FastQCFastQC Report
Mon 3 Jul 2023
SRR5720008_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5720008_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29822088
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length35-50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA316946210.627901037646994Illumina PCR Primer Index 6 (96% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCTGTAGGCACCATCAATATCTCG5787381.9406354109075123No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCATCAATATCTC4374091.4667282854238777No Hit
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA3839941.2876160783912916Illumina PCR Primer Index 6 (100% over 28bp)
CCGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGCTGTAGGCACCATC2536600.850577598724811No Hit
AGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCTC2419240.8112242174323945No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCTGTAGGCACCATCAATATCTCG2402190.8055069785858052No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGTAGGCACCATCAATATCT2210150.7411117558234018No Hit
GCATTGGTGGTTCAGTGGTAGAATTCCTGTAGGCACCATCAATATCTCGT1784030.5982243765091163No Hit
CGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA1737850.5827392099439851Illumina PCR Primer Index 6 (96% over 29bp)
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTGTAGGCACCAT1667330.5590923076881806No Hit
CGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGCTGTAGGCACCATCA1663710.5578784423143007No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCCTGTAGGCACCATCAATATCTCG1599480.5363407149760943No Hit
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGCTGTAGGCACCATCAAT1470840.4932049023529137No Hit
GTATAGTGGTGAGTATCCCTGTAGGCACCATCAATATCTCGTATGCCGTC1469270.4926784469283304No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATATC1396360.46823012526822405No Hit
TTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA1388750.4656783254076643Illumina PCR Primer Index 6 (96% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCTGTAGGCACCATCAATATCTCGT1386300.4648567866877732No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAT1260250.42258945785419183No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT1243410.4169426366121648No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1232180.41317697137772513Illumina PCR Primer Index 6 (100% over 28bp)
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACTGTAGGCACCATCA1019930.3420048924810362No Hit
GCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA972920.3262414087169215Illumina PCR Primer Index 6 (100% over 28bp)
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT968150.3246419231275825No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT902750.30271186913538717No Hit
CTCTCGGGGCCACGCGCGCGTCCCCCGCTGTAGGCACCATCAATATCTCG878000.2944126514548545No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGTAGGCACCATCAATATCT870050.29174684213928953No Hit
GTATAGTGGTGAGTATCCTGTAGGCACCATCAATATCTCGTATGCCGTCT867360.2908448261570417Illumina PCR Primer Index 6 (95% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT824070.27632873995945556No Hit
CGCGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGCTGTAGGCACCATC818130.2743369277161277No Hit
CGGGGCCACGCGCGCGTCCCCCGCTGTAGGCACCATCAATATCTCGTATG784010.2628957435844197No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT776920.2605183111256328No Hit
CCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGCTGTAGGCACCATCAAT761500.2553476470192161No Hit
TCCCTGGTGGTCTAGTGGTTAGGATCTGTAGGCACCATCAATATCTCGT741490.2486378552702279No Hit
TCTCGGGGCCACGCGCGCGTCCCCCGCTGTAGGCACCATCAATATCTCGT725740.24335653492807077No Hit
CTCTCGGGGCCACGCGCGCGTCCCCCCTGTAGGCACCATCAATATCTCGT711480.23857484425637804No Hit
GCATGGGTGGTTCAGTGGTAGAATTCCTGTAGGCACCATCAATATCTCGT681070.2283777044719337No Hit
CGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTGTAGGCACCATC676410.22681510429450816No Hit
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCTGTAGGCACCATC645500.21645030354682074No Hit
GTATAGTGGTGAGTATCCCCTGTAGGCACCATCAATATCTCGTATGCCGT629250.2110013222414205No Hit
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCTGTAGGCACCATC620300.20800019099936934No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATATCT593500.19901356336954004No Hit
AGGGGCCCGGGGCGGGGTCCGCCGGCCTGTAGGCACCATCAATATCTCGT568270.19055339116429407No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATATC562140.18849786775493385No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTGTAGGCACCATCAATATCTCGT557400.18690844182338945No Hit
TCTCGGGGCCACGCGCGCGTCCCCCCTGTAGGCACCATCAATATCTCGT548330.1838670719501599No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAATAT541560.18159694250784855No Hit
TCTCTCGGGGCCACGCGCGCGTCCCCCGCTGTAGGCACCATCAATATCTC527600.17691584841410166No Hit
TCCCCGCGAGGGGCCCGGGGCGGGGTCCGCCGGCCTGTAGGCACCATCA526240.17645981059408047No Hit
TTCCCGGCCAATGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTG521830.17498104089827646Illumina PCR Primer Index 6 (96% over 25bp)
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAT511500.17151716539767436No Hit
TCGGGGCCACGCGCGCGTCCCCCGCTGTAGGCACCATCAATATCTCGTAT493870.16560543983372325No Hit
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA488900.163938889859087Illumina PCR Primer Index 6 (96% over 29bp)
CCGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGCCTGTAGGCACCATC479810.16089081354732773No Hit
CGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGCTGTAGGCACCATCAAT475380.159405337412994No Hit
CGGGCGCTGACCCCCTTCGCGGGGGGGATGCGCTGTAGGCACCATCAAT459060.1539328835727398No Hit
AGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATATCTCGT452190.1516292219377798No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT451680.15145820775527186No Hit
CGGCGGCGGGGGCACGGTCTGTAGGCACCATCAATATCTCGTATGCCGTC433510.14536540835101822No Hit
AGGGGCCCGGGGCGGGGTCCGCCGGCTGTAGGCACCATCAATATCTCGT411490.13798161952979282No Hit
CGCCCCCGGGGCCGCGGTTCTGTAGGCACCATCAATATCTCGTATGCCGT410660.1377033023308093No Hit
CCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATATCTCGTATGC406620.1363486017478052No Hit
TTCCCGGTCAGGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC402350.13491677712170924Illumina PCR Primer Index 6 (100% over 25bp)
CGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACTGTAGGCACCATCAAT402240.13487989170979578No Hit
GCCCCCGGGGCCGCGGTTCTGTAGGCACCATCAATATCTCGTATGCCGTC402230.1348765384905309No Hit
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCCTGTAGGCACC393700.1320162424576039No Hit
CGGGGCCACGCGCGCGTCCCCCCTGTAGGCACCATCAATATCTCGTATGC387610.12997413192530313No Hit
GTCTAGTGGTTAGGATTCGCTGTAGGCACCATCAATATCTCGTATGCCGT385580.12929342841453623No Hit
TTCCCGGCCCATGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTG385230.12917606574026608Illumina PCR Primer Index 6 (96% over 25bp)
CTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATATCTC385030.12910900135496883No Hit
TCCCTGGTGGTCTAGTGGTTAGGACTGTAGGCACCATCAATATCTCGTAT380460.12757658015092704No Hit
TTCTCTCGGGGCCACGCGCGCGTCCCCCGCTGTAGGCACCATCAATATCT380140.12746927713445147No Hit
CGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGCCTGTAGGCACCATC376310.12618499415600948No Hit
CTCGGGGCCACGCGCGCGTCCCCCGCTGTAGGCACCATCAATATCTCGT353480.11852959457433028No Hit
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCCTGTAGGCACCATCAAT350660.11758398674163928No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAAT344140.11539768778094947No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGCTGTAGGCACCATCAATATCTC341650.11456273618399891No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAAT328080.11001241764158162No Hit
GTATAGTGGTTAGTATCCCTGTAGGCACCATCAATATCTCGTATGCCGTC326100.10934848022713901No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGTAGGCACCATCAAT317300.10639764727406076No Hit
CGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCCTGTAGGCACCATCAAT316470.10611933007507723No Hit
GTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATATCTCGT313950.10527431882033211No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATATCT312620.10482834065810549No Hit
CCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA310870.1042415272867547Illumina PCR Primer Index 6 (100% over 28bp)
GGGGCCACGCGCGCGTCCCCCGCTGTAGGCACCATCAATATCTCGTATGC308930.10359100274937154No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAATATCTCGT307890.10324226794582593No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)