FastQCFastQC Report
Mon 3 Jul 2023
SRR5766399_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766399_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18922228
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length43-70
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG3975632.101036939201874Illumina Small RNA Adapter 2 (100% over 21bp)
GCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG2783201.470862733500516Illumina Small RNA Adapter 2 (100% over 21bp)
CCCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAG1795360.9488100449904736No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT1523090.8049210695484696No Hit
GCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT1308500.6915147624264966No Hit
CCAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA1298690.6863303835045217No Hit
GCAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA992250.5243832808694621No Hit
CCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG877550.4637667403648239No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG855290.45200279797918086No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT845700.4469346844356807No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG794330.4197867185618945No Hit
GCCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAG793000.4190838415011171No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA767420.40556534885849593No Hit
GCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT752430.3976434487524408No Hit
CCTAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCA706560.37340211734051615No Hit
GCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG689770.3645289550469427No Hit
GCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG682710.3607978933558987No Hit
GCTAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCA675340.3569030031770043No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGTGGAATTCTCGGGTGCCAA673580.35597288014920864No Hit
GCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG647100.3419787564128283No Hit
CCCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTTGGAATTCTCGGGT573840.3032623853808336No Hit
CCTTGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGC447270.23637279922850524No Hit
CCTCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAA412180.21782847136182906No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG393460.20793534461163876No Hit
GCTTGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGC367930.1944432759186709No Hit
CCAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGA359620.19005161548629473RNA PCR Primer, Index 1 (100% over 22bp)
CCCTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT342800.18116259882292932No Hit
GCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG340600.17999994503818473No Hit
GCTCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAA322370.17036577299459663No Hit
GCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA313760.16581556886430077No Hit
CCTGCTCTGCTAGGCGGACTACTTGCGTGCCTTGGAATTCTCGGGTGCCA301170.15916201834160332No Hit
CCCCCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCA300830.15898233548396096No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG295080.1559435812738331No Hit
CCAAATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCAA294160.15545738060021264No Hit
CCAAGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGA283830.14999819260184372RNA PCR Primer, Index 1 (100% over 22bp)
GCGCCAAGGACGTTTTCATTAATCAAGAACGTGGAATTCTCGGGTGCCAA273610.14459713729271206No Hit
GCAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGA262200.13856719198183215RNA PCR Primer, Index 1 (100% over 22bp)
GCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG249880.13205633078726248No Hit
GCCTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT237720.1256300262315833No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGGT233690.12350025588952844No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT228740.12088428487385312Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTTGCCAAGGACGTTTTCATTAATCAAGAACGAATGGAATTCTCGGGTG228640.12083143697454654No Hit
CCGCTCTGCTAGGCGGACTACTTGCGTGCCTTGGAATTCTCGGGTGCCAA226310.11960008092070341No Hit
GCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT204900.10828534567916633Illumina Paired End PCR Primer 2 (100% over 27bp)
GCAAGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGA203780.10769344920693272RNA PCR Primer, Index 1 (100% over 22bp)
GCAAATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCAA202010.10675804138920639No Hit
GCTGCTCTGCTAGGCGGACTACTTGCGTGCCTTGGAATTCTCGGGTGCCA201360.10641453004371366No Hit
CCACTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAG190170.10050085011130824No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)