FastQCFastQC Report
Mon 3 Jul 2023
SRR5766408_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766408_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23532573
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length38-70
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG3015621.2814663317946575Illumina Small RNA Adapter 2 (100% over 21bp)
GCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG2211220.9396422567137049Illumina Small RNA Adapter 2 (100% over 21bp)
GCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT2010920.8545261922697531No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT1913800.8132557370585868No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT1900630.8076592389620973No Hit
GCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT1834350.779494023029271No Hit
CCAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA1774760.7541716751500144No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT1720590.731152517831348Illumina Paired End PCR Primer 2 (100% over 27bp)
GCAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA1559570.6627282108080574No Hit
GCCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAG1514060.6433890590714411No Hit
CCCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAG1496010.6357188395846047No Hit
GCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT1432030.6085309923398516Illumina Paired End PCR Primer 2 (100% over 27bp)
CCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG1248520.5305497193188352No Hit
GCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG1030880.4380651448526262No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGTGGAATTCTCGGGTGCCAA1005730.42737783071999824No Hit
GCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG934380.39705815424433183No Hit
CCTAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCA930750.39551561148880743No Hit
GCTAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCA919700.39081999235697684No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA909590.38652381955853277No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG869190.3693561260810707No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG675310.28696819510556704No Hit
CCCTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT675160.2869044536693884No Hit
GCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG668200.2839468510306969No Hit
CCCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTTGGAATTCTCGGGT661950.28129095785658453No Hit
GCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG557850.2370544011485697No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG529190.22487553740936023No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG521460.2215907287316181No Hit
CCTCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAA519850.22090657064996674No Hit
GCCTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT490200.20830701343197786No Hit
GCTCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAA454230.19302181703632662No Hit
CCTTGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGC424140.18023528493888025No Hit
GCTGCTCTGCTAGGCGGACTACTTGCGTGCCTTGGAATTCTCGGGTGCCA396320.16841337324227146No Hit
CCAAATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCAA379020.16106186093632857No Hit
CCCCCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCA363050.15427552269783673No Hit
GCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG359990.15297519739979135No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG345180.1466817929344148No Hit
GCTTGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGC337420.14338423596943692No Hit
GCGCCAAGGACGTTTTCATTAATCAAGAACGTGGAATTCTCGGGTGCCAA302200.12841774675467915No Hit
CCGCTCTGCTAGGCGGACTACTTGCGTGCCTTGGAATTCTCGGGTGCCAA285490.12131695076437242No Hit
CCTGCTCTGCTAGGCGGACTACTTGCGTGCCTTGGAATTCTCGGGTGCCA284600.12093875157637883No Hit
GCTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGGT277770.11803639151570888No Hit
GCAAATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCAA276760.11760719917877233No Hit
GCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA272820.11593292412181193No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGGT257420.10938880334079916No Hit
CCTTGCCAAGGACGTTTTCATTAATCAAGAACGAATGGAATTCTCGGGTG255890.10873864069177645No Hit
GCTGCTCTGCTAGGCGGACTACTTGCGTGCCTGGAATTCTCGGGTGCCAA255420.10853891752508321No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATGGAATTCTCGGGTG247990.10538159171969849No Hit
ACAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG237580.10095793604889698Illumina Small RNA Adapter 2 (100% over 21bp)
GCTTTTGATGATTCATAATAACTTTTCGAATCGCATGGAATTCTCGGGTG235680.10015054452396685No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)