FastQCFastQC Report
Mon 3 Jul 2023
SRR5766395_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766395_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12562086
Total Bases844.1 Mbp
Sequences flagged as poor quality0
Sequence length54-70
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT2009401.5995751024153155No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT1987141.5818551154641036Illumina Paired End PCR Primer 2 (100% over 27bp)
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG1829201.456127589000744Illumina Small RNA Adapter 2 (100% over 21bp)
CCAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA1174960.9353223660465309No Hit
CCTCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAA1077690.8578909585557685No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT1012700.8061559202826665No Hit
CCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG860280.6848225684810628No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG726010.5779374540183851No Hit
CCTAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCA703690.5601697042991108No Hit
CCCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAG678660.5402446695556773No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGTGGAATTCTCGGGTGCCAA614600.48924995418754497No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG606090.4824756015840044No Hit
CCTGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA549640.43753879729847417No Hit
CCCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTTGGAATTCTCGGGT442730.35243350507232635No Hit
CCTGCTCTGCTAGGCGGACTACTTGCGTGCCTTGGAATTCTCGGGTGCCA423000.3367275148410861No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA391610.31173962668302063No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT384800.30631855250791945No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG325780.2593359096570426No Hit
CCTTGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGC320850.25541140221456854No Hit
CCAAATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCAA301950.2403661302748604No Hit
CCCCCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCA299190.23816904294398242No Hit
CCCTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT288570.22971503299690832No Hit
CCTGCTCTGCTAGGCGGACTACTTGCGTGCCTGGAATTCTCGGGTGCCAA285330.22713584352152977No Hit
CCCCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAA284230.2262601927737161No Hit
CCAAGAGGTGCACAATCGACCGATCCTGGAATTCTCGGGTGCCAAGGAGA253500.20179769506433887RNA PCR Primer, Index 1 (95% over 22bp)
CCTCAATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCA246710.1963925418119252No Hit
CCGCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAA242970.19341532926935862No Hit
CCTCAAGAGGTGCACAATCGACCGATCCTGGAATTCTCGGGTGCCAAGGA236970.18863905246310206RNA PCR Primer, Index 1 (100% over 22bp)
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT234670.18680814635403706No Hit
CCCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG221460.1762923769189289No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT205160.16331682492859864No Hit
CCACTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAG184380.1467749862562635No Hit
CCCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA183230.145859533201731No Hit
CCCAAGAGGTGCACAATCGACCGATCCTGGAATTCTCGGGTGCCAAGGAG180930.14402862709266598RNA PCR Primer, Index 1 (95% over 22bp)
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG166060.13219142107449353No Hit
CCTTGCCAAGGACGTTTTCATTAATCAAGAACGAATGGAATTCTCGGGTG159430.12691363520358004No Hit
CCTTGCCAAGGACGTTTTCATTAATCAAGAACGTGGAATTCTCGGGTGCC157580.12544094985498427No Hit
CCTTAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCC151560.1206487521260402No Hit
CCTCTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGG150710.11997211291182054No Hit
CCAAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAG150070.11946264338581983No Hit
CCTTGGGCCAGCATCAGTTTTGGTGGCAGGATTGGAATTCTCGGGTGCCA143080.11389828090653098No Hit
CCAGTTTCTTTACTTATTCAATGAAGCGGTGGAATTCTCGGGTGCCAAGG133920.10660649831564599Illumina Small RNA Adapter 2 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)