FastQCFastQC Report
Mon 3 Jul 2023
SRR5766431_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766431_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15292747
Total Bases984.9 Mbp
Sequences flagged as poor quality0
Sequence length30-70
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG11832857.737556895435464No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT9076865.9354019261549285No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT7056084.614004272744459Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT7046884.6079883489866145No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG2825761.847777904126708No Hit
CCGATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT1539851.0069152389691662No Hit
CCCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT1383640.9047687769895101No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG1347100.8808750971947683No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT1201620.7857450332500759No Hit
CCCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG1155300.7554561649388433No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG1139280.7449806107431189No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT899790.5883769606598475No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC882600.5771363378992669No Hit
CCAATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT812140.531062208771256No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCC808170.5284662068887951No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG720740.471295314046587No Hit
GCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG589270.3853264557374813No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGC545740.3568619816962904No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTG531260.3473934408252487No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAA392170.2564418282732331No Hit
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG388860.25427740352992173Illumina Small RNA Adapter 2 (100% over 21bp)
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT363660.23779900367147905No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCA355660.23256776562117976No Hit
GCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT336120.2197904666833238No Hit
CCGTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG321780.21041347247816236No Hit
CCATTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT276820.18101391463548047No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC271260.17737820419052247No Hit
CCGAAAAGAAACCAACCGGGATTGCCTTAGTAACGTGGAATTCTCGGGTG264690.1730820499417142No Hit
CCGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTTGGAATTCTCGGGT254020.16610488619212754No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCA241280.15777413959702596No Hit
CCCTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTTGGAATTCT239820.15681943865284634No Hit
GCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT228390.14934530728848125No Hit
CCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG217490.14221774544494853No Hit
CCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCTGGAATTCT211830.13851664452436177No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATTGGAATTCTCGGGTGCCAA202730.13256611124214635No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGTGGTTCAGCAGGAAT198320.12968239126691888Illumina Paired End PCR Primer 2 (96% over 27bp)
GCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT197670.12925735317533207Illumina Paired End PCR Primer 2 (100% over 27bp)
CCCCCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCA191900.12548432273155374No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCC188070.12297986751497296No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG176720.11555804853111086No Hit
CCATTCCAGGGGGCATGTCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG171110.1118896428483385No Hit
CCATTCCAGGGGACATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG170780.11167385427876365No Hit
CCAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA169990.1111572695212966No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTA163020.10659955336997336No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)