FastQCFastQC Report
Mon 3 Jul 2023
SRR5766383_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766383_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15131686
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length53-70
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT4557593.0119512128390715No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT2504031.6548255098605666Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG1555171.027757250580008No Hit
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG1390760.9191044540575319Illumina Small RNA Adapter 2 (100% over 21bp)
CCAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA1376200.9094822612628891No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG1296520.8568245468482495No Hit
CCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG1171730.7743552172573499No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT1079620.7134829522632178No Hit
CCTAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCA874800.578124605546269No Hit
CCTCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAA863640.5707493533767486No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT811850.5365231607370123No Hit
CCTGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA764630.505317120643397No Hit
CCCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG640560.4233236137731116No Hit
CCCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAG639680.42274205267013865No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG513440.3393144689891133No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT462950.3059474007060416No Hit
CCAAATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCAA460760.3045001065975067No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT453470.29968240155128784No Hit
CCTGCTCTGCTAGGCGGACTACTTGCGTGCCTTGGAATTCTCGGGTGCCA394770.26088963252343456No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG387470.2560653188283183No Hit
CCCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTTGGAATTCTCGGGT338750.22386798140008984No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG331890.21933444825645998No Hit
CCAAGAGGTGCACAATCGACCGATCCTGGAATTCTCGGGTGCCAAGGAGA281520.18604668376015732RNA PCR Primer, Index 1 (95% over 22bp)
CCTCAATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCA274330.18129506520291264No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGTGGAATTCTCGGGTGCCAA272470.18006585650799256No Hit
CCTGCTCTGCTAGGCGGACTACTTGCGTGCCTGGAATTCTCGGGTGCCAA255260.1686923717555334No Hit
CCCCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAA248000.16389449265600675No Hit
CCTTGGGCCAGCATCAGTTTTGGTGGCAGGATTGGAATTCTCGGGTGCCA242830.16047782117604079No Hit
CCCCCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCA230340.1522236187031637No Hit
CCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCTGGAATTCT227430.15030050187401456No Hit
CCCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA220390.14564801305023115No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA202940.1341159207242339No Hit
CCGCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAA197480.13050759842624277No Hit
CCTTAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCC197390.130448120586166No Hit
CCGATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT196890.130117688141295No Hit
CCTCAAGAGGTGCACAATCGACCGATCCTGGAATTCTCGGGTGCCAAGGA181720.12009236776390946RNA PCR Primer, Index 1 (100% over 22bp)
CCCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT172250.11383397725805307No Hit
CCTTGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGC158700.10487925800204947No Hit
CCTTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG156670.10353770227587329No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG153530.10146258652208352No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)