FastQCFastQC Report
Mon 3 Jul 2023
SRR5766402_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766402_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19558832
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length43-70
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG1872640.9574395853494727Illumina Small RNA Adapter 2 (100% over 21bp)
GCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT1604790.8204937799966788No Hit
GCAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA1348610.6895145886012007No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT1302850.6661185085080745No Hit
CCAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA1285690.6573449784731521No Hit
GCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG1201500.6143004858367821No Hit
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG1029310.5262635314828615Illumina Small RNA Adapter 2 (100% over 21bp)
GCCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAG926210.4735507723569587No Hit
GCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT867250.4434058230061999No Hit
GCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG847320.43321605298312293No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT772900.395166746153349No Hit
CCCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAG768410.39287110804980585No Hit
GCTAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCA729440.37294660540056784No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG661160.33803654533154126No Hit
GCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG655430.3351069225401599No Hit
CCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG626740.3204383574642903No Hit
TCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT589510.3014034784899221No Hit
CCTAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCA562000.28733822142344695No Hit
GCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG512190.26187146553536533No Hit
TCAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA505480.2584407903294021No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG476790.24377222525353254No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA460070.23522365752719795No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGTGGAATTCTCGGGTGCCAA438280.22408291047236356No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG411300.21028863073214188No Hit
GCCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTTGGAATTCTCGGGT409390.20931208980168142No Hit
CCCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTTGGAATTCTCGGGT399050.20402547554986922No Hit
TCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG380260.1944185624172241Illumina Small RNA Adapter 2 (100% over 21bp)
TCTAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCA367560.18792533214662308No Hit
ACAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA347670.17775601324250856No Hit
GCTCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAA345270.1765289461047572No Hit
TCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT324590.1659557176011328No Hit
GCCTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT314900.16100143403246164No Hit
ACTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT312080.1595596301456038No Hit
TCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG305530.15621075941549065No Hit
GCTTGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGC300570.15367482066413782No Hit
CCCTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT285030.14572956094719766No Hit
GCTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGGT282630.1445024938094463No Hit
GCTGCTCTGCTAGGCGGACTACTTGCGTGCCTTGGAATTCTCGGGTGCCA275420.14081617961645154No Hit
GCGCCAAGGACGTTTTCATTAATCAAGAACGTGGAATTCTCGGGTGCCAA271980.13905738338567455No Hit
GCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA266780.1363987379205466No Hit
CCTTGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGC264000.13497738515265123No Hit
GCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG246380.1259686672496599No Hit
GCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT238780.12208295464678054No Hit
GCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT237230.12129047378698278Illumina Paired End PCR Primer 2 (100% over 27bp)
CCATGTACACGGAGTCGAGCTCAACCCGCAACGCGATGGAATTCTCGGGT229830.11750701677891605No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGGT219440.11219483862840071No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG202350.1034570980516628No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)