FastQCFastQC Report
Tue 4 Jul 2023
SRR5667277_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5667277_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78959629
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length20-72
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAACCCTCAACCATATGTAGTCCAAGCA904160111.450916265070092No Hit
CTTAAAAATTTTAGCAAGAAAGTTCCACAATTGG48525576.1456177814614605No Hit
CATGTTATTGTTTCCCAATCAAGCCCGACTAT25307533.2051227089732146No Hit
TACAACCCTCAACCATATGTAGTCCAAGCAG19376372.4539590985160276No Hit
CATACGATCTGCATGTTATCTAGAGTTCAACCAATAT18081132.2899208404335334No Hit
CAAGTATTTAATCACATATAAGAACTCCACCGGTAATA14410091.8249946437818243No Hit
TACAACCCTAAACCATATGTAGTCCAAGCA11358091.438468004959851No Hit
CTAAATAATCAAGTTCGGTCAACTTTTGCGAAA10185821.2900035282587259No Hit
TACAACCCTAACCATATGTAGTCCAAGCA9923141.256735894744389No Hit
CATATAAGAACTCCACCGGTAATACGCTTA9738881.2333999188370046No Hit
ACAACCCTCAACCATATGTAGTCCAAGCA8028561.0167930246987356No Hit
TACAACCCTGAACCATATGTAGTCCAAGCA7075750.8961224982452741No Hit
CAGTTCACAATCCCAAGCATGAAAGTGGTTCAG6540680.8283574888630746No Hit
CATACGATCTGCATGTTATCTAGAGTTCAACCAAT6281980.7955939104019852No Hit
CATACGATCTGCATGTTATCTAGAGTTCAACCAATATAA6207970.7862207660575506No Hit
AAAAATTTTAGCAAGAAAGTTCCACAATTGG5657140.7164597999820896No Hit
CTTTGTCATTGTATTAAATAATGCTTTTATATGTAACTAG5406310.6846929333976481No Hit
CTTAAAAATTTTAGCAAGAAAGTTCCACAATT5223410.6615291974079565No Hit
CGATTATATAACAAACTCGTGATACTTTGATCAAGAAG4910080.6218468934295525No Hit
TAAAAATTTTAGCAAGAAAGTTCCACAATTGG4631010.5865035156130229No Hit
ACCAAACCGAGGTCTAATATCTCCCACTTATT4512460.571489513964155No Hit
CAAGTATTGTGCCATTGGTCCGTACCTGCGGTT4430220.5610740648236835No Hit
CGATCGCTTGGTTTTAGCCTAATAAAAGCACATGTCCCAT4087070.5176151473558722No Hit
CTAATGCAGGTTTTTAAATAGGAGGAACATATGAAAAAATA3868280.4899060505970716No Hit
TATTCTTAAAAATTTTAGCAAGAAAGTTCCACAATTGG3390220.4293611865881487No Hit
TCTTAAAAATTTTAGCAAGAAAGTTCCACAATTGG3350120.4242826419561824No Hit
CGATCGCTTGGTTTTAGCCTAATAAAAGCACATGT3054360.3868255257379692No Hit
CAGCATGGACTGCGATATGCGTTCAAAATGTCGATGTT3041560.3852044441596857No Hit
CATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAA2578340.3265390216055853No Hit
CAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAAT2493240.3157613620499661No Hit
CTTCATCGTCACACATTTTGTTTTAGTTTTGTTTTTTTTA2492100.31561698447190023No Hit
CGCAATTTGTCCATTTAAGAAGCTAGTGTCCTTAT2210830.2799949832591032No Hit
CGGTGGTGGTGAAAGAGTAGCCGCGCTCGGTCAGGAT2180340.27613351628083255No Hit
CTTGTTAAAGGATTTAAAGTGTACTCATTCCAATTACAGGG2150680.2723771663111538No Hit
CAAGTCAGCATTTGCCCTTTTGCTCTATGTGTGGTTT2007930.25429830730334363No Hit
CGATCGCTTGGTTTTAGCCTAATAAAAGCACATGTCC1926310.24396137930182016No Hit
TACAACCCTAAACCATATGTAGTCCAAGCAG1770720.22425637283579433No Hit
AGTATTTAATCACATATAAGAACTCCACCGGTAATA1759230.2228011988252883No Hit
TACAACCCTAACCATATGTAGTCCAAGCAG1729420.21902585180586398No Hit
AATGCAGGTTTTTAAATAGGAGGAACATATGAAAAAATA1721710.21804940344894475No Hit
TTCTTAAAAATTTTAGCAAGAAAGTTCCACAATTGG1696610.21487056379152947No Hit
ACAACCCTCAACCATATGTAGTCCAAGCAG1690230.21406255594235377No Hit
CAGAAGGTCAGGAAACCGAGACGGTCGTCGTGG1680070.21277582243959126No Hit
CATGTTATTGTTTCCCAATCAAGCCCGACTATCT1674640.2120881292388038No Hit
TTTAGCAAGAAAGTTCCACAATTGG1596310.20216786986169857No Hit
TACAACCCTCAACCATATGTAGTCCAAG1574730.19943482763831122No Hit
ATTTAATCACATATAAGAACTCCACCGGTAATACGCTTA1530790.19386995853285988No Hit
TACAACCCTCAACCATATGT1522000.192756731417773No Hit
CACATATAAGAACTCCACCGGTAATACGCTTACATA1520100.19251610212099654No Hit
CAAACTCCCTACCTGGCAATGTCCTTGAATTGGAT1341170.16985515471456938No Hit
TTTTAGCAAGAAAGTTCCACAATTGG1326810.1680365038189326No Hit
TACAACCCTCAACCATATGTAGTCCTAGCA1310000.16590756777745244No Hit
CTTGGACTGGGCCTCATCACCGACGTACGAGT1282710.1624513711937527No Hit
TACAACCCTGACCATATGTAGTCCAAGCA1222080.1547727636866176No Hit
ATTCTTAAAAATTTTAGCAAGAAAGTTCCACAATTGG1186070.15021220527771223No Hit
GCAACCCTCAACCATATGTAGTCCAAGCA1155840.14638366651900048No Hit
CATGTAGTTCACATCGATGAAGGGATCGTTGA1150140.1456617786286711No Hit
TACAACACTCAACCATATGTAGTCCAAGCA1119450.14177498225073978No Hit
AGAAACTCTATTTACCCAGAACGAGCACATAAACCAT1117600.1415406853038785No Hit
CAACCATATGTAGTCCAAGCA1108790.14042492524882558No Hit
TACAACCCTGAACCATATGTAGTCCAAGCAG1100120.1393268957735351No Hit
TACAACCTCAACCATATGTAGTCCAAGCA1040930.13183065994395693No Hit
CTAGAAACTCTATTTACCCAGAACGAGCACATAAACCAT1037710.13142285660942No Hit
CGCAATTTGTCCATTTAAGAAGCTAGTGTCCTTATAAT1012600.12824275048202166No Hit
CATATAAATCTATCAGCACTTTATCAAATTAATAA1011670.12812496877359947No Hit
CAGGATGGAGCCACCGATCCAGACGGAGTATTTG992160.12565408583670015No Hit
AATCACATATAAGAACTCCACCGGTAATA989810.12535646539068718No Hit
TACAACCCTACCATATGTAGTCCAAGCA988830.12523235133234986No Hit
ACCATATGTAGTCCAAGCAG980230.12414318714694063No Hit
GCATGTTATCTAGAGTTCAACCAATATAA927090.117413165657098No Hit
CGATGGTGATCACCTGTCCGTCGGGAAGCTCGTA924350.1170661528817467No Hit
TACAACCCTCAACCATATGTAG923480.11695596999322273No Hit
CGTCAGAACTGTTTCGGTCTTCCATCAGGGTTT914850.11586300639786441No Hit
CTTAGAAGCACTTGCGGTGGACGATGCCAGGG906370.1147890398522516No Hit
GATCACGTCCGGAGACCTCACTAAATAATT875350.11086044996488015No Hit
TACAACCCTCAACCATATGTA869840.11016262500422842No Hit
TAATGCAGGTTTTTAAATAGGAGGAACATATGAAAAAATA869580.11012969678466955No Hit
CGTGATACTTTGATCAAGAAG857940.10865552572441799No Hit
GCATGTTATCTAGAGTTCAACCAATAT845730.10710916587513349No Hit
CAGGGAGGTGATCTCCTTCTGCATACGATCGGCAATA828820.10496756513382301No Hit
CACAACCCTCAACCATATGTAGTCCAAGCA817700.1035592505126892No Hit
GCCAAACCGAGGTCTAATATCTCCCACTTATT809090.10246881985729694No Hit
TACAACCCTCAACCATATGTAGTCCGAGCA807100.10221679233067318No Hit
AGTATTCTTAAAAATTTTAGCAAGAAAGTTCCACAATTGG792820.10040827319490064No Hit
TACAACCCTCAACCATATGTAGTCCAAGCT790730.10014358096844655No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)