FastQCFastQC Report
Thu 28 Sept 2023
SRR5742254.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5742254.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33925489
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCTGAGGCGTGATGACGAGGCTGGAATTCTCGGGTGCCAAGGAACTCC397995411.731456545843747RNA PCR Primer, Index 1 (100% over 27bp)
AAGGCTGAGGCGTGATGACGAGGCTGGAATTCTCGGGTGCCAAGGAACTC20803966.132250591877983RNA PCR Primer, Index 1 (100% over 26bp)
ATGCACCGAAGCTGCGGCAGCGACGCTGGAATTCTCGGGTGCCAAGGAAC9104902.683793297717831RNA PCR Primer, Index 1 (100% over 24bp)
AAGGCTGAGGCGTGATGACGAGGCACTGGAATTCTCGGGTGCCAAGGAAC5467871.6117291632848683RNA PCR Primer, Index 1 (100% over 24bp)
ACCGAAGCTGCGGCAGCGACGCTGGAATTCTCGGGTGCCAAGGAACTCCA5223911.5398186301750876RNA PCR Primer, Index 1 (100% over 28bp)
AATGAACCGTGAGGCTTAACCTGGAATTCTCGGGTGCCAAGGAACTCCAG4500401.326554202357997RNA PCR Primer, Index 1 (100% over 29bp)
AGGCTGAGGCGTGATGACGAGGCACTGGAATTCTCGGGTGCCAAGGAACT3961821.1678004110714513RNA PCR Primer, Index 1 (100% over 25bp)
AAACGATGTCGACTTGGAGGTTGTGCCTGGAATTCTCGGGTGCCAAGGAA3445121.0154960478241006RNA PCR Primer, Index 1 (100% over 23bp)
GATGTCGACTTGGAGGTTGTGCCTGGAATTCTCGGGTGCCAAGGAACTCC3315000.977141405389912RNA PCR Primer, Index 1 (100% over 27bp)
GATGACGAGGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCA3091070.9111349876194857RNA PCR Primer, Index 6 (100% over 37bp)
ACGGGAGACACACGGCGGGTGCTGGAATTCTCGGGTGCCAAGGAACTCCA2850140.8401175882829574RNA PCR Primer, Index 1 (100% over 28bp)
AGTCGAAGATACCAGCTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT2726560.8036906999336103RNA PCR Primer, Index 1 (100% over 30bp)
AAGCAGTGGGAGCACGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC2715760.800507252821028RNA PCR Primer, Index 1 (100% over 33bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGC2471890.7286232484371854RNA PCR Primer, Index 6 (100% over 50bp)
ACGGGAGACACACGGCGGGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG2247350.6624370248576226RNA PCR Primer, Index 1 (100% over 29bp)
ACGTACAAGCAGTGGGAGCACGCTGGAATTCTCGGGTGCCAAGGAACTCC2143400.6317963463990158RNA PCR Primer, Index 1 (100% over 27bp)
AGGCTGAGGCGTGATGACGAGGTGGAATTCTCGGGTGCCAAGGAACTCCA2025600.5970731917821435RNA PCR Primer, Index 1 (100% over 28bp)
ACAAGCAGTGGGAGCACGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1934360.570178958953252RNA PCR Primer, Index 1 (100% over 31bp)
GAGGCGTGATGACGAGGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGT1895370.558686125349586RNA PCR Primer, Index 1 (100% over 30bp)
ACCCCGAGGAAAAGAAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1736490.5118540811600387RNA PCR Primer, Index 1 (100% over 31bp)
ACGAGAGGACCGGAGTGGACGCTGGAATTCTCGGGTGCCAAGGAACTCCA1683900.49635246230349106RNA PCR Primer, Index 1 (100% over 28bp)
ATGCGAGAGTAGGGAACTGCCAGGCATGGAATTCTCGGGTGCCAAGGAAC1547520.4561525995984907RNA PCR Primer, Index 1 (100% over 24bp)
AATGAGGCGAACCGGGGGAACTGAAACTGGAATTCTCGGGTGCCAAGGAA1538400.45346435537008767RNA PCR Primer, Index 1 (100% over 23bp)
TGAGGCGAACCGGGGGAACTGATGGAATTCTCGGGTGCCAAGGAACTCCA1339560.3948535568639851RNA PCR Primer, Index 1 (100% over 28bp)
ATCTCCGGGGGTAGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1337890.3943613016160209RNA PCR Primer, Index 1 (100% over 33bp)
AGCGGCGAGCGAACGGGGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAG1269620.3742377891737979RNA PCR Primer, Index 1 (100% over 29bp)
ACGGTCCAGACTCCTACGGGAGGCTGGAATTCTCGGGTGCCAAGGAACTC1251310.3688406672634844RNA PCR Primer, Index 1 (100% over 26bp)
AGGGAACTGCCAGGCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG1242630.36628211902855695RNA PCR Primer, Index 6 (100% over 34bp)
AGCTCAGCTGGGAGAGCGCCTGCTGGAATTCTCGGGTGCCAAGGAACTCC1214230.3579108321769511RNA PCR Primer, Index 1 (100% over 27bp)
AAGTCGAACGGTAACAGGAAGAAGCTGGAATTCTCGGGTGCCAAGGAACT1168860.3445374066678891RNA PCR Primer, Index 1 (100% over 25bp)
AAAGAGAAGCGACCTCGCGAGAGCTGGAATTCTCGGGTGCCAAGGAACTC1161030.3422294075112668RNA PCR Primer, Index 1 (100% over 26bp)
AATGAACCGTGAGGCTTAACTGGAATTCTCGGGTGCCAAGGAACTCCAGT1159270.3417106235373645RNA PCR Primer, Index 1 (100% over 30bp)
ATTCATCTCCGGGGGTAGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAG1095420.322889966302328RNA PCR Primer, Index 1 (100% over 29bp)
AAGGCTGAGGCGTGATGACGAGGTGGAATTCTCGGGTGCCAAGGAACTCC1020420.3007826946871716RNA PCR Primer, Index 1 (100% over 27bp)
ACTGGAACTGAGACACGGTCCAGACTCCTGGAATTCTCGGGTGCCAAGGA1013790.2988284118763918RNA PCR Primer, Index 1 (100% over 22bp)
AAGTCGAACGGTAACAGGAAACAGCTGGAATTCTCGGGTGCCAAGGAACT962170.2836127137327335RNA PCR Primer, Index 1 (100% over 25bp)
GACTTGGAGGTTGTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC945140.2785928892579853RNA PCR Primer, Index 1 (100% over 33bp)
GTCGACTTGGAGGTTGTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT932570.2748877105352851RNA PCR Primer, Index 1 (100% over 30bp)
CGACTTGGAGGTTGTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA895600.2639902994471207RNA PCR Primer, Index 1 (100% over 32bp)
GAGGGGGGCTGCTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC845820.2493169663670876RNA PCR Primer, Index 6 (100% over 35bp)
ATCTCCGGGGGTAGAGCACTGTTTCGGCTGGAATTCTCGGGTGCCAAGGA845210.249137160557951RNA PCR Primer, Index 1 (100% over 22bp)
AGCGAAATTCCTTGTCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT835680.24632806324471845RNA PCR Primer, Index 1 (100% over 30bp)
AGGAAAAGCCTCTAAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC811100.23908277342737788RNA PCR Primer, Index 1 (100% over 33bp)
GAGAGGACCGGAGTGGACGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT802410.23652127755623506RNA PCR Primer, Index 1 (100% over 30bp)
GTGATGACGAGGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC758170.22348093493950819RNA PCR Primer, Index 6 (100% over 35bp)
GAGGCGAACCGGGGGAACTGAAACTGGAATTCTCGGGTGCCAAGGAACTC748360.2205893038122457RNA PCR Primer, Index 1 (100% over 26bp)
AGTCGAAGATACCAGCTGGCTGCTGGAATTCTCGGGTGCCAAGGAACTCC716110.2110831770177285RNA PCR Primer, Index 1 (100% over 27bp)
GAGAGTAGGGAACTGCCAGGCATGGAATTCTCGGGTGCCAAGGAACTCCA701130.2066676179671279RNA PCR Primer, Index 1 (100% over 28bp)
ACGATGTCGACTTGGAGGTTGTGCCTGGAATTCTCGGGTGCCAAGGAACT682930.2013029200551833RNA PCR Primer, Index 1 (100% over 25bp)
GATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAGGAACTC681610.20091383207475655RNA PCR Primer, Index 1 (100% over 26bp)
AGGTAGCGCCTCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG646790.19065016277289326RNA PCR Primer, Index 6 (100% over 34bp)
AGGGAACTGCCAGGCATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC631610.1861756509979856RNA PCR Primer, Index 1 (100% over 33bp)
GTACAAGCAGTGGGAGCACGCTGGAATTCTCGGGTGCCAAGGAACTCCAG619350.1825618489979614RNA PCR Primer, Index 1 (100% over 29bp)
ACGAGAGGACCGGAGTGGACGCATCTGGAATTCTCGGGTGCCAAGGAACT612890.18065767600284258RNA PCR Primer, Index 1 (100% over 25bp)
ACCCCGAGGAAAAGAAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC573740.16911768021973098RNA PCR Primer, Index 1 (100% over 33bp)
ATCACGGGAGACACACGGCGGGTGTGGAATTCTCGGGTGCCAAGGAACTC558270.16455768699457804RNA PCR Primer, Index 1 (100% over 26bp)
AAACGATGTCGACTTGGAGGTTGTGCCCTGGAATTCTCGGGTGCCAAGGA556450.16402121720338356RNA PCR Primer, Index 1 (100% over 22bp)
AGCTGGGAGAGCGCCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA542700.15996821740727157RNA PCR Primer, Index 1 (100% over 32bp)
GAACCGGGGGAACTGAAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC539720.15908982181509604RNA PCR Primer, Index 1 (100% over 31bp)
AGTGTACCCGCGGCAAGACGGTGGAATTCTCGGGTGCCAAGGAACTCCAG526890.1553080045507966RNA PCR Primer, Index 1 (100% over 29bp)
ACCCGCAGAAGAAGCACCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT525380.15486291148227813RNA PCR Primer, Index 1 (100% over 30bp)
AGCGGCGAGCGAACGGGGAGGAGCCCTGGAATTCTCGGGTGCCAAGGAAC525240.15482164457526318RNA PCR Primer, Index 1 (100% over 24bp)
GGGGGTAGAGCACTGTTTCGGCTGGAATTCTCGGGTGCCAAGGAACTCCA523640.15435002278080648RNA PCR Primer, Index 1 (100% over 28bp)
ACTGGAACTGAGACACGGTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG522090.15389313916742658RNA PCR Primer, Index 1 (100% over 29bp)
AGGCTGAGGCGTGATGACGAGGCATGGAATTCTCGGGTGCCAAGGAACTC520920.15354826573023014RNA PCR Primer, Index 1 (100% over 26bp)
ACTGTTTCGGCAAGGGGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT513100.15124321420982317RNA PCR Primer, Index 1 (100% over 30bp)
AATGAACCGTGAGGCTTAACCTTGGAATTCTCGGGTGCCAAGGAACTCCA513050.1512284760287464RNA PCR Primer, Index 1 (100% over 28bp)
CGGGGGTAGAGCACTGTTTCGGCTGGAATTCTCGGGTGCCAAGGAACTCC501180.14772963184112098RNA PCR Primer, Index 1 (100% over 27bp)
GAACCGTGAGGCTTAACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA496440.1463324522750431RNA PCR Primer, Index 1 (100% over 32bp)
GATGTCGACTTGGAGGTTGTGCTGGAATTCTCGGGTGCCAAGGAACTCCA488170.1438947571249452RNA PCR Primer, Index 1 (100% over 28bp)
CATGCGAGAGTAGGGAACTGCCAGGCATGGAATTCTCGGGTGCCAAGGAA486620.1434378735115653RNA PCR Primer, Index 1 (100% over 23bp)
AACGTCGCAAGACCAAAGAGGGGGATGGAATTCTCGGGTGCCAAGGAACT485010.14296330408089328RNA PCR Primer, Index 1 (100% over 25bp)
ATCACGGGAGACACACGGCGGGTGCTGGAATTCTCGGGTGCCAAGGAACT481440.14191099795201184RNA PCR Primer, Index 1 (100% over 25bp)
AAACGATGTCGACTTGGAGGTTGTGCTGGAATTCTCGGGTGCCAAGGAAC472950.13940845480517614RNA PCR Primer, Index 1 (100% over 24bp)
ACCCCGAGGAAAAGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG472490.13927286353926982RNA PCR Primer, Index 6 (100% over 34bp)
AACTGGAGGACCGAACCGACTTGGAATTCTCGGGTGCCAAGGAACTCCAG470500.13868628393241436RNA PCR Primer, Index 1 (100% over 29bp)
GAGGCGTGATGACGAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA464610.13695012620157074RNA PCR Primer, Index 1 (100% over 32bp)
TGAGGCGTGATGACGAGGCACTGGAATTCTCGGGTGCCAAGGAACTCCAG463150.136519771314129RNA PCR Primer, Index 1 (100% over 29bp)
GAACGGTAACAGGAAGAAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT445500.1313171933940289RNA PCR Primer, Index 1 (100% over 30bp)
AAGTCGAACGGTAACAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA443520.13073356142338877RNA PCR Primer, Index 1 (100% over 32bp)
AAGGCTGAGGCGTGATGACGAGGCATGGAATTCTCGGGTGCCAAGGAACT440490.12984042765013645RNA PCR Primer, Index 1 (100% over 25bp)
AACCGGTACTAATGAACCGTGAGGCTTAACCTGGAATTCTCGGGTGCCAA433700.12783898265991098No Hit
CTGCTTCCAGGAAAAGCCTCTAAGCTGGAATTCTCGGGTGCCAAGGAACT427370.12597312893559176RNA PCR Primer, Index 1 (100% over 25bp)
CGGTACTAATGAACCGTGAGGCTTAACCTGGAATTCTCGGGTGCCAAGGA427190.1259200714837154RNA PCR Primer, Index 1 (100% over 22bp)
AAGGTCCCAAAGTCATGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA420980.12408958939398045RNA PCR Primer, Index 1 (100% over 32bp)
ATCTCCGGGGGTAGAGCACTGTTTCGGTGGAATTCTCGGGTGCCAAGGAA419420.12362975814438518RNA PCR Primer, Index 1 (100% over 23bp)
AGGTGCGAAAGCGTGGGGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAG418660.12340573779201827RNA PCR Primer, Index 1 (100% over 29bp)
AGGGGTTCGAATCCCCTAGGGGACTGGAATTCTCGGGTGCCAAGGAACTC399510.11776101443961501RNA PCR Primer, Index 1 (100% over 26bp)
GAGAGGACCGGAGTGGACGCATCTGGAATTCTCGGGTGCCAAGGAACTCC397060.11703884356685323RNA PCR Primer, Index 1 (100% over 27bp)
AATTGGTAGAGCACCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC388750.11458935787189391RNA PCR Primer, Index 1 (100% over 33bp)
ACGGTCCAGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCC387860.11432701824872739RNA PCR Primer, Index 6 (100% over 36bp)
GAAAGCTATTTAGGTAGCGCCTCGTGATGGAATTCTCGGGTGCCAAGGAA387010.11407646917042229RNA PCR Primer, Index 1 (100% over 23bp)
AGTCCGGATTGGAGTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC386970.11406467862556086RNA PCR Primer, Index 1 (100% over 31bp)
AGGTGGTTAGAGCGCACCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT381000.1123049398049944RNA PCR Primer, Index 1 (100% over 30bp)
AAGTACCCCGAGGAAAAGAAATCTGGAATTCTCGGGTGCCAAGGAACTCC376140.11087238860433227RNA PCR Primer, Index 1 (100% over 27bp)
CATGCACCGAAGCTGCGGCAGCGACGCTGGAATTCTCGGGTGCCAAGGAA375040.11054814862064331RNA PCR Primer, Index 1 (100% over 23bp)
ACCGGAGAATGTTATCACGGGAGACTGGAATTCTCGGGTGCCAAGGAACT373720.11015906064021656RNA PCR Primer, Index 1 (100% over 25bp)
GGGAGACACACGGCGGGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC364450.10742660186858323RNA PCR Primer, Index 1 (100% over 31bp)
ACGGTCCAGACTCCTACGGGAGGCAGCTGGAATTCTCGGGTGCCAAGGAA362000.10670443099582146RNA PCR Primer, Index 1 (100% over 23bp)
ACGGTGGATGCCCTGGCAGTCTGGAATTCTCGGGTGCCAAGGAACTCCAG359020.10582603540364591RNA PCR Primer, Index 1 (100% over 29bp)
ATGCGAGAGTAGGGAACTGCCAGACATGGAATTCTCGGGTGCCAAGGAAC354580.10451728492402866RNA PCR Primer, Index 1 (100% over 24bp)
AGAGCACTGTTTCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG350640.1033559162551791RNA PCR Primer, Index 6 (100% over 34bp)
ATTCCTGTACTTGGTGTTACTGGAATTCTCGGGTGCCAAGGAACTCCAGT346440.10211790904473037RNA PCR Primer, Index 1 (100% over 30bp)
AGTTCAATTGGTAGAGCACCGGTGGAATTCTCGGGTGCCAAGGAACTCCA345620.1018762028750713RNA PCR Primer, Index 1 (100% over 28bp)
ATTTAGGTAGCGCCTCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGT344910.10166692070378115RNA PCR Primer, Index 1 (100% over 30bp)
ACTGGAGGACCGAACCGACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT344750.1016197585243355RNA PCR Primer, Index 1 (100% over 30bp)
AGCTCAGTTGGTAGAGCACGACCTGGAATTCTCGGGTGCCAAGGAACTCC344480.10154017234652093RNA PCR Primer, Index 1 (100% over 27bp)
AACGTCGCAAGACCAAAGAGGGGGTGGAATTCTCGGGTGCCAAGGAACTC343740.10132204726658472RNA PCR Primer, Index 1 (100% over 26bp)
AGAAGAAGCACCGGCTAACTCCGTGCTGGAATTCTCGGGTGCCAAGGAAC343110.10113634618501742RNA PCR Primer, Index 1 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph