FastQCFastQC Report
Mon 3 Jul 2023
SRR5766400_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766400_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17319070
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length44-70
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT3614142.0867979631700777No Hit
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG2433991.4053814667877662Illumina Small RNA Adapter 2 (100% over 21bp)
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT1707940.9861614971242683No Hit
CCAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA1702070.9827721696372842No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG1536130.8869587108314708No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG1495490.8634932476166446No Hit
GCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT1119090.6461605617391697No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG1043110.6022898458173562No Hit
CCTAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCA1020660.5893272560247174No Hit
CCCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAG1015190.5861688878213437No Hit
CCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG732150.4227420987385581No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA660690.38148122272154333No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG641690.3705106567500449No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGTGGAATTCTCGGGTGCCAA634420.36631297177042416No Hit
GCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT633270.365648963830044No Hit
GCAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA612020.3533792518882365No Hit
CCCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTTGGAATTCTCGGGT554470.32014998495877667No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGGT468710.2706323145526867No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT461460.26644617753724653Illumina Paired End PCR Primer 2 (100% over 27bp)
CCCTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT450630.26019295493349237No Hit
GCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG448860.2591709601035159No Hit
GCTAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCA399330.2305724268104465No Hit
GCTTGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGC369650.21343524796654786No Hit
GCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG360980.20842920549429042Illumina Small RNA Adapter 2 (100% over 21bp)
GCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG327120.18887850213666207No Hit
CCTCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAA324420.18731952697229123No Hit
GCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG312240.1802868167863517No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT309440.17867010180107823No Hit
CCCCCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCA303450.17521148652901108No Hit
GCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG288430.16653896542943705No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT285120.16462777735756018No Hit
CCTTGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGC281110.162312410539365No Hit
CCTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGTG272510.15734678594173937No Hit
GCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA270360.15610537979233297No Hit
GCGCCAAGGACGTTTTCATTAATCAAGAACGTGGAATTCTCGGGTGCCAA261260.1508510560901942No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTGC250410.14458628552225958No Hit
CCTGCTCTGCTAGGCGGACTACTTGCGTGCCTTGGAATTCTCGGGTGCCA238290.1375882192288616No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT236320.1364507447570799No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATGGAATTCTCGGGTG235600.13603501804658102No Hit
CCAAATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCAA233300.13470700216582068No Hit
GCTTGCCAAGGACGTTTTCATTAATCAAGAACGAATGGAATTCTCGGGTG224930.12987417915627109No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCC224840.12982221331745875No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG218520.12617305663641293No Hit
CCTCAATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCA215430.12438889617052186No Hit
CCCCCCACTAATAGGGAACGTGAGCTGGGTTTATGGAATTCTCGGGTGCC197070.11378786505280018No Hit
GCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG195840.11307766525569792No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGC191300.11045627738671882No Hit
CCATTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT183230.10579667383987708No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)