FastQCFastQC Report
Tue 4 Jul 2023
SRR5766409_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766409_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22997566
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length30-70
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT5272632.292690452546152No Hit
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG3235651.4069532401820262Illumina Small RNA Adapter 2 (100% over 21bp)
GCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT2684991.1675105095904499No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG2638411.1472561922422573No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG2094290.910657240857576No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT2031990.8835674175258373Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT1854090.806211405154789No Hit
CCAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA1648480.7168062915875532No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG1548830.6734756191155186No Hit
CCTAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCA1484480.6454943971027195No Hit
CCCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAG905380.393685140418773No Hit
GCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG875350.3806272368128001No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTGGAATTCTC813110.3535635031985559No Hit
GCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT799730.3477454961972932Illumina Paired End PCR Primer 2 (100% over 27bp)
GCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG793970.34524088331782593No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG777340.33800968328561376No Hit
GCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG755910.32869130585384554No Hit
GCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG753910.3278216486040305Illumina Small RNA Adapter 2 (100% over 21bp)
GCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT753730.3277433794515472No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT713870.31041111046273334No Hit
CCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG711090.3092022868854904No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA697570.30332340387674067No Hit
CCCCTTTATAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGG646400.28107322314022276No Hit
GCTAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCA636230.2766510160249132No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGGT634600.27594224536631395No Hit
GCAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA628190.2731549938806567No Hit
CCTTGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGC493320.21450965723937918No Hit
CCCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTTGGAATTCTCGGGT492870.21431398435817076No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG467960.2034824033117244No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT463150.2013908776259192No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGTGGAATTCTCGGGTGCCAA436120.18963745989466887No Hit
GCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG388610.16897875192531245No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT365240.15881680696122363No Hit
GCTTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTGGAATTCTC353780.15383367091978342No Hit
CCCTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT344520.14980715785313975No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTGC300010.13045293575850592No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCC297380.12930933647499915No Hit
GCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT292880.12735260766291528No Hit
GCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG278670.12117369290297939No Hit
CCATTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT267400.11627317430027159No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATGGAATTCTCGGGTG267360.1162557811552753No Hit
GCTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGGT263730.114677353246861No Hit
CCTGCTCTGCTAGGCGGACTACTTGCGTGCCTTGGAATTCTCGGGTGCCA258120.11223796466112979No Hit
CCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCTGGAATTCT257800.11209881950115938No Hit
CCAAATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCAA255890.11126829682758602No Hit
CCTCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAA249020.10828102417447133No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)