FastQCFastQC Report
Mon 3 Jul 2023
SRR5766444_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766444_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16758027
Total Bases960.9 Mbp
Sequences flagged as poor quality0
Sequence length43-65
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT13749348.20462933971881Illumina Paired End PCR Primer 2 (100% over 27bp)
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG8561205.108715960417059No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT5900163.5207963324083433No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT5059823.0193411193334394No Hit
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG4795072.8613571275425205Illumina Small RNA Adapter 2 (100% over 21bp)
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG2668971.5926516886504598No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCC1098800.6556857797161921No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG1079400.6441092379192371No Hit
CCGATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT1060950.6330995886329578No Hit
CCCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT975980.5823955290202122No Hit
CCCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG876000.5227345677387917No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGC798080.4762374472842179No Hit
CCCTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTTGGAATTCT709610.4234448363163516No Hit
CCAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGA702640.41928563547486825RNA PCR Primer, Index 1 (100% over 22bp)
CCGAAAAGAAACCAACCGGGATTGCCTTAGTAACGTGGAATTCTCGGGTG660010.39384707996949764No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT645800.3853675614677074No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC641790.3829746783436976No Hit
CCTCTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAG621740.3710102627236488No Hit
CCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCTGGAATTCT589820.3519626743649476No Hit
CCAATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT563530.33627467004319783No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG560610.3345322214840685No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT554990.3311786047367032No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCA542660.3238209366770921No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT515450.30758394171342485No Hit
CCGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTTGGAATTCTCGGGT469420.28011650774879404No Hit
CCAAGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGA446510.26644544730713227RNA PCR Primer, Index 1 (100% over 22bp)
GCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT412930.24640728887714525Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTG390590.23307636394188885No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG353410.2108899812609205No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCC335210.20002951421429263No Hit
CCCCTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAG329260.19647897691058738No Hit
CCACTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAG323840.19324470595494325No Hit
CCAACTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAA308320.1839834725173793No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA295280.176202126897158No Hit
CCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCATGGAATTCTCGGGTGC295140.17611858484295317No Hit
ACTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT280480.1673705383097903Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC270890.16164790759675945No Hit
CCAAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAG267770.15978611324590897No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGTGGTTCAGCAGGAAT253440.15123498726908605Illumina Paired End PCR Primer 2 (96% over 27bp)
CCAAATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCAA248800.14846616490115455No Hit
CCGTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG235150.14032081461618362No Hit
CCGTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTTGGAATT234190.13974795481592195No Hit
CCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG228700.1364719128331754No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAA227160.1355529502369223No Hit
CCCCCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCA225870.13478316988032063No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGTGGAATTCTCGGGTGCCAA224580.134013389523719No Hit
CCAAAATCCAACGTTGCCATCGTTGGGCCCCTGGAATTCTCGGGTGCCAA217820.12997950176354292No Hit
GCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG196280.11712595999517127No Hit
CCAAAACTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCC189190.11289515167865526No Hit
CCTTGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGC188070.1122268152450166No Hit
CCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCCTGGAATTC180670.10781102095133277No Hit
CCGGAAAAGAAACCAACCGGGATTGCCTTAGTAACGTGGAATTCTCGGGT171950.10260754443228907No Hit
CCAAATATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGCCAAG169110.10091283418984824No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)