FastQCFastQC Report
Mon 3 Jul 2023
SRR5766427_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766427_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15255470
Total Bases980.7 Mbp
Sequences flagged as poor quality0
Sequence length30-70
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT13453128.818554918334211No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG7126284.671294951909053No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT4591413.0096811176581255No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT4205822.7569258764233417Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG2313121.5162561363235614No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG2073501.3591846072261293No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT1278140.8378240722835809No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT1222910.8016206645878494No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG1060020.6948458487349128No Hit
CCCTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTTGGAATTCT1025450.6721851244176679No Hit
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG1017320.6668558884124842Illumina Small RNA Adapter 2 (100% over 21bp)
CCGATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT937530.6145533372619788No Hit
CCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCTGGAATTCT917660.6015285009245864No Hit
CCCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT800040.5244282870340933No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG788870.5171063231745727No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC677720.4442472110003821No Hit
CCCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG676770.44362448354590184No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCC608240.3987028914874468No Hit
CCGTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTTGGAATT600950.3939242776525404No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT595700.39048288908830736No Hit
CCAATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT491150.32195009396629537No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGC475470.3116718134544528No Hit
CCGAAAAGAAACCAACCGGGATTGCCTTAGTAACGTGGAATTCTCGGGTG450470.2952842488628669No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTG445890.29228204702968835No Hit
CCGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTTGGAATTCTCGGGT358350.2348993508557914No Hit
GCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT349680.22921614345542946No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCA347810.2279903536239788No Hit
CCATTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT342120.22426054392293387No Hit
GCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG309120.20262895866204056No Hit
CCTTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTTGGAATT260500.17075842304432443No Hit
ACTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT258200.16925076710189854No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC255750.1676447857719231No Hit
CCTTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGG250830.164419713060299No Hit
CCTCTACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTTGGAA248510.16289894706619987No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATTGGAATTCTCGGGTGCCAA243150.15938545321776385No Hit
CCAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGA236870.1552688969923575RNA PCR Primer, Index 1 (100% over 22bp)
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCC221930.1454756883924258No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG219730.14403358270836625No Hit
CCTTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGTGGAATT217390.14249970666259382No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG210360.1378915234994399No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA210040.13768176267266757No Hit
CCTACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTTGGAATT205650.1348041063303851No Hit
CCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG199450.13073999031167183No Hit
CCGTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG199040.13047123425236978No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAA198560.13015659301221136No Hit
CTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG182660.11973410193196277No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTGGAATTCTC167280.10965247219521916No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGTGGAATTCTCGGGTGCCAA159900.10481486312778301No Hit
CCAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA155960.10223218294814909No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)