FastQCFastQC Report
Mon 3 Jul 2023
SRR5766403_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766403_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22620433
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length43-70
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG2977711.316380636922379Illumina Small RNA Adapter 2 (100% over 21bp)
CCAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA2593041.1463264208956567No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT2538551.1222375805096214No Hit
CCCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAG1835740.8115406102084783No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG1563570.6912201901705418No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT1338100.5915448214452836No Hit
CCTAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCA1225700.5418552332751544No Hit
GCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT1214710.5369967940047832No Hit
GCAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA1167990.5163429011283736No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG1052790.46541549403585686No Hit
CCCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTTGGAATTCTCGGGT998130.44125150035810545No Hit
CCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG938960.41509373405893685No Hit
GCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG897280.39666791524282496No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG825500.3649355430110467No Hit
GCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG766340.33878219749374383Illumina Small RNA Adapter 2 (100% over 21bp)
GCTAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCA747160.330303137875389No Hit
GCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT650510.2875762811436899No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGTGGAATTCTCGGGTGCCAA633990.28027314950160326No Hit
CCCTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT618760.27354029872018804No Hit
CCTGCTCTGCTAGGCGGACTACTTGCGTGCCTTGGAATTCTCGGGTGCCA588790.2602912154687755No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA560260.2476787248060194No Hit
CCTCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAA538460.2380414203388591No Hit
GCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG536080.23698927425482968No Hit
GCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG462830.20460704708879798No Hit
CCTTGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGC419740.18555789802962658No Hit
GCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG394150.17424511723537742No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG387900.17148212856933376No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGGT354560.15674324182919044No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT354140.1565575689908323No Hit
CCCCCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCA349750.15461684575180323No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT348410.1540244609818035Illumina Paired End PCR Primer 2 (100% over 27bp)
CCAAATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCAA327720.14487786330173255No Hit
GCTTGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGC297980.13173045803323039No Hit
CCTCAATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCA274020.12113826468308543No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG267260.1181498161418926No Hit
CCGCTCTGCTAGGCGGACTACTTGCGTGCCTTGGAATTCTCGGGTGCCAA267240.11814097457816125No Hit
GCCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAG258000.11405617213428232No Hit
CCCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTGGAATTCTCGGGTG233700.10331367220070456No Hit
CCTTGCCAAGGACGTTTTCATTAATCAAGAACGAATGGAATTCTCGGGTG230390.10185039340316784No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)