FastQCFastQC Report
Mon 3 Jul 2023
SRR5720009_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5720009_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35932206
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length35-50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA1114408031.014182652743333Illumina PCR Primer Index 6 (96% over 29bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT4583081.2754797186679827No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATATC4162481.1584259535860393No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2813190.7829160280334584Illumina PCR Primer Index 6 (100% over 28bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA2593890.7218844286933009Illumina PCR Primer Index 6 (96% over 29bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT2191570.6099180217323702No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT2035500.5664834494158248No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT1839250.5118667081002486No Hit
CTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA1744800.4855810968021279Illumina PCR Primer Index 6 (96% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCATCAATATCTC1734620.48274798380038236No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1720020.47868477654837No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAAT1617840.4502478918216154No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAT1499780.417391573453631No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATATCT1096490.3051552136821213No Hit
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGCTGTAGGCACCATCAAT1063860.29607422377574033No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATATCT939860.2615647923202934No Hit
CGCGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGCTGTAGGCACCATC936550.26064361314192624No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCTGTAGGCACCATCAATATCTCG925290.25750993412427836No Hit
CCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA776650.2161431446763942Illumina PCR Primer Index 6 (96% over 29bp)
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA734960.20454073985883303Illumina PCR Primer Index 6 (96% over 29bp)
CCGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGCTGTAGGCACCATC716340.19935875910318449No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT707770.19697371210662656No Hit
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTGTAGGCACCAT693010.19286597655596208No Hit
GCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA688870.19171380682833666Illumina PCR Primer Index 6 (100% over 28bp)
CGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATATC667660.1858110242382558No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCTGTAGGCACCATCAATATCTCGT667030.18563569406231278No Hit
CGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA661260.1840298922921682Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA649830.1808489019572024Illumina PCR Primer Index 6 (96% over 29bp)
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT626960.17448413826860504No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT626910.17447022317527625No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGTAGGCACCATCAATATCT616600.17160093093087578No Hit
CGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGCTGTAGGCACCATCA607400.16904055375837487No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAAT597170.1661935256633005No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATATCTC593030.1650413559356751No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT564780.15717932820489786No Hit
TTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA554330.15427107369917673Illumina PCR Primer Index 6 (96% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCCTGTAGGCACCATCAATATCTCGT552730.15382579071265481No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCCTGTAGGCACCATCAATATCTCG544740.1516021587987111No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT536970.14943975329541415No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCA536290.14925050802614234No Hit
AGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCTC533370.14843786657573987No Hit
CCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATATCTCGTATGC528890.14719107421347857No Hit
TCCCTGGTGGTCTAGTGGTTAGGATCTGTAGGCACCATCAATATCTCGT520210.14477541401159727No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAATATCTCGT498360.13869451822690765No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAATATCTCGT497140.1383549899496847No Hit
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA473480.13177036778649215Illumina PCR Primer Index 6 (100% over 28bp)
CCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA460760.12823036804364307Illumina PCR Primer Index 6 (100% over 28bp)
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT451410.12562824559115573No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGCCTGTAGGCACCATCAATATCT450120.12526923618327246No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT431020.11995367053166733No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAT428970.11938315170518614No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATATC425920.11853433101212878No Hit
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA417330.11614371797823936Illumina PCR Primer Index 6 (100% over 28bp)
TGCTGGGCCCATAACCCAGAGGTCGATGGCTGTAGGCACCATCAATATCT411410.11449617092810835No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAATATCTCG405250.11278183142999904No Hit
AGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATATCTCGT396500.11034669009745743No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATC384960.10713508655716825No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAATAT382300.10639480359207558No Hit
TCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA379010.10547919045103993Illumina PCR Primer Index 6 (100% over 28bp)
CCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATATCTC375730.10456636032867006No Hit
CGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA373470.10393739811020788Illumina PCR Primer Index 6 (96% over 29bp)
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAT362930.10100409643649488No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)