FastQCFastQC Report
Mon 3 Jul 2023
SRR5766410_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766410_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16316874
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length30-70
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG7765944.759453311951787No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT7093554.347370703481562No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT4820822.954499740575309No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT4698442.8794976292640366Illumina Paired End PCR Primer 2 (100% over 27bp)
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG2754081.6878723216223892Illumina Small RNA Adapter 2 (100% over 21bp)
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG2180191.3361566682441748No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG1243770.762259977002948No Hit
CCCTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTTGGAATTCT1201200.7361704208784109No Hit
CCGATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT1087070.6662244250951499No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCC904590.5543892782404277No Hit
CCCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT900560.5519194424128053No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT831220.5094235574779826No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG819690.5023572529885321No Hit
CCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCTGGAATTCT804260.4929007847949307No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT733800.449718493873275No Hit
CCCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG729870.44730994429447696No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT694020.42533882409093804No Hit
CCGAAAAGAAACCAACCGGGATTGCCTTAGTAACGTGGAATTCTCGGGTG679540.4164645752611683No Hit
CCGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTTGGAATTCTCGGGT580260.3556195874283273No Hit
CCGTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTTGGAATT567010.3474991594590974No Hit
CCAATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT558180.34208758368790493No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG532250.3261960593677441No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGC508080.3113831730268923No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC498120.30527906264398436No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCA448570.2749117263515058No Hit
CCCCCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCA418260.2563358643328373No Hit
CCAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGA372450.2282606337463904RNA PCR Primer, Index 1 (100% over 22bp)
CCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA313290.19200368894188924No Hit
CCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCATGGAATTCTCGGGTGC306610.18790976752042088No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTG306090.18759107902653413No Hit
GCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT295840.18130923852203554No Hit
CCTCTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAG295520.18111312252579753No Hit
CCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG282040.17285173618427158No Hit
CCTTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTTGGAATT278650.1707741323491252No Hit
CCAAATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCAA266530.16334623899161077No Hit
CCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCCTGGAATTC266000.16302142187284158No Hit
GCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG259370.15895814357578542No Hit
CCTCTACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTTGGAA255110.15634734937586697No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGTGGAATTCTCGGGTGCCAA243490.1492258872624744No Hit
CCGGAAAAGAAACCAACCGGGATTGCCTTAGTAACGTGGAATTCTCGGGT241480.14799403366110445No Hit
CCAAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAG222290.1362332025117066No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCC218280.1337756239338491No Hit
CCGTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG209580.12844372028612833No Hit
CCAACTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAA209410.1283395336631269No Hit
CCATTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT208280.12764699905141144No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAA207080.12691156406551893No Hit
CCGGTACAACGGGGTATTGTAAGCAGTAGAGTTGGAATTCTCGGGTGCCA206970.1268441491918121No Hit
CCAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA204550.1253610219702622No Hit
CCTTGTGAAACTCCGTCGTGCTGGGGATAGAGCATTGGAATTCTCGGGTG203710.12484621748013744No Hit
CCAAGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGA201650.12358372075435527RNA PCR Primer, Index 1 (100% over 22bp)
CCACTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAG198720.12178803366380105No Hit
CCCCTACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTTGGAA196920.12068488118496226No Hit
GCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT196790.12060520906149058No Hit
CCCCCCACTAATAGGGAACGTGAGCTGGGTTTATGGAATTCTCGGGTGCC193520.11860114872493346No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC191880.1175960542442137No Hit
CCTAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCA191130.11713640737803087No Hit
CCACTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTTGGAATTC190610.1168177188841441No Hit
CCTCAATATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGCCAA181070.11097101074629859No Hit
CCTTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGTGGAATT177270.10864213329097228No Hit
CCTACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTTGGAATT174620.1070180476971263No Hit
CCTGTACAACGGGGTATTGTAAGCAGTAGAGTTGGAATTCTCGGGTGCCA165950.10170452992405285No Hit
CCTGTGAAACTCCGTCGTGCTGGGGATAGAGCATTGGAATTCTCGGGTGC164810.10100586668745495No Hit
CCCTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTTGGAATT163930.10046654769780045No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)