FastQCFastQC Report
Mon 3 Jul 2023
SRR5766414_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766414_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17450041
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length30-70
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT11696256.702706314558229Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT11169056.400586680569977No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG9148545.242704014277101No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT6085403.4873270498332927No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG2497751.4313719950572035No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG2016911.1558196338908315No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT1412370.8093791871319959No Hit
CCGATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT1342710.7694595101524404No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG1207520.6919869128101189No Hit
CCCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT1178450.6753279261636119No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT1105070.6332764490352774No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT1030040.5902794153893393No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCC897580.5143712842852346No Hit
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG820420.4701536231347537Illumina Small RNA Adapter 2 (100% over 21bp)
CCCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG780560.4473112699276752No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG719710.41244029168756685No Hit
CCAATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT671120.3845950849055312No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGC594920.3409275657289287No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC566680.32474422266400405No Hit
CCGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTTGGAATTCTCGGGT532820.30534025679366594No Hit
CCGAAAAGAAACCAACCGGGATTGCCTTAGTAACGTGGAATTCTCGGGTG527190.3021139033426913No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA439320.25175872079612877No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCA411010.23553526321227555No Hit
GCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT374940.21486482467290477Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTG371530.21291067453652399No Hit
CCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG346250.19842360255772465No Hit
GCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT326270.18697377272637927No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGTGGAATTCTCGGGTGCCAA322670.18491074032433505No Hit
CCATTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT320500.1836671902375473No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGTGGTTCAGCAGGAAT301690.1728878459368663Illumina Paired End PCR Primer 2 (96% over 27bp)
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAA300730.17233770396298784No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATTGGAATTCTCGGGTGCCAA294500.1687675117783391No Hit
CCAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA271750.15573029312653192No Hit
CCGTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG252800.14487071978799362No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG252410.1446472246111055No Hit
CCTTGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGC250730.1436844761568182No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCC238330.13657847566088813No Hit
CCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCATGGAATTCTCGGGTGC234520.134395099702058No Hit
CCTAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCA218000.12492807323489956No Hit
GCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG213660.12244097306132404No Hit
CCCCCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCA211460.12118023103785257No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATGGAATTCTCGGGTG209340.119965334178871No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC198710.11387365794727933No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCA195180.11185074006416373No Hit
CCCTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTTGGAATTCT186160.10668169776793075No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG185640.10638370419874658No Hit
CCTTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGG185340.10621178483190956No Hit
CCGGTACAACGGGGTATTGTAAGCAGTAGAGTTGGAATTCTCGGGTGCCA184750.10587367674379676No Hit
CCGGAAAAGAAACCAACCGGGATTGCCTTAGTAACGTGGAATTCTCGGGT179110.10264159264726082No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)