FastQCFastQC Report
Tue 4 Jul 2023
SRR5667284_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5667284_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69053087
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length20-76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTATTTAATCACATATAAGAACTCCACCGGTAATA41133455.956786551772842No Hit
CTTAAAAATTTTAGCAAGAAAGTTCCACAATTGG37843125.480293734007866No Hit
TACAACCCTCAACCATATGTAGTCCAAGCA37606575.446037481278716No Hit
CATGTTATTGTTTCCCAATCAAGCCCGACTAT24078303.486925935693505No Hit
CTAAATAATCAAGTTCGGTCAACTTTTGCGAAA20725263.001351699164441No Hit
CATATAAGAACTCCACCGGTAATACGCTTA12650731.8320296093352062No Hit
CATACGATCTGCATGTTATCTAGAGTTCAACCAATAT11077841.6042497853861335No Hit
TACAACCCTAAACCATATGTAGTCCAAGCA10632921.5398181981350088No Hit
TACAACCCTAACCATATGTAGTCCAAGCA9581691.3875831503376526No Hit
TACAACCCTCAACCATATGTAGTCCAAGCAG7894061.1431871250013774No Hit
CTAATGCAGGTTTTTAAATAGGAGGAACATATGAAAAAATA7602611.100980467390256No Hit
CATACGATCTGCATGTTATCTAGAGTTCAACCAATATAA7170141.0383518408090864No Hit
CAGTTCACAATCCCAAGCATGAAAGTGGTTCAG6905601.0000421849351935No Hit
TACAACCCTGAACCATATGTAGTCCAAGCA6702650.9706517537731514No Hit
CTTAAATTCAGGGGGTAGTCCCATATGAGTTGAGGTTGT5111510.740229035669325No Hit
AGTATTTAATCACATATAAGAACTCCACCGGTAATA4838260.7006580314070535No Hit
CGATCGCTTGGTTTTAGCCTAATAAAAGCACATGTCCCAT4706320.6815509927890696No Hit
CTTTGTCATTGTATTAAATAATGCTTTTATATGTAACTAG4353760.6304946222027699No Hit
TAAAAATTTTAGCAAGAAAGTTCCACAATTGG4037530.5846994211859058No Hit
CGATTATATAACAAACTCGTGATACTTTGATCAAGAAG3752210.5433804863785452No Hit
CATACGATCTGCATGTTATCTAGAGTTCAACCAAT3599260.5212308611199381No Hit
CAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAAT3515860.5091531968730088No Hit
CAAGTATTGTGCCATTGGTCCGTACCTGCGGTT3381540.48970149589402134No Hit
CTTGTTAAAGGATTTAAAGTGTACTCATTCCAATTACAGGG3357190.4861752234190486No Hit
ACAACCCTCAACCATATGTAGTCCAAGCA2815670.4077543991624878No Hit
AATGCAGGTTTTTAAATAGGAGGAACATATGAAAAAATA2654070.38435211448258644No Hit
TATTCTTAAAAATTTTAGCAAGAAAGTTCCACAATTGG2590160.37509691637681597No Hit
CATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAA2323240.3364425981419194No Hit
CAGCATGGACTGCGATATGCGTTCAAAATGTCGATGTT2189920.31713571328100076No Hit
AAAAATTTTAGCAAGAAAGTTCCACAATTGG2046590.2963792190782144No Hit
CTTAAAAATTTTAGCAAGAAAGTTCCACAATT1989520.28811456322003387No Hit
CGCAATTTGTCCATTTAAGAAGCTAGTGTCCTTAT1905330.2759224942398303No Hit
CATGTTATTGTTTCCCAATCAAGCCCGACTATCT1627380.235670854222636No Hit
CGATCGCTTGGTTTTAGCCTAATAAAAGCACATGTCC1625660.23542177049955784No Hit
TCTTAAAAATTTTAGCAAGAAAGTTCCACAATTGG1605900.23256020400652036No Hit
AAATAATCAAGTTCGGTCAACTTTTGCGAAA1574030.227944914323671No Hit
TTTAGCAAGAAAGTTCCACAATTGG1536360.22248969115602318No Hit
CGTCAGAACTGTTTCGGTCTTCCATCAGGGTTT1518160.2198540378071729No Hit
TACAACCCTAAACCATATGTAGTCCAAGCAG1499580.21716335433345654No Hit
CAAGTCAGCATTTGCCCTTTTGCTCTATGTGTGGTTT1497390.21684620703488608No Hit
CATATAAATCTATCAGCACTTTATCAAATTAATAA1466930.21243510807851357No Hit
TACAACCCTAACCATATGTAGTCCAAGCAG1414040.20477578359386017No Hit
TAATGCAGGTTTTTAAATAGGAGGAACATATGAAAAAATA1365240.1977087570321078No Hit
TTTTAGCAAGAAAGTTCCACAATTGG1349470.19542500685016442No Hit
CGGTGGTGGTGAAAGAGTAGCCGCGCTCGGTCAGGAT1343290.19453004324049988No Hit
TTCTTAAAAATTTTAGCAAGAAAGTTCCACAATTGG1339000.19390878209398515No Hit
GATCACGTCCGGAGACCTCACTAAATAATT1297710.18792932457892866No Hit
CGTCACAGTGATCACGTCCGGAGACCTCACTAAATAATT1273770.18446242671236407No Hit
TACAACCCTGACCATATGTAGTCCAAGCA1213750.17577056330588087No Hit
TACAACCCTCAACCATATGTAGTCCAAG1205480.17457293400945276No Hit
CGTTGATACTTTCATTGTAGCGCGCGTGCAGCCCAGGA1173150.16989102891229177No Hit
CAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTT1105170.16004642920598178No Hit
CAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAA1070270.15499234668538425No Hit
CGATCGCTTGGTTTTAGCCTAATAAAAGCACATGT1031220.14933727727480164No Hit
TACAACCCTACCATATGTAGTCCAAGCA983730.14245995982771922No Hit
TACAACCCTCAACCATATGT959050.1388858980337838No Hit
TACAACCCTGAACCATATGTAGTCCAAGCAG931810.13494110697759246No Hit
TACAACACTCAACCATATGTAGTCCAAGCA931310.13486869891855813No Hit
CTGCATGTTATCTAGAGTTCAACCAATATAA909780.1317508078965391No Hit
CGCAATTTGTCCATTTAAGAAGCTAGTGTCCTTATAAT890000.12888634508114025No Hit
CAAACTCCCTACCTGGCAATGTCCTTGAATTGGAT875720.12681837091511927No Hit
ATTCTTAAAAATTTTAGCAAGAAAGTTCCACAATTGG869430.12590747753246717No Hit
GCATGTTATCTAGAGTTCAACCAATATAA856470.12403066064229686No Hit
CAACCATATGTAGTCCAAGCA820320.11879555797411345No Hit
CTTCATCGTCACACATTTTGTTTTAGTTTTGTTTTTTTTA811420.11750669452330205No Hit
AAGTATTTAATCACATATAAGAACTCCACCGGTAATA804390.11648863721327911No Hit
TACAACCTCAACCATATGTAGTCCAAGCA798830.11568345959681715No Hit
ACCAAACCGAGGTCTAATATCTCCCACTTATT780630.11304780624796687No Hit
CAAAGCGAGAATCATACCCCTAGACCATTGAGCC741530.10738549603148083No Hit
TAAATAATCAAGTTCGGTCAACTTTTGCGAAA735620.10652963277369483No Hit
GGTATTTAATCACATATAAGAACTCCACCGGTAATA701470.10158416234164883No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)