FastQCFastQC Report
Mon 3 Jul 2023
SRR5766404_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766404_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21226232
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length47-70
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTTGGAATTCTCGGGT2000160.9423057281198095No Hit
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG1483780.6990312741328749Illumina Small RNA Adapter 2 (100% over 21bp)
CCAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA1434080.6756168499430327No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG1256890.5921399521120847No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT1163940.5483497966101567No Hit
CCCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAG1088060.5126015771428486No Hit
CCCTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT1035260.4877266959109841No Hit
CCTAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCA806280.379850743174766No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA791060.37268037021361117No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT786690.3706215968995345No Hit
CCTTGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGC676350.318638748507036No Hit
GCAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA568110.26764524198171397No Hit
CCCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGGAATTCTCGGG566450.26686319079146975No Hit
CCCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTGGAATTCTCGGGTG531160.2502375362711573No Hit
GCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT523440.2466005271213468No Hit
GCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG507520.2391003735377998No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG492000.23178866602419118No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG458810.2161523533710552No Hit
CCTGCTCTGCTAGGCGGACTACTTGCGTGCCTTGGAATTCTCGGGTGCCA444850.20957558553020622No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGTGGAATTCTCGGGTGCCAA439710.20715405353149818No Hit
CCTTGCCAAGGACGTTTTCATTAATCAAGAACGAATGGAATTCTCGGGTG414220.19514532772467577No Hit
CCTCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAA394480.18584551417321737No Hit
GCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT347890.1638962581771461No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT346650.16331207536033715No Hit
GCTAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCA341750.16100361100359215No Hit
CCCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCTGGAATTCTCGG341170.16073036420218154No Hit
GCCTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT303180.14283269870978513No Hit
CCCGGGGCCTTTCCTTCTGGCTAACCTTGAGTTGGAATTCTCGGGTGCCA294320.1386586182606503No Hit
CCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG281040.13240220873869654No Hit
CCTCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTTGGAATTCTCGGGT261780.12332853047116417No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT258080.12158540432423429Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG237250.11177207523219382No Hit
CCCCAACGGGGCCTTTCCTTCTGGCTAACCTTGGAATTCTCGGGTGCCAA227880.10735772604388757No Hit
GCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG213020.10035695454567725No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG212820.10026273151070807No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)