FastQCFastQC Report
Mon 3 Jul 2023
SRR5667278_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5667278_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20752434
Total Bases676.5 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAACCCTCAACCATATGTAGTCCAAGCA485991723.418539724063212No Hit
TACAACCCTCAACCATATGTAGTCCAAGCAG8689524.18722931488422No Hit
TACAACCCTAAACCATATGTAGTCCAAGCA8545294.117729033616008No Hit
TACAACCCTAACCATATGTAGTCCAAGCA7721003.720527433071225No Hit
CTTAAAAATTTTAGCAAGAAAGTTCCACAATTGG7311773.5233312873082743No Hit
TACAACCCTGAACCATATGTAGTCCAAGCA5629122.712510734885363No Hit
CATGTTATTGTTTCCCAATCAAGCCCGACTAT3945291.9011215744620606No Hit
ACAACCCTCAACCATATGTAGTCCAAGCA3944291.9006397032752882No Hit
CAGTTCACAATCCCAAGCATGAAAGTGGTTCAG2370711.1423768411936643No Hit
CATACGATCTGCATGTTATCTAGAGTTCAACCAATAT2298521.1075905602205505No Hit
CTAAATAATCAAGTTCGGTCAACTTTTGCGAAA2122741.0228872430096634No Hit
CATATAAGAACTCCACCGGTAATACGCTTA1255870.6051675673321019No Hit
CAAGTATTGTGCCATTGGTCCGTACCTGCGGTT1200220.5783514357882069No Hit
CATACGATCTGCATGTTATCTAGAGTTCAACCAATATAA1110460.5350986780634985No Hit
TACAACCCTAAACCATATGTAGTCCAAGCAG1100440.5302703287720371No Hit
CAAGTATTTAATCACATATAAGAACTCCACCGGTAATA1061650.511578545437128No Hit
TACAACCCTAACCATATGTAGTCCAAGCAG1050770.5063357869250422No Hit
CGATCGCTTGGTTTTAGCCTAATAAAAGCACATGTCCCAT1048310.5051503838055815No Hit
TACAACCCTGACCATATGTAGTCCAAGCA1022530.49272774461058394No Hit
TACAACCCTCAACCATATGTAGTCCAAG919110.442892626474562No Hit
CGATCGCTTGGTTTTAGCCTAATAAAAGCACATGT817900.3941224436613074No Hit
CTAATGCAGGTTTTTAAATAGGAGGAACATATGAAAAAATA795400.3832803419589239No Hit
TACAACCCTACCATATGTAGTCCAAGCA789400.38038911483828836No Hit
AAAAATTTTAGCAAGAAAGTTCCACAATTGG779720.3757246017503296No Hit
CTTAAAAATTTTAGCAAGAAAGTTCCACAATT747810.360348092180416No Hit
CGATTATATAACAAACTCGTGATACTTTGATCAAGAAG736420.35485957936307616No Hit
TACAACCCTGAACCATATGTAGTCCAAGCAG724320.34902893800312773No Hit
CTTTGTCATTGTATTAAATAATGCTTTTATATGTAACTAG686750.3309250375160812No Hit
CATACGATCTGCATGTTATCTAGAGTTCAACCAAT673980.32477154246099516No Hit
ACAACCCTCAACCATATGTAGTCCAAGCAG648600.31254165174070664No Hit
CGCAATTTGTCCATTTAAGAAGCTAGTGTCCTTAT630980.3040510814297735No Hit
TAAAAATTTTAGCAAGAAAGTTCCACAATTGG621390.2994299367486243No Hit
TACAACACTCAACCATATGTAGTCCAAGCA601030.2896190393859342No Hit
CAGCATGGACTGCGATATGCGTTCAAAATGTCGATGTT569330.2743437227652429No Hit
TGAGGGTTGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCA555770.26780954947260643TruSeq Adapter, Index 9 (100% over 38bp)
TACAACCTCAACCATATGTAGTCCAAGCA549620.264846041673955No Hit
ACAACCCTAAACCATATGTAGTCCAAGCA519910.2505296487149411No Hit
ACAACCCTAACCATATGTAGTCCAAGCA512030.24673250376317304No Hit
CTTGGTTTTAGCCTAATAAAAGCACATGTCCCAT506720.24417376776141053No Hit
ACCAAACCGAGGTCTAATATCTCCCACTTATT450980.21731426781070595No Hit
TCTTAAAAATTTTAGCAAGAAAGTTCCACAATTGG444460.21417246767294865No Hit
CACAACCCTCAACCATATGTAGTCCAAGCA415490.2002126593921465No Hit
CAACCCTCAACCATATGTAGTCCAAGCA408920.19704676569505053No Hit
TACAACCCTCAACCATATGT378830.18254726168506305No Hit
CTTGTTAAAGGATTTAAAGTGTACTCATTCCAATTACAGGG373080.17977650236112064No Hit
CGCAATTTGTCCATTTAAGAAGCTAGTGTCCTTATAAT364770.17577215279904035No Hit
ACAACCCTGAACCATATGTAGTCCAAGCA348430.1678983776071761No Hit
CAAGTCAGCATTTGCCCTTTTGCTCTATGTGTGGTTT343810.1656721327242867No Hit
TACAACCCTCAACCATATGTAGTCCAAGCT330650.15933070790635934No Hit
CAACCATATGTAGTCCAAGCA306330.14761160064404977No Hit
TATTCTTAAAAATTTTAGCAAGAAAGTTCCACAATTGG304830.14688879386389087No Hit
CAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAAT296340.1427977074881915No Hit
TACAACCCTCAACCATATGTAGTCCGAGCA291730.14057628131716982No Hit
CATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAA290110.13979564999459823No Hit
GCAACCCTCAACCATATGTAGTCCAAGCA289750.1396221763673601No Hit
TACAACCCTCAACCATATGTAGTCCAAGCG276960.13345904388853855No Hit
TACAACCCTCAACAATATGTAGTCCAAGCA266640.12848613324104535No Hit
TACAACCCTCAACCATATGTAGTCCAGGCA262400.12644299940912954No Hit
TACAACCCTCAACCATATGTAGTCCTAGCA262020.12625988835815596No Hit
TACAACCCTTAACCATATGTAGTCCAAGCA259800.1251901343235208No Hit
ACCATATGTAGTCCAAGCAG241970.11659837106336539No Hit
TTCTTAAAAATTTTAGCAAGAAAGTTCCACAATTGG231500.11155317973785628No Hit
CATGTTATTGTTTCCCAATCAAGCCCGACTATCT228620.11016539071995121No Hit
TACAACCCTCAACCATATGTAGTCAAGCA226630.10920646705827373No Hit
AATGCAGGTTTTTAAATAGGAGGAACATATGAAAAAATA224890.10836801119328943No Hit
CAGAAGGTCAGGAAACCGAGACGGTCGTCGTGG222410.10717297065009339No Hit
TACAACGCTCAACCATATGTAGTCCAAGCA215060.10363121742731479No Hit
CACATATAAGAACTCCACCGGTAATACGCTTACATA210340.10135678542574814No Hit
TACAACCATCAACCATATGTAGTCCAAGCA209950.10116885566290681No Hit
CAAACTCCCTACCTGGCAATGTCCTTGAATTGGAT209830.1011110311204941No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)