FastQCFastQC Report
Mon 3 Jul 2023
SRR5766421_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766421_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16522895
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length30-70
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG7084524.287698977691258No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT6605983.99807660824571No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT5406973.2724107972604077Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT4433382.6831738626917376No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG1877371.1362234039494894No Hit
CCCTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTTGGAATTCT1486510.8996667956795706No Hit
CCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCTGGAATTCT1181250.7149170892873192No Hit
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG1126160.6815754745158158Illumina Small RNA Adapter 2 (100% over 21bp)
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG1037670.6280194844789608No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG986120.5968203513972582No Hit
CCGATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT937870.5676184470094375No Hit
CCCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT877790.531256780364458No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT724600.4385430035111886No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCC712390.4311532573438251No Hit
CCGTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTTGGAATT700730.42409638262544186No Hit
CCGAAAAGAAACCAACCGGGATTGCCTTAGTAACGTGGAATTCTCGGGTG664870.4023931641519238No Hit
CCCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG634130.38378867625800445No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT585820.3545504586212041No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT585570.35439915341712214No Hit
CCGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTTGGAATTCTCGGGT530650.32116042618439444No Hit
CCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCCTGGAATTC506940.30681064062926017No Hit
CCTCTACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTTGGAA505880.30616910656395263No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGC492410.2980167821680159No Hit
CCAATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT487480.29503304354351945No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG427560.2587682122291523No Hit
CCTTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTTGGAATT392560.2375854836576762No Hit
CCACTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTTGGAATTC381630.23097042013521238No Hit
CCCCTACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTTGGAA367950.22269099936784686No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAA357450.21633618079640402No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCA336670.20375969223311047No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA329790.1995957730167746No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC315100.1907050792249179No Hit
CCTTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGATGGAAT312600.1891920271840982No Hit
CCTTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGTGGAATT309500.18731584265348172No Hit
GCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT259010.1567582436370866Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTTGGAATT256070.1549788944370826No Hit
CCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCATGGAATTCTCGGGTGC246140.14896905173094666No Hit
CCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG237380.14366731737991437No Hit
GCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG235660.1426263375758304No Hit
CCTTGGGCCAGCATCAGTTTTGGTGGCAGGATTGGAATTCTCGGGTGCCA228610.13835953082071878No Hit
CCAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA227220.137518273886023No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGTGGAATTCTCGGGTGCCAA222440.1346253183839757No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCA215800.1306066521635585No Hit
CCGGAAAAGAAACCAACCGGGATTGCCTTAGTAACGTGGAATTCTCGGGT215720.13055823449825227No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTGGAATTCTC215370.1303464072125375No Hit
CCTTGTGAAACTCCGTCGTGCTGGGGATAGAGCATTGGAATTCTCGGGTG208740.1263337932002836No Hit
GCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT207310.1254683274329347No Hit
CCCTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTTGGAATT207000.1252807089798731No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTG200820.12154044433496673No Hit
ACTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT199920.12099574560027163No Hit
CCGCTACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTTGGAA197600.11959163330639092No Hit
CCCCCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCA196610.1189924646982263No Hit
CCATTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT195130.11809673789006103No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCC194880.11794543268597907No Hit
GCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT189110.11445330857576715No Hit
CCTAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCA188150.11387229659209237No Hit
CCTTGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGC183090.11080987926147323No Hit
ACATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG181210.10967206412677681No Hit
CCGTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG180720.10937550592677615No Hit
TCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT174180.10541736178799177Illumina Paired End PCR Primer 2 (100% over 27bp)
CCCTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCTGGAATTCT173740.10515106462880748No Hit
ACTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT171940.1040616671594173Illumina Paired End PCR Primer 2 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)