FastQCFastQC Report
Mon 3 Jul 2023
SRR5766432_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766432_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15811344
Total Bases913.9 Mbp
Sequences flagged as poor quality0
Sequence length43-65
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT187343011.848644871682003Illumina Paired End PCR Primer 2 (100% over 27bp)
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG7542134.770075206762942No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT6743394.26490625970822No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT4918573.1107855220909744No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG2198001.390141154350952No Hit
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG1458650.9225338465850847Illumina Small RNA Adapter 2 (100% over 21bp)
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG1192890.7544519934548258No Hit
CCGATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT1099850.6956081658839375No Hit
CCCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT916670.5797546369239706No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCC852290.5390370356877947No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT795800.5033095225807497No Hit
CCCTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTTGGAATTCT792700.5013489049381255No Hit
CCCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG786240.4972632307538183No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT678100.42886929789143796No Hit
CCGAAAAGAAACCAACCGGGATTGCCTTAGTAACGTGGAATTCTCGGGTG666660.42163398633285065No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT662240.41883852504885105No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG652190.41248232914292426No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGC630040.3984733998577224No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC582750.36856449394814256No Hit
CCAATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT559340.35375866845980963No Hit
CCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCTGGAATTCT536180.33911095729749474No Hit
GCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT505660.31980836037720767Illumina Paired End PCR Primer 2 (100% over 27bp)
CCGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTTGGAATTCTCGGGT473800.29965827066946366No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCA428530.27102692851410987No Hit
CCAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGA389920.24660775200387774RNA PCR Primer, Index 1 (100% over 22bp)
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTG378390.2393155192879239No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG328310.20764205749998227No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGTGGTTCAGCAGGAAT327680.2072436093984167Illumina Paired End PCR Primer 2 (96% over 27bp)
ACTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT318060.20115937013324103Illumina Paired End PCR Primer 2 (100% over 27bp)
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAA296270.18737812547750526No Hit
CCGTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTTGGAATT295020.18658755384741488No Hit
CCCCCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCA278160.1759243237007556No Hit
CCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCATGGAATTCTCGGGTGC277160.1752918663966833No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCC261980.16569116452086552No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA249440.15776014992779866No Hit
CCAAGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGA233620.14775467537737463RNA PCR Primer, Index 1 (100% over 22bp)
CCGTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG232430.14700205118552856No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC223750.14151232178618087No Hit
CCTTGTGAAACTCCGTCGTGCTGGGGATAGAGCATTGGAATTCTCGGGTG207140.13100720596553966No Hit
CCGGAAAAGAAACCAACCGGGATTGCCTTAGTAACGTGGAATTCTCGGGT202460.12804730578248122No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGTGGAATTCTCGGGTGCCAA197840.12512535303766714No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATTGGAATTCTCGGGTGCCAA193710.12251330437184846No Hit
GCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT191200.12092583653862696No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCA189280.1197115185148081No Hit
CCAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA184990.11699827668033785No Hit
CCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCCTGGAATTC180440.11412059594680883No Hit
CCTCTACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTTGGAA175860.11122394149415762No Hit
CCATTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT172420.10904828836814885No Hit
CCTAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCA164890.1042858848684843No Hit
TCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT163200.10321703202460208Illumina Paired End PCR Primer 2 (100% over 27bp)
CCAACTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAA162050.10248970612491892No Hit
CCGGTACAACGGGGTATTGTAAGCAGTAGAGTTGGAATTCTCGGGTGCCA161640.10223039863024926No Hit
CCCCCCACTAATAGGGAACGTGAGCTGGGTTTATGGAATTCTCGGGTGCC160520.10152204644968829No Hit
CCATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAA160460.10148409901144394Illumina Paired End PCR Primer 2 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)