FastQCFastQC Report
Mon 14 Aug 2023
SRR5720007_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5720007_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16210757
Total Bases810.5 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGA11352467.003041252175947No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGAT7084574.370289431887727No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAGAT5121473.1593034180945403No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATC4755662.933644616349502No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAGATC2902541.7905024422980371No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCG2602341.605316765898101No Hit
CTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCAC2545131.570025384995901No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCTGTAGGCACCATCAATAGATC2511331.5491750323566014No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAATAGATCG2489391.5356408093712095No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2443871.507560689485383Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAATAGATCGG2407181.4849275700079891No Hit
GGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGA2360041.4558481136938886No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCG1977671.2199738729042695No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAG1893611.1681194160149337No Hit
GGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAA1879411.159359800409074No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAGATCG1869881.153480987963733No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCTGTAGGCACCATCAATAGATCG1211210.7471643674629136No Hit
CGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGG1189550.7338028692922853No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAATAGATCGGA963910.5946113435664973No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAGATCGG945810.5834459180407182No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATAGATC874850.5396725149849572No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAGA852980.5261814732032564No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCTGTAGGCACCATCAATAGATCGG840630.5185630751235121No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA825540.50925444135644No Hit
ATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCAC815710.5031905666095667No Hit
GGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGA781160.4818775582164362No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA769460.4746601284566785No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGG729190.44981859884766645No Hit
GGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAG647390.3993582779632068No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGG635480.3920113045923765No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATAGATCG596920.36822463010209827No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGA593710.3662444634757032No Hit
GATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCA582830.359532870673467No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG581640.3587987902107224No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAATA573190.35358620205089747No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT557270.34376556258291946No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGG548080.33809648741264825No Hit
GCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAG543240.33511081561459466No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAATAGATC533720.329238171912638No Hit
CTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA527410.32534569483707637No Hit
GCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGAAGAGC509530.3143159816657544No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG472340.29137442501914007No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCTGTAGGCACCATCAATAGATCGGA444380.2741266185163346No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGCCTGTAGGCACCATCAATAGAT439340.2710175718505928No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCTGTAGGCACCATCAATAGAT418960.2584456728331687No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATC388560.2396926929445676No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA374770.23118599581746863No Hit
TCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAATAGATCGGAA361350.22290754219559272No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGG342920.21153854813812828No Hit
CGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAGATCGGA340350.20995318108833536No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAAT316550.19527157183344368No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCTGTAGGCACCATCAATAG296580.18295259129478036No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGCCTGTAGGCACCATCAATAGATC292780.18060846880870524No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT288850.1781841526586328No Hit
GTGAAATACCACTACTCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC285160.17590788634978613No Hit
GGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGC265760.1639405241840341No Hit
GGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGA255220.1574386686568678No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA252960.15604453265199153No Hit
GCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCA252350.1556682393055426No Hit
CCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATAGATCGGAAGAG243870.1504371449155644No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC223150.13765550862307047No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAG219830.13560748581944693No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC219300.13528054242007329No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATAGATCGGA218190.13459581190440398No Hit
GGGCCGCCGGTGAAATACCACTACTCTCTGTAGGCACCATCAATAGATCG213120.13146825900850898No Hit
GTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGAA207840.1282111625015414No Hit
GGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAGATCGGAA206840.1275942881631006No Hit
CGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC200880.12391771710599327No Hit
CCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAG196370.12113561383962512No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGGA196160.12100607022855256No Hit
GGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATCGG191620.1182054607320312No Hit
TCGCCCGTCACGCACCGCACGTTCGTGGGGACTGTAGGCACCATCAATAG184970.11410324638139971No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGG182330.11247469812791593No Hit
CCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGGA179670.11083381238766332No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA178550.1101429131286096No Hit
GCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC177090.10924227659448599No Hit
CCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAATAGATCGGAAGA175910.10851436487512582No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCA169300.10443682549803196No Hit
GGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG163290.10072941072400259No Hit
TCGCCCGTCACGCACCGCACGTTCCTGTAGGCACCATCAATAGATCGGAA162270.10010019889879293No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)