FastQCFastQC Report
Thu 28 Sept 2023
SRR5742252.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5742252.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22928616
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCTGAGGCGTGATGACGAGGCTGGAATTCTCGGGTGCCAAGGAACTCC21532569.391129407897973RNA PCR Primer, Index 1 (100% over 27bp)
AAGGCTGAGGCGTGATGACGAGGCTGGAATTCTCGGGTGCCAAGGAACTC15708716.851137460717211RNA PCR Primer, Index 1 (100% over 26bp)
AAGGCTGAGGCGTGATGACGAGGCACTGGAATTCTCGGGTGCCAAGGAAC11251144.907029713437566RNA PCR Primer, Index 1 (100% over 24bp)
AGGCTGAGGCGTGATGACGAGGCACTGGAATTCTCGGGTGCCAAGGAACT5874452.562060440106808RNA PCR Primer, Index 1 (100% over 25bp)
AAACGATGTCGACTTGGAGGTTGTGCCTGGAATTCTCGGGTGCCAAGGAA5800672.5298823095122707RNA PCR Primer, Index 1 (100% over 23bp)
AATGAACCGTGAGGCTTAACCTGGAATTCTCGGGTGCCAAGGAACTCCAG4180891.8234375768690094RNA PCR Primer, Index 1 (100% over 29bp)
ATGCACCGAAGCTGCGGCAGCGACGCTGGAATTCTCGGGTGCCAAGGAAC3464481.5109852247514635RNA PCR Primer, Index 1 (100% over 24bp)
GATGTCGACTTGGAGGTTGTGCCTGGAATTCTCGGGTGCCAAGGAACTCC3386671.4770494651748713RNA PCR Primer, Index 1 (100% over 27bp)
AAGTCGAACGGTAACAGGAAGAAGCTGGAATTCTCGGGTGCCAAGGAACT2605451.136331124390587RNA PCR Primer, Index 1 (100% over 25bp)
AACCGGTACTAATGAACCGTGAGGCTTAACCTGGAATTCTCGGGTGCCAA2604451.135894988166752No Hit
ACGTACAAGCAGTGGGAGCACGCTGGAATTCTCGGGTGCCAAGGAACTCC2440821.0645300178606507RNA PCR Primer, Index 1 (100% over 27bp)
AAGTCGAACGGTAACAGGAAACAGCTGGAATTCTCGGGTGCCAAGGAACT1906530.8315067948279128RNA PCR Primer, Index 1 (100% over 25bp)
ACCGAAGCTGCGGCAGCGACGCTGGAATTCTCGGGTGCCAAGGAACTCCA1716350.7485624077789955RNA PCR Primer, Index 1 (100% over 28bp)
ATGCGAGAGTAGGGAACTGCCAGGCATGGAATTCTCGGGTGCCAAGGAAC1700120.7414839168661553RNA PCR Primer, Index 1 (100% over 24bp)
ACAAGCAGTGGGAGCACGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1673660.7299437523834845RNA PCR Primer, Index 1 (100% over 31bp)
AAGCAGTGGGAGCACGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1525150.6651731617817666RNA PCR Primer, Index 1 (100% over 33bp)
ATCTCCGGGGGTAGAGCACTGTTTCGGCTGGAATTCTCGGGTGCCAAGGA1468340.6403962629057071RNA PCR Primer, Index 1 (100% over 22bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGC1466200.6394629313867003RNA PCR Primer, Index 4 (100% over 50bp)
AAACGATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAGGA1410960.6153707663820616RNA PCR Primer, Index 1 (100% over 22bp)
AATGAGGCGAACCGGGGGAACTGAAACTGGAATTCTCGGGTGCCAAGGAA1132810.49405947572238984RNA PCR Primer, Index 1 (100% over 23bp)
GATGACGAGGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAC1110690.4844121424511624RNA PCR Primer, Index 4 (100% over 37bp)
ACTGGAACTGAGACACGGTCCAGACTCCTGGAATTCTCGGGTGCCAAGGA986310.43016551893057997RNA PCR Primer, Index 1 (100% over 22bp)
GATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAGGAACTC950010.4143337740053739RNA PCR Primer, Index 1 (100% over 26bp)
ACTGTTTCGGCAAGGGGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT861330.37565721367569677RNA PCR Primer, Index 1 (100% over 30bp)
AGGCTGAGGCGTGATGACGAGGTGGAATTCTCGGGTGCCAAGGAACTCCA780190.34026912047373464RNA PCR Primer, Index 1 (100% over 28bp)
CATGCGAGAGTAGGGAACTGCCAGGCATGGAATTCTCGGGTGCCAAGGAA773110.33718127600898373RNA PCR Primer, Index 1 (100% over 23bp)
AATGAACCGTGAGGCTTAACTGGAATTCTCGGGTGCCAAGGAACTCCAGT740230.32284111696929285RNA PCR Primer, Index 1 (100% over 30bp)
AAGGCTGAGGCGTGATGACGAGGCATGGAATTCTCGGGTGCCAAGGAACT729650.31822679572111984RNA PCR Primer, Index 1 (100% over 25bp)
CGGTACTAATGAACCGTGAGGCTTAACCTGGAATTCTCGGGTGCCAAGGA715010.3118417614041772RNA PCR Primer, Index 1 (100% over 22bp)
AGGGAACTGCCAGGCATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA696990.3039825866506727RNA PCR Primer, Index 1 (100% over 32bp)
ACGGGAGACACACGGCGGGTGCTGGAATTCTCGGGTGCCAAGGAACTCCA690650.30121748299155954RNA PCR Primer, Index 1 (100% over 28bp)
GAGGCGTGATGACGAGGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGT690350.3010866421244091RNA PCR Primer, Index 1 (100% over 30bp)
ATGCAAGTCGAACGGTAACAGGAAACAGCTGGAATTCTCGGGTGCCAAGG684770.29865300199541045Illumina Small RNA Adapter 2 (100% over 21bp)
AGGAAAAGCCTCTAAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC681120.29706110477841313RNA PCR Primer, Index 1 (100% over 33bp)
AGGGAACTGCCAGGCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT662060.2887483483521203RNA PCR Primer, Index 3 (100% over 34bp)
AGGGAACTGCCAGGCATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC657070.28657202859518427RNA PCR Primer, Index 1 (100% over 33bp)
AGGCTGAGGCGTGATGACGAGGCATGGAATTCTCGGGTGCCAAGGAACTC620210.2704960473846306RNA PCR Primer, Index 1 (100% over 26bp)
ATGCAAGTCGAACGGTAACAGGAAGAAGCTGGAATTCTCGGGTGCCAAGG616130.2687166115913843Illumina Small RNA Adapter 2 (100% over 21bp)
AAACGATGTCGACTTGGAGGTTGTGCTGGAATTCTCGGGTGCCAAGGAAC596620.2602075938643658RNA PCR Primer, Index 1 (100% over 24bp)
GGGGGTAGAGCACTGTTTCGGCTGGAATTCTCGGGTGCCAAGGAACTCCA591180.25783501280670407RNA PCR Primer, Index 1 (100% over 28bp)
GTCGACTTGGAGGTTGTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT571250.2491428178656749RNA PCR Primer, Index 1 (100% over 30bp)
AAGGCTGAGGCGTGATGACGAGGTGGAATTCTCGGGTGCCAAGGAACTCC538270.23475904520360058RNA PCR Primer, Index 1 (100% over 27bp)
ACGAGAGGACCGGAGTGGACGCTGGAATTCTCGGGTGCCAAGGAACTCCA517680.22577900035484041RNA PCR Primer, Index 1 (100% over 28bp)
GAAAGCTATTTAGGTAGCGCCTCGTGATGGAATTCTCGGGTGCCAAGGAA508800.2219061106871867RNA PCR Primer, Index 1 (100% over 23bp)
AGAGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCT503560.21962075687429194RNA PCR Primer, Index 4 (100% over 43bp)
ATGCGAGAGTAGGGAACTGCCAGACATGGAATTCTCGGGTGCCAAGGAAC479460.20910987387987132RNA PCR Primer, Index 1 (100% over 24bp)
GTACAAGCAGTGGGAGCACGCTGGAATTCTCGGGTGCCAAGGAACTCCAG454380.19817157738609256RNA PCR Primer, Index 1 (100% over 29bp)
GAGAGTAGGGAACTGCCAGGCATGGAATTCTCGGGTGCCAAGGAACTCCA444040.1936619288316399RNA PCR Primer, Index 1 (100% over 28bp)
AGGTAGCGCCTCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT442420.1929553881490274RNA PCR Primer, Index 3 (100% over 34bp)
CTGCTTCCAGGAAAAGCCTCTAAGCTGGAATTCTCGGGTGCCAAGGAACT436790.19049994120883704RNA PCR Primer, Index 1 (100% over 25bp)
GAACGGTAACAGGAAGAAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT424460.18512238156895294RNA PCR Primer, Index 1 (100% over 30bp)
AACGTCGCAAGACCAAAGAGGGGGATGGAATTCTCGGGTGCCAAGGAACT418040.18232238701193304RNA PCR Primer, Index 1 (100% over 25bp)
AGCGAAATTCCTTGTCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT416310.18156787134469868RNA PCR Primer, Index 1 (100% over 30bp)
ATGCGAGAGTAGGGAACTGCCAGGCATTGGAATTCTCGGGTGCCAAGGAA393590.17165885633917022RNA PCR Primer, Index 1 (100% over 23bp)
GAGGCGAACCGGGGGAACTGAAACTGGAATTCTCGGGTGCCAAGGAACTC389770.16999281596412097RNA PCR Primer, Index 1 (100% over 26bp)
CGGGGGTAGAGCACTGTTTCGGCTGGAATTCTCGGGTGCCAAGGAACTCC388890.1696090160871463RNA PCR Primer, Index 1 (100% over 27bp)
AAATGCGGAAGAGATAAGTGCTGAAAGCTGGAATTCTCGGGTGCCAAGGA381740.1664906420867269RNA PCR Primer, Index 1 (100% over 22bp)
TGAGGCGAACCGGGGGAACTGATGGAATTCTCGGGTGCCAAGGAACTCCA380660.16601961496498524RNA PCR Primer, Index 1 (100% over 28bp)
CGACTTGGAGGTTGTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA369270.16105202337550598RNA PCR Primer, Index 1 (100% over 32bp)
AAGTCGAACGGTAACAGGAAGAAGTGGAATTCTCGGGTGCCAAGGAACTC367300.16019283501455125RNA PCR Primer, Index 1 (100% over 26bp)
GCGGGAATAGCTCAGTTGGTAGAGCACGACCTGGAATTCTCGGGTGCCAA352090.1535592030500227No Hit
GAACCGTGAGGCTTAACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA345300.1505978380901839RNA PCR Primer, Index 1 (100% over 32bp)
GATGTCGACTTGGAGGTTGTGCTGGAATTCTCGGGTGCCAAGGAACTCCA327290.1427430246989177RNA PCR Primer, Index 1 (100% over 28bp)
AATGAACCGTGAGGCTTAACCTTGGAATTCTCGGGTGCCAAGGAACTCCA326720.14249442705133183RNA PCR Primer, Index 1 (100% over 28bp)
CCATGCGAGAGTAGGGAACTGCCAGGCATGGAATTCTCGGGTGCCAAGGA311980.1360657791120057RNA PCR Primer, Index 1 (100% over 22bp)
ACGGGAGACACACGGCGGGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG304900.13297793464725477RNA PCR Primer, Index 1 (100% over 29bp)
GAACGGTAACAGGAAACAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT297350.12968510615730142RNA PCR Primer, Index 1 (100% over 30bp)
ACTGGAACTGAGACACGGTCCAGACTCCTTGGAATTCTCGGGTGCCAAGG297090.12957171073910437Illumina Small RNA Adapter 2 (100% over 21bp)
ATCGGATGTGCCCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT297090.12957171073910437RNA PCR Primer, Index 3 (100% over 34bp)
ACTGTTTCGGCAAGGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA295230.12876049736277148RNA PCR Primer, Index 1 (100% over 32bp)
AAGTCGAACGGTAACAGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCAG292750.127678879527661RNA PCR Primer, Index 1 (100% over 29bp)
GATGTCGACTTGGAGGTTGTGCCGATGACGAGGCACTGGAATTCTCGGGT291360.1270726501765305No Hit
ATTCCTGTACTTGGTGTTACTGGAATTCTCGGGTGCCAAGGAACTCCAGT290050.1265013117233068RNA PCR Primer, Index 1 (100% over 30bp)
CCTGCTTCCAGGAAAAGCCTCTAAGCTGGAATTCTCGGGTGCCAAGGAAC287890.12555925747982347RNA PCR Primer, Index 1 (100% over 24bp)
AGTGGGAGCACGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAC286250.12484399407273426RNA PCR Primer, Index 4 (100% over 37bp)
GACTTGGAGGTTGTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC284210.12395427617611111RNA PCR Primer, Index 1 (100% over 33bp)
ATTCATCTCCGGGGGTAGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAG281920.12295552422352923RNA PCR Primer, Index 1 (100% over 29bp)
CGAACGGTAACAGGAAGAAGCTGGAATTCTCGGGTGCCAAGGAACTCCAG280830.1224801357395492RNA PCR Primer, Index 1 (100% over 29bp)
ACGGTCCAGACTCCTACGGGAGGCTGGAATTCTCGGGTGCCAAGGAACTC279120.12173434279679157RNA PCR Primer, Index 1 (100% over 26bp)
ACGAGAGGACCGGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG266460.116212858203042RNA PCR Primer, Index 4 (100% over 35bp)
AAGTCGAACGGTAACAGGAAGCAGCTGGAATTCTCGGGTGCCAAGGAACT264260.11525335851060527RNA PCR Primer, Index 1 (100% over 25bp)
ATCTCCGGGGGTAGAGCACTGTTTCGGTGGAATTCTCGGGTGCCAAGGAA261030.1138446385076186RNA PCR Primer, Index 1 (100% over 23bp)
AGTCGAAGATACCAGCTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT255790.11155928469472384RNA PCR Primer, Index 1 (100% over 30bp)
ATTTAGGTAGCGCCTCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGT255360.11137174611847483RNA PCR Primer, Index 1 (100% over 30bp)
GGTACTAATGAACCGTGAGGCTTAACCTGGAATTCTCGGGTGCCAAGGAA254910.11117548481774914RNA PCR Primer, Index 1 (100% over 23bp)
AAACGATGTCGACTTGGAGGTGCCTGGAATTCTCGGGTGCCAAGGAACTC250470.10923903998392226RNA PCR Primer, Index 1 (100% over 26bp)
GAGAGTAGGGAACTGCCAGGCATTGGAATTCTCGGGTGCCAAGGAACTCC246520.10751630189977451RNA PCR Primer, Index 1 (100% over 27bp)
AAGTCGAACGGTAACAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA233120.1016720765003871RNA PCR Primer, Index 1 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph