3610543366179837No Hit
CATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAA1182370.33590354550869883No Hit
CAGCATGGACTGCGATATGCGTTCAAAATGTCGATGTT1112840.31615052951605704No Hit
CTTTGTCATTGTATTAAATAATGCTTTTATATGTAACTAG981680.27888883560558836No Hit
CTTAAAAATTTTAGCAAGAAAGTTCCACAATT859420.24415557319712608No Hit
AAAAATTTTAGCAAGAAAGTTCCACAATTGG794450.22569802323247867No Hit
TACAACCCTCAACCATATGT786550.22345368515766392No Hit
CGCAATTTGTCCATTTAAGAAGCTAGTGTCCTTAT746540.21208710713572235No Hit
CGATCGCTTGGTTTTAGCCTAATAAAAGCACATGT710190.20176031105730252No Hit
AGTATTTAATCACATATAAGAACTCCACCGGTAATA689100.19576878067782874No Hit
CAACCATATGTAGTCCAAGCA672640.19109260286625268No Hit
CAAGTCAGCATTTGCCCTTTTGCTCTATGTGTGGTTT672620.19108692099770883No Hit
CTAATGCAGGTTTTTAAATAGGAGGAACATATGAAAAAATA672420.1910301023122705No Hit
ACCATATGTAGTCCAAGCAG660240.1875698443690751No Hit
CATGTTATTGTTTCCCAATCAAGCCCGACTATCT653730.18572039615805686No Hit
CGTCAGAACTGTTTCGGTCTTCCATCAGGGTTT649580.18454140843521114No Hit
ACAACCCTAAACCATATGTAGTCCAAGCA636720.18088796696152534No Hit
ACAACCCTAACCATATGTAGTCCAAGCA635580.18056410045452675No Hit
CAAACTCCCTACCTGGCAATGTCCTTGAATTGGAT606010.17216345781246697No Hit
TACAACCCTCAACCATATGTAGTCCAAGCT588210.167106594808454No Hit
TACAACCCTCAACCATATGTAGTCCTAGCA569340.16174575183734585No Hit
CAGGATGGAGCCACCGATCCAGACGGAGTATTTG543410.154379209270264No Hit
TACAACCCTAACCATATGTAGTCCAAGCAG524460.1489956388249805No Hit
TACAACCCTAAACCATATGTAGTCCAAGCAG522640.14847858878749154No Hit
TACAACCCTCAACCATATGTAGTCCAAGCAGATCGTCGGACTGTAGAACT516430.1467143686046308No Hit
CCGACTTCCCTTACCTACATTATTCTATCGACTAGAGA508500.1444615077270003No Hit
TACAACCCTGACCATATGTAGTCCAAGCA506410.14386775246416958No Hit
TCTTAAAAATTTTAGCAAGAAAGTTCCACAATTGG494650.1405268137603947No Hit
CTTGGACTGGGCCTCATCACCGACGTACGAGT460780.13090456938141043No Hit
CAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAAT458840.13035342813265846No Hit
CACAACCCTCAACCATATGTAGTCCAAGCA455080.12928523684641752No Hit
TTCTTAAAAATTTTAGCAAGAAAGTTCCACAATTGG453190.12874830026902512No Hit
GCATGTTATCTAGAGTTCAACCAATATAA452810.12864034476669223No Hit
TGCTTGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAT452440.1285352301986313TruSeq Adapter, Index 6 (100% over 39bp)
TTTAGCAAGAAAGTTCCACAATTGG440080.1250238354385414No Hit
ACAACCCTGAACCATATGTAGTCCAAGCA438160.12447837605833324No Hit
CTTGTTAAAGGATTTAAAGTGTACTCATTCCAATTACAGGG430210.1222198333121589No Hit
CGATCGCTTGGTTTTAGCCTAATAAAAGCACATGTCCCAT428130.12162891898360008No Hit
TACAACCCTCAACCATATGTAG425140.12077947963629679No Hit
ACAACCCTCAACCATATGTAGTCCAAG412000.1170464920029973No Hit
TACAACCCTCAACCATATGTA409870.11644137300307889No Hit
TGAGGGTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAT402080.11422828520525523TruSeq Adapter, Index 6 (100% over 39bp)
TACAACCCTACCATATGTAGTCCAAGCA398310.11315725298474237No Hit
TACAACCCTCAACCATATGTAGTCCGAGCA396140.11254077024773629No Hit
AAATAATCAAGTTCGGTCAACTTTTGCGAAA387600.11011461237951882No Hit
GATCACGTCCGGAGACCTCACTAAATAATT381740.10844982489617522No Hit
TTTTAGCAAGAAAGTTCCACAATTGG365800.10392137566673887No Hit
TACAACCCTCAACCATATGTAGTCCAAGCG353090.10031054820713184No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)