FastQCFastQC Report
Mon 3 Jul 2023
SRR5766387_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766387_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12164770
Total Bases812.7 Mbp
Sequences flagged as poor quality0
Sequence length54-70
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT2581142.121815702228649No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT1582301.3007233182378295Illumina Paired End PCR Primer 2 (100% over 27bp)
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG1135620.9335318300304898Illumina Small RNA Adapter 2 (100% over 21bp)
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT998640.8209279747993591No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG976350.8026045704111134No Hit
CCAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA945870.7775486096325701No Hit
CCTCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAA921690.757671538385025No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG880900.7241402837867054No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT780220.6413766968056116No Hit
CCTAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCA680470.559377612564808No Hit
CCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG665340.5469400572308395No Hit
CCCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAG574650.4723887093631857No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG547950.4504400823032413No Hit
CCTGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA481330.39567538062782937No Hit
CCCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG464450.38179924486858363No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGTGGAATTCTCGGGTGCCAA456830.37553525467394777No Hit
CCCCCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCA397600.32684547262299246No Hit
CCTGCTCTGCTAGGCGGACTACTTGCGTGCCTTGGAATTCTCGGGTGCCA369690.3039021699547135No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA347050.2852910494814123No Hit
CCAAATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCAA344100.2828660139073735No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT331650.27263154173897247No Hit
CCTTGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGC286960.23589430790717786No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG281210.23116754365269543No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT273440.22478024656446444No Hit
CCCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTTGGAATTCTCGGGT254200.20896408234598765No Hit
CCTCAATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCA227630.18712232126049239No Hit
CCCCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAA217560.17884431847046842No Hit
CCGCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAA206800.16999910396990656No Hit
CCTGCTCTGCTAGGCGGACTACTTGCGTGCCTGGAATTCTCGGGTGCCAA202350.16634099945991582No Hit
CCCTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT195550.16075108695026702No Hit
CCTTGGGCCAGCATCAGTTTTGGTGGCAGGATTGGAATTCTCGGGTGCCA181200.14895472746299354No Hit
CCCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA174580.143512783225659No Hit
CCCCCCACTAATAGGGAACGTGAGCTGGGTTTATGGAATTCTCGGGTGCC166640.13698573832468677No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCC163260.13420722298900845No Hit
CCTTAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCC161250.13255491061483282No Hit
CCGATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT158030.12990792263232268No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGC156680.12879816058996593No Hit
CCTCAAGAGGTGCACAATCGACCGATCCTGGAATTCTCGGGTGCCAAGGA147330.1211120308891989RNA PCR Primer, Index 1 (100% over 22bp)
CCTTGCCAAGGACGTTTTCATTAATCAAGAACGAATGGAATTCTCGGGTG145680.11975565505965176No Hit
CCCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT144670.11892538864277746No Hit
CCACTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAG133160.10946363967423962No Hit
CCTTGTGAAACTCCGTCGTGCTGGGGATAGAGCATTGGAATTCTCGGGTG130400.10719479283208806No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATGGAATTCTCGGGTG128310.10547671678132838No Hit
CCAAGAGGTGCACAATCGACCGATCCTGGAATTCTCGGGTGCCAAGGAGA127890.10513145747926182RNA PCR Primer, Index 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)