FastQCFastQC Report
Mon 3 Jul 2023
SRR5667283_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5667283_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32872272
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length20-72
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTATTTAATCACATATAAGAACTCCACCGGTAATA21050926.403853071062445No Hit
TACAACCCTCAACCATATGTAGTCCAAGCA19135815.821261761280145No Hit
CATGTTATTGTTTCCCAATCAAGCCCGACTAT15709964.77909163078232No Hit
CTAAATAATCAAGTTCGGTCAACTTTTGCGAAA13748664.182448964890532No Hit
CTTAAAAATTTTAGCAAGAAAGTTCCACAATTGG11083453.371671419608599No Hit
CATATAAGAACTCCACCGGTAATACGCTTA6657212.025174895121335No Hit
CATACGATCTGCATGTTATCTAGAGTTCAACCAATAT4555201.3857271563097313No Hit
TACAACCCTCAACCATATGTAGTCCAAGCAG4350621.3234923342079914No Hit
CAGTTCACAATCCCAAGCATGAAAGTGGTTCAG4145191.2609989355162308No Hit
CTTAAATTCAGGGGGTAGTCCCATATGAGTTGAGGTTGT3346821.0181285917809393No Hit
TACAACCCTAAACCATATGTAGTCCAAGCA3114480.9474489624568695No Hit
CATACGATCTGCATGTTATCTAGAGTTCAACCAATATAA2941190.8947328009454291No Hit
TACAACCCTAACCATATGTAGTCCAAGCA2910400.8853662442316126No Hit
AGTATTTAATCACATATAAGAACTCCACCGGTAATA2638420.8026278195799791No Hit
CTAATGCAGGTTTTTAAATAGGAGGAACATATGAAAAAATA2209000.6719949263014129No Hit
CAAGTATTGTGCCATTGGTCCGTACCTGCGGTT2186360.6651076627742676No Hit
TACAACCCTGAACCATATGTAGTCCAAGCA2093070.6367281215000898No Hit
CTTTGTCATTGTATTAAATAATGCTTTTATATGTAACTAG1727710.5255827768765116No Hit
CATACGATCTGCATGTTATCTAGAGTTCAACCAAT1592910.48457557177672417No Hit
CGATTATATAACAAACTCGTGATACTTTGATCAAGAAG1517980.4617812848469981No Hit
CATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAA1284830.390855247243026No Hit
TAAAAATTTTAGCAAGAAAGTTCCACAATTGG1230100.3742059569232087No Hit
CAGCATGGACTGCGATATGCGTTCAAAATGTCGATGTT1202440.36579157047617517No Hit
ACAACCCTCAACCATATGTAGTCCAAGCA1161950.35347419855859064No Hit
AAATAATCAAGTTCGGTCAACTTTTGCGAAA1141120.34713755106431343No Hit
CGATCGCTTGGTTTTAGCCTAATAAAAGCACATGTCCCAT1101640.3351274289772243No Hit
CATGTTATTGTTTCCCAATCAAGCCCGACTATCT983020.29904230532042325No Hit
CGTCAGAACTGTTTCGGTCTTCCATCAGGGTTT931870.28348207875622344No Hit
AATGCAGGTTTTTAAATAGGAGGAACATATGAAAAAATA896500.27272225053382376No Hit
TATTCTTAAAAATTTTAGCAAGAAAGTTCCACAATTGG880720.26792185219202375No Hit
CTTAAAAATTTTAGCAAGAAAGTTCCACAATT873130.2656129153470134No Hit
CGGTGGTGGTGAAAGAGTAGCCGCGCTCGGTCAGGAT848910.25824500357018215No Hit
ACCAAACCGAGGTCTAATATCTCCCACTTATT820290.2495385776803015No Hit
CGATCGCTTGGTTTTAGCCTAATAAAAGCACATGTCC803950.2445678229968406No Hit
GATCACGTCCGGAGACCTCACTAAATAATT799900.24333578159732921No Hit
CAAGTCAGCATTTGCCCTTTTGCTCTATGTGTGGTTT782390.23800910384289836No Hit
AAAAATTTTAGCAAGAAAGTTCCACAATTGG754870.22963730648128No Hit
CATATAAATCTATCAGCACTTTATCAAATTAATAA749460.22799154253773513No Hit
TACAACCCTCAACCATATGT710510.21614265055971793No Hit
CGATCGCTTGGTTTTAGCCTAATAAAAGCACATGT651920.19831911831345275No Hit
TACAACCCTCAACCATATGTAGTCCAAG639830.19464124658009643No Hit
CAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTT631180.19200984951694242No Hit
CAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAAT624290.18991385809900818No Hit
CGCAATTTGTCCATTTAAGAAGCTAGTGTCCTTAT608040.1849704821133142No Hit
CAACCATATGTAGTCCAAGCA562660.17116553428372705No Hit
CAAGTATTTAATCACATATAAGAACTCCACCGGTAATACGCTTA561700.17087349484087988No Hit
CAGGATGGCAGCTGGGATCGGAGGAGGAGTGGG559010.1700551759854019No Hit
CTTGTTAAAGGATTTAAAGTGTACTCATTCCAATTACAGGG547790.16664196499712583No Hit
GGTATTTAATCACATATAAGAACTCCACCGGTAATA511250.15552621370375616No Hit
TCTTAAAAATTTTAGCAAGAAAGTTCCACAATTGG510570.15531935243173942No Hit
CAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAA504270.153402843588055No Hit
CGTCACAGTGATCACGTCCGGAGACCTCACTAAATAATT502820.15296174234625462No Hit
TTTAGCAAGAAAGTTCCACAATTGG490810.14930820723313556No Hit
TACAACCCTAAACCATATGTAGTCCAAGCAG471080.1433061882671207No Hit
TACAACCCTAACCATATGTAGTCCAAGCAG467180.14211977803055414No Hit
TAATGCAGGTTTTTAAATAGGAGGAACATATGAAAAAATA465470.14159958277298265No Hit
CGGATATTGGTACGGCCTGTTGAGAAGTTTG459650.1398290936507218No Hit
CAAACTCCCTACCTGGCAATGTCCTTGAATTGGAT447700.1361938110027807No Hit
ACCATATGTAGTCCAAGCAG441740.13438073279510462No Hit
CATAGTTACTCCCGCCGTTGACCCGCGCTTA437610.13312435477535595No Hit
CAAAGCGAGAATCATACCCCTAGACCATTGAGCC430270.1308914698685871No Hit
TACAACCCTCAACCATATGTA420390.1278858972692852No Hit
TTTTAGCAAGAAAGTTCCACAATTGG420380.12788285519175555No Hit
CGTTGATACTTTCATTGTAGCGCGCGTGCAGCCCAGGA419020.12746913264772206No Hit
AAGTATTTAATCACATATAAGAACTCCACCGGTAATA418930.12744175394995516No Hit
TACAACCCTCAACCATATGTAG413840.12589333648735931No Hit
GCATGTTATCTAGAGTTCAACCAATATAA402020.12229760084730378No Hit
CACATATAAGAACTCCACCGGTAATACGCTTACATA392820.11949888952001858No Hit
CTGGTGGCGTGGACGACCGACGATGGAGGGGAAGA375790.1143182314870113No Hit
TACAACCCTGACCATATGTAGTCCAAGCA375330.11417829592064704No Hit
TACAACACTCAACCATATGTAGTCCAAGCA370590.11273635117158923No Hit
TAAATAATCAAGTTCGGTCAACTTTTGCGAAA360330.10961517962616031No Hit
AATCACATATAAGAACTCCACCGGTAATA352360.10719064383502303No Hit
TGTTATTGTTTCCCAATCAAGCCCGACTAT344700.10486041244730512No Hit
TAAGTATTTAATCACATATAAGAACTCCACCGGTAATA343210.1044071428953861No Hit
CTTCATCGTCACACATTTTGTTTTAGTTTTGTTTTTTTTA341580.10391128425805188No Hit
CATAGAGGTTCTAGTGATGACGGGGGTGCTCGGGTG340410.10355536118708192No Hit
CAGCTGCTCAACCACTTACAACACCTTGCCTGTT335030.101918723476126No Hit
TACAACCCTCAACCATATGTAGTCCA334970.10190047101094808No Hit
TACAACCTCAACCATATGTAGTCCAAGCA333060.10131943420278343No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)