FastQCFastQC Report
Mon 3 Jul 2023
SRR5766405_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766405_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19918696
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length39-70
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG2250311.129747650147379Illumina Small RNA Adapter 2 (100% over 21bp)
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT1936550.9722272984135105No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT1541200.7737454299217178No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG1465470.7357258728181805No Hit
GCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT1330340.6678850864534506No Hit
CCAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA1297900.6515988797660248No Hit
GCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT1293410.6493447161400525No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA1265460.6353126730785992No Hit
GCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG1242830.6239514875873401Illumina Small RNA Adapter 2 (100% over 21bp)
CCCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTTGGAATTCTCGGGT1124420.5645048250146495No Hit
CCCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAG1090830.5476412712960728No Hit
CCTTGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGC1074710.5395483720420252No Hit
CCTAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCA992370.4982103246116111No Hit
CCCTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT973460.4887167312559015No Hit
GCAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA947600.47573395366845306No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG852980.4282308440271392No Hit
GCTAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCA833050.4182251689568433No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGTGGAATTCTCGGGTGCCAA709780.35633858762641896No Hit
GCCTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT650470.3265625420459251No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG634060.3183240509318481No Hit
GCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG611200.3068473960343589No Hit
GCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG519000.26055922536294546No Hit
CCTTGCCAAGGACGTTTTCATTAATCAAGAACGAATGGAATTCTCGGGTG493460.24773710086242592No Hit
GCTTGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGC486310.24414750845135644No Hit
GCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG437360.21957260656018848No Hit
GCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA404940.20329644069069583No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG378600.19007268347285383No Hit
CCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG350730.17608080368313267No Hit
CCTTGCCAAGGACGTTTTCATTAATCAAGAACGTGGAATTCTCGGGTGCC343560.17248117045413014No Hit
CCTGCTCTGCTAGGCGGACTACTTGCGTGCCTTGGAATTCTCGGGTGCCA301010.15111933030154182No Hit
GCCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAG299950.15058716695108956No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT294450.14782594201949767Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGGT290600.1458930845673833No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT286080.14362385971451141No Hit
CCCCCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCA283100.14212777784248526No Hit
GCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG264990.1330358172040981No Hit
GCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG258050.12955165338132577No Hit
CCTCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAA249350.12518389758044401No Hit
CCCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGGAATTCTCGGG236050.11850675365495814No Hit
CCCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTGGAATTCTCGGGTG231190.1160668348972242No Hit
GCGCCAAGGACGTTTTCATTAATCAAGAACGTGGAATTCTCGGGTGCCAA229680.11530875314327806No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG228260.11459585507003069No Hit
CCCCCCACTAATAGGGAACGTGAGCTGGGTTTATGGAATTCTCGGGTGCC227290.11410887540027721No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCC225920.1134210793718625No Hit
GCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT223180.11204548731503308Illumina Paired End PCR Primer 2 (100% over 27bp)
GCTTGCCAAGGACGTTTTCATTAATCAAGAACGAATGGAATTCTCGGGTG220410.11065483403130405No Hit
GCTCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAA213090.10697989466780355No Hit
CCAACTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAA207290.10406805746721573No Hit
CCTGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCC201650.10123654681009238No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)