FastQCFastQC Report
Tue 4 Jul 2023
SRR5667282_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5667282_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56343352
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length20-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAACCCTCAACCATATGTAGTCCAAGCA53681059.527486046623567No Hit
CATGTTATTGTTTCCCAATCAAGCCCGACTAT18405213.2666160863130753No Hit
CTTAAAAATTTTAGCAAGAAAGTTCCACAATTGG16268902.887456891098705No Hit
CTAAATAATCAAGTTCGGTCAACTTTTGCGAAA16026172.8443763871201697No Hit
CAAGTATTTAATCACATATAAGAACTCCACCGGTAATA14113902.504980534349465No Hit
CATATAAGAACTCCACCGGTAATACGCTTA10149271.8013252033709317No Hit
TACAACCCTCAACCATATGTAGTCCAAGCAG10114431.7951416877007957No Hit
ACAACCCTCAACCATATGTAGTCCAAGCA8723501.5482749411146146No Hit
CATACGATCTGCATGTTATCTAGAGTTCAACCAATATAA7318901.2989819987990776No Hit
CAGTTCACAATCCCAAGCATGAAAGTGGTTCAG6980801.2389749193480715No Hit
CATACGATCTGCATGTTATCTAGAGTTCAACCAATAT6172201.0954619810337163No Hit
TACAACCCTAAACCATATGTAGTCCAAGCA4528470.8037274743611279No Hit
TACAACCCTAACCATATGTAGTCCAAGCA4395990.7802144962905295No Hit
TACAACCCTCAACCATATGTAGTCCAAG3695520.6558928194403485No Hit
TACAACCCTGAACCATATGTAGTCCAAGCA3341940.5931382996169628No Hit
CAAGTATTGTGCCATTGGTCCGTACCTGCGGTT3122450.554182505861561No Hit
CGATTATATAACAAACTCGTGATACTTTGATCAAGAAG2567700.4557236850232127No Hit
CGTCAGAACTGTTTCGGTCTTCCATCAGGGTTT2147140.3810813385756673No Hit
CATACGATCTGCATGTTATCTAGAGTTCAACCAAT1990070.35320404792387927No Hit
CAACCCTCAACCATATGTAGTCCAAGCA1900420.3372926765166545No Hit
CTTTGTCATTGTATTAAATAATGCTTTTATATGTAACTAG1832950.3253178831106818No Hit
AGTATTTAATCACATATAAGAACTCCACCGGTAATA1706000.3027863872919737No Hit
CAGCATGGACTGCGATATGCGTTCAAAATGTCGATGTT1662490.29506409203343104No Hit
ACAACCCTCAACCATATGTAGTCCAAGCAG1659120.29446597355443105No Hit
CATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAA1607990.2853912560970813No Hit
TAAAAATTTTAGCAAGAAAGTTCCACAATTGG1598370.28368386744189444No Hit
CAAGTCAGCATTTGCCCTTTTGCTCTATGTGTGGTTT1427620.25337860622846864No Hit
CATGTTATTGTTTCCCAATCAAGCCCGACTATCT1285840.22821503413570426No Hit
AAATAATCAAGTTCGGTCAACTTTTGCGAAA1270900.22556343470654708No Hit
CTAATGCAGGTTTTTAAATAGGAGGAACATATGAAAAAATA1211290.214983659474147No Hit
GCAACCCTCAACCATATGTAGTCCAAGCA1107630.19658574803998172No Hit
CAAACTCCCTACCTGGCAATGTCCTTGAATTGGAT948410.16832686844758543No Hit
CGATCGCTTGGTTTTAGCCTAATAAAAGCACATGT943910.16752819392073087No Hit
CGCAATTTGTCCATTTAAGAAGCTAGTGTCCTTAT925240.16421458205042538No Hit
TACAACCCTCAACCATATGT824420.14632072298431942No Hit
CAACCATATGTAGTCCAAGCA807340.14328930944683588No Hit
CAGTGATCACGTCCGGAGACCTCACTAAATAATT794760.14105657043620692No Hit
CAAAGCGAGAATCATACCCCTAGACCATTGAGCC770640.13677567497226648No Hit
TAAATAATCAAGTTCGGTCAACTTTTGCGAAA768420.13638166220568487No Hit
CGATCGCTTGGTTTTAGCCTAATAAAAGCACATGTCC767030.13613496051850094No Hit
TGAGGGTTGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTG755640.13411342654941794TruSeq Adapter, Index 8 (100% over 38bp)
GATCACGTCCGGAGACCTCACTAAATAATT720170.12781809644552208No Hit
AAAAATTTTAGCAAGAAAGTTCCACAATTGG692070.12283081773338583No Hit
ACCATATGTAGTCCAAGCAG680820.12083413141624944No Hit
TTCTTAAAAATTTTAGCAAGAAAGTTCCACAATTGG662150.11752051954594396No Hit
TACAACCCTCAACCATATGTAGTCCAAGCT659570.11706261281721399No Hit
CCGACTTCCCTTACCTACATTATTCTATCGACTAGAGA652030.11572438927666213No Hit
ACAACCCTCAACCATATGTAGTCCAAG632770.1123060623017246No Hit
TACAACCCTGACCATATGTAGTCCAAGCA607530.10782638562221147No Hit
TATTCTTAAAAATTTTAGCAAGAAAGTTCCACAATTGG592930.1052351304906389No Hit
TACAACCCTAACCATATGTAGTCCAAGCAG592300.10512331605687926No Hit
TACAACCCTAAACCATATGTAGTCCAAGCAG591920.1050558724301671No Hit
CGAGAACCGATGTATTCAGCGTGGTATGGTCGTTGGC582960.10346562270558556No Hit
CTTAAAAATTTTAGCAAGAAAGTTCCACAATT580180.10297221933121764No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)