FastQCFastQC Report
Mon 3 Jul 2023
SRR5766419_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766419_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18409268
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length31-70
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT10342705.618202744400266No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT10053685.461205736154202Illumina Paired End PCR Primer 2 (100% over 27bp)
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG8945374.859166589350538No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT5429772.9494763181241104No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG2383371.2946576691696812No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG1768410.9606085369608395No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT1253090.6806843161824794No Hit
CCGATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT1214820.6598958741868498No Hit
CCCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT1038700.5642266710441718No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT986660.5359583010036032No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCC981290.5330412920274722No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG902010.48997602729233997No Hit
CCCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG823270.44720409306877384No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT790290.4292892036771913No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG733000.3981690092186175No Hit
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG720810.39154734452233514Illumina Small RNA Adapter 2 (100% over 21bp)
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC692850.3763593424790166No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAA637720.3464124700667077No Hit
CCAATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT619110.3363034315106934No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGC595170.3232991121645901No Hit
CCGAAAAGAAACCAACCGGGATTGCCTTAGTAACGTGGAATTCTCGGGTG526560.28602984105614626No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCA514970.27973409915049313No Hit
CCGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTTGGAATTCTCGGGT493050.267827053199508No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTG429040.23305652348588765No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCA386170.20976934009543455No Hit
CCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG372580.2023871888876842No Hit
CCTTTGCTCGAATATATTAGCATGGAATTGGAATTCTCGGGTGCCAAGGA361470.19635218521453432RNA PCR Primer, Index 1 (100% over 22bp)
GCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT322500.17518349996317073No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATTGGAATTCTCGGGTGCCAA313120.17008824033633493No Hit
CCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCATGGAATTCTCGGGTGC291340.1582572430364966No Hit
CCATTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT288730.15683947889725977No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA287130.15597035145558205No Hit
CCCCCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCA279830.1520049575029273No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCC269480.1463827893645744No Hit
GCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT267070.1450736661555473Illumina Paired End PCR Primer 2 (100% over 27bp)
CCCTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTTGGAATTCT264120.14347121243495398No Hit
GCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG264030.1434223240163596No Hit
CCAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA263310.1430312166676046No Hit
CCGTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG254150.13805546206399952No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGTGGTTCAGCAGGAAT245130.1331557561115412Illumina Paired End PCR Primer 2 (96% over 27bp)
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC244960.13306341132086297No Hit
CCTTGTGAAACTCCGTCGTGCTGGGGATAGAGCATTGGAATTCTCGGGTG241730.13130886029797598No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGTGGAATTCTCGGGTGCCAA235520.12793555941496426No Hit
CCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCTGGAATTCT215180.1168867768126359No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG213040.11572431885939193No Hit
CCTAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCA204100.11086806927901749No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCCAAG203180.11036832100005281No Hit
CCATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCCAAGGAGAT191810.10419208411763031RNA PCR Primer, Index 1 (95% over 22bp)
CCGGTACAACGGGGTATTGTAAGCAGTAGAGTTGGAATTCTCGGGTGCCA190700.10358912695496637No Hit
CCTCTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAG185470.10074816662998226No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)