FastQCFastQC Report
Mon 3 Jul 2023
SRR5766385_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766385_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14953283
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length53-70
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT9093036.080958943932246Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT3199172.1394432246082684No Hit
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG2409161.611124460093479Illumina Small RNA Adapter 2 (100% over 21bp)
CCAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA1517151.014593250191279No Hit
CCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG1439170.9624441669431388No Hit
CCTCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAA1437840.9615547301552442No Hit
CCCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAG1033260.6909920717744725No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG1008370.6743468976010151No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT1002030.6701070259955623No Hit
CCTGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA913310.6107755734978065No Hit
CCTAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCA892060.5965646473754291No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG872780.5836711576982794No Hit
CCAAATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCAA750990.5022241604067816No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT677400.4530108873081583No Hit
CCTGCTCTGCTAGGCGGACTACTTGCGTGCCTTGGAATTCTCGGGTGCCA506170.33850091648770375No Hit
CCCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG502650.3361469183723735No Hit
CCTGCTCTGCTAGGCGGACTACTTGCGTGCCTGGAATTCTCGGGTGCCAA423540.2832421482292551No Hit
CCTCAATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCA379690.2539174842073142No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT366560.24513680373734653No Hit
CCCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTTGGAATTCTCGGGT364650.243859492259994No Hit
CCCCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAA362160.2421943060931837No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT361950.2420538687056214No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGTGGAATTCTCGGGTGCCAA349860.23396868767881943No Hit
CCTGGCATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT335440.22432532039954037Illumina Paired End PCR Primer 2 (100% over 27bp)
CCAAGAGGTGCACAATCGACCGATCCTGGAATTCTCGGGTGCCAAGGAGA308440.20626908485581394RNA PCR Primer, Index 1 (95% over 22bp)
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG304700.20376796185827556No Hit
CCGCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAA295690.1977425291823876No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG280890.18784503710656716No Hit
CCTCAAGAGGTGCACAATCGACCGATCCTGGAATTCTCGGGTGCCAAGGA274980.18389272777088483RNA PCR Primer, Index 1 (100% over 22bp)
CCCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA271760.1817393544949293No Hit
CCCCCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCA263990.17654317115512358No Hit
CCCAAGAGGTGCACAATCGACCGATCCTGGAATTCTCGGGTGCCAAGGAG242270.16201793278439255RNA PCR Primer, Index 1 (95% over 22bp)
CCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA231880.1550696258473808No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG227740.15230100306400943No Hit
CCCTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT227160.1519131283745516No Hit
CCCAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG218760.14629563287205893Illumina Small RNA Adapter 2 (100% over 21bp)
CCAAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAG213320.14265763578473037No Hit
CCTTGGGCCAGCATCAGTTTTGGTGGCAGGATTGGAATTCTCGGGTGCCA202340.13531476666361494No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGTGGTTCAGCAGGAAT194770.13025233321672572Illumina Paired End PCR Primer 2 (96% over 27bp)
CCTTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG193090.1291288341162272No Hit
CCTTAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCC183410.12265533929906897No Hit
CCTTGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGC176000.11769990576651293No Hit
CCTGGCCTTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT168460.1126575348035612Illumina Paired End PCR Primer 2 (100% over 27bp)
CCAGTTTCTTTACTTATTCAATGAAGCGGTGGAATTCTCGGGTGCCAAGG167000.11168116058527082Illumina Small RNA Adapter 2 (100% over 21bp)
CCCCGCGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG154720.10346891716019821Illumina Small RNA Adapter 2 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)