FastQCFastQC Report
Mon 3 Jul 2023
SRR5766441_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766441_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17372228
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length42-65
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT224592912.928272642979358Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT11576626.663866028007461No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG7120754.098927322390657No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT4931882.8389450103924494No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG2562331.4749576162596991No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG1954931.125319101268991No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT1308580.7532597430795865No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC1097940.6320087440712843No Hit
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG1095000.6303163877425509Illumina Small RNA Adapter 2 (100% over 21bp)
CCGATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT1052660.6059441540831723No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT1043810.600849816154842No Hit
CCCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT920290.529747825091865No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTG770860.44373122434266926No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCC734710.42292214907610004No Hit
CCCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG700480.40321828610584665No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT695800.4005243311335771No Hit
GCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT675860.38904624093121504Illumina Paired End PCR Primer 2 (100% over 27bp)
ACTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT640850.3688933854655833Illumina Paired End PCR Primer 2 (100% over 27bp)
CCGAAAAGAAACCAACCGGGATTGCCTTAGTAACGTGGAATTCTCGGGTG639250.3679723752186536No Hit
TCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT601760.34639195387028077Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCA574530.33071751073034505No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGC548880.3159525652092524No Hit
CCGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTTGGAATTCTCGGGT531020.305671788327899No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG521040.29992698691267466No Hit
CCAATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT517480.29787773911325593No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG497710.28649750624963016No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC447680.25769866709094535No Hit
CCAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGA412400.2373903911461443RNA PCR Primer, Index 1 (100% over 22bp)
CCCTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTTGGAATTCT392360.22585473780334914No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGTGGTTCAGCAGGAAT378100.21764623397758767Illumina Paired End PCR Primer 2 (96% over 27bp)
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCC372360.2143421097167272No Hit
ACTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT353060.20323242361313698No Hit
CCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCTGGAATTCT304970.17555030937885457No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATTGGAATTCTCGGGTGCCAA299040.17213681515117116No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA292600.1684297489072789No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG287980.16577033181926923No Hit
CTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG266320.15330215560145768Illumina Paired End PCR Primer 2 (100% over 28bp)
CCTTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGG256050.14739042107897732No Hit
CCATTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT248440.14300986609201768No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAA231990.13354072949077114No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG228560.13156631377391545No Hit
GCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT216770.12477961951685183No Hit
CCCCCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCA209890.12081927545505389No Hit
CCTTGTGAAACTCCGTCGTGCTGGGGATAGAGCATTGGAATTCTCGGGTG209420.12054872869501829No Hit
CCATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAA208140.11981192049747447Illumina Paired End PCR Primer 2 (100% over 26bp)
CCTGTACAACGGGGTATTGTAAGCAGTAGAGTTGGAATTCTCGGGTGCCA206800.11904057441567081No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGTGGAATTCTCGGGTGCCAA203130.11692800716177568No Hit
CCGTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG200460.11539107131221164No Hit
CCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG199020.11456216208997488No Hit
CCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCATGGAATTCTCGGGTGC197250.11354329450430883No Hit
CCGGTACAACGGGGTATTGTAAGCAGTAGAGTTGGAATTCTCGGGTGCCA195900.11276619210846187No Hit
ACATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG191730.11036580915240118No Hit
CCTCTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAG185870.10699260912302094No Hit
TCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT181780.10463827667930677No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATGGAATTCTCGGGTG181780.10463827667930677No Hit
CCTGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG180380.10383239271324324Illumina Paired End PCR Primer 2 (100% over 28bp)
CTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG179200.10315314765613254No Hit
CCAAGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGA174530.10046494899790633RNA PCR Primer, Index 1 (100% over 22bp)
CCTTTGCTCGAATATATTAGCATGGAATTGGAATTCTCGGGTGCCAAGGA174140.10024045275021719RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)