FastQCFastQC Report
Mon 3 Jul 2023
SRR5766384_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766384_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14515649
Total Bases967.7 Mbp
Sequences flagged as poor quality0
Sequence length53-70
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT5153063.550003172438242No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT4131602.8463074575583907Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG1826681.2584211701454064No Hit
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG1571551.0826591356679953Illumina Small RNA Adapter 2 (100% over 21bp)
CCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG1206040.8308550310082587No Hit
CCAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA1189100.8191848673111344No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG1127860.7769959166138558No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT833230.5740218711543659No Hit
CCTAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCA782580.5391284950469661No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT695240.4789589497514028No Hit
CCTGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA683230.47068512058951No Hit
CCTCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAA655550.4516160455519419No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT577400.397777598507652No Hit
CCCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAG535760.369091316550848No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG531310.3660256596174239No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT505040.3479279500351655No Hit
CCAAATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCAA494960.34098372039720715No Hit
CCCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG447920.3085773154200684No Hit
CCTGCTCTGCTAGGCGGACTACTTGCGTGCCTTGGAATTCTCGGGTGCCA346410.2386458917544782No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG338070.23290036842307224No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG311100.21432042067151114No Hit
CCAAGAGGTGCACAATCGACCGATCCTGGAATTCTCGGGTGCCAAGGAGA286670.19749030856284827RNA PCR Primer, Index 1 (95% over 22bp)
CCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCTGGAATTCT277450.1911385429614618No Hit
CCTCAATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCA269600.18573058634856768No Hit
CCCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTTGGAATTCTCGGGT256850.17694696255055492No Hit
CCTTGGGCCAGCATCAGTTTTGGTGGCAGGATTGGAATTCTCGGGTGCCA252270.17379174709997466No Hit
CCCCCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCA243560.1677913264505087No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGTGGAATTCTCGGGTGCCAA243360.16765354411642222No Hit
CCTGCTCTGCTAGGCGGACTACTTGCGTGCCTGGAATTCTCGGGTGCCAA223970.15429554682673852No Hit
CCCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA201260.1386503627912193No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG190010.13090010649885514No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG178710.12311540462296931No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA174400.12014619532340579No Hit
CCCCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAA170900.11773500447689249No Hit
CCATTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT162260.11178280764435677No Hit
CCTGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCTGGAATTCTCGGGT161220.11106633950710712No Hit
CCGATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT161030.11093544628972497No Hit
CCTTAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCC158640.1092889473973916No Hit
CCTGGCATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT156950.10812468667436088Illumina Paired End PCR Primer 2 (100% over 27bp)
CCCAAGAGGTGCACAATCGACCGATCCTGGAATTCTCGGGTGCCAAGGAG156800.10802134992379604RNA PCR Primer, Index 1 (95% over 22bp)
CCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCCTGGAATTC154310.10630595986441942No Hit
CCGCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAA149160.1027580647616927No Hit
CCTCAAGAGGTGCACAATCGACCGATCCTGGAATTCTCGGGTGCCAAGGA145900.10051221271608318RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)