FastQCFastQC Report
Mon 3 Jul 2023
SRR5766397_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766397_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36194161
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length36-70
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG6903821.9074402636381045Illumina Small RNA Adapter 2 (100% over 21bp)
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT3941351.0889463634755894No Hit
CCCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAG3709821.0249774818650996No Hit
CCAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA3301140.9120642415222721No Hit
GCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT2728270.7537873305033925No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT2674860.7390308066541451No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG2123120.5865918538628372No Hit
CCTAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCA1977260.5462925359700975No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA1942080.5365727361382959No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG1863460.5148510004141276No Hit
GCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG1747890.4829204357023223No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGTGGAATTCTCGGGTGCCAA1725520.4767398807780073No Hit
CCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG1626070.4492630731238666No Hit
CCTTGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGC1360480.37588383385927915No Hit
CCCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTTGGAATTCTCGGGT1338000.3696728872925111No Hit
GCAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA1270170.35093229540532794No Hit
CCCTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT1253150.34622987945486566No Hit
GCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG1196460.33056713208520017No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG1060150.29290636133270226No Hit
GCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT1036850.2864688588858297No Hit
GCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG988930.27322915428264793Illumina Small RNA Adapter 2 (100% over 21bp)
CCTCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAA856880.23674536895605894No Hit
GCTAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCA809790.2237349831095684No Hit
CCCCCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCA807880.22320727368152007No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG802490.2217180832013208No Hit
CCTTGCCAAGGACGTTTTCATTAATCAAGAACGAATGGAATTCTCGGGTG748930.2069201162032738No Hit
GCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG744200.20561327557779277No Hit
GCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG666130.18404349806588968No Hit
GCTTGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGC629350.17388163798022557No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGGT624540.17255269434205148No Hit
GCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG547080.15115145230193344No Hit
GCTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGGT515980.1425589061174812No Hit
CCTTGCCAAGGACGTTTTCATTAATCAAGAACGTGGAATTCTCGGGTGCC487360.13465155332651582No Hit
CCAAATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCAA484720.13392215390764273No Hit
CCTGCTCTGCTAGGCGGACTACTTGCGTGCCTTGGAATTCTCGGGTGCCA468150.1293440674035793No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT467600.12919210919131405Illumina Paired End PCR Primer 2 (100% over 27bp)
CCGCTCTGCTAGGCGGACTACTTGCGTGCCTTGGAATTCTCGGGTGCCAA432340.11945020634681933No Hit
CCACTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAG429140.11856608583909432No Hit
CCAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGA424590.11730897699217285RNA PCR Primer, Index 1 (100% over 22bp)
CCCCCCACTAATAGGGAACGTGAGCTGGGTTTATGGAATTCTCGGGTGCC410380.11338292936255658No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTGC404430.11173901779350542No Hit
GCCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAG394550.10900929572590452No Hit
ACAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG386630.10682109746928516Illumina Small RNA Adapter 2 (100% over 21bp)
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT386430.10676583993755236No Hit
CCTGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCC378030.10444502360477427No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG370700.1024198350667667No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)