FastQCFastQC Report
Mon 3 Jul 2023
SRR5766447_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766447_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16893922
Total Bases969.1 Mbp
Sequences flagged as poor quality0
Sequence length45-65
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT180625010.691715044025893Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT11247686.657826406443689No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG6370223.770717066173266No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC6008323.5564980115333786No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG3856492.2827677314953863No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT3616232.1405509034551007No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTG3468942.0533657015819062No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC2880491.7050451635801327No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG2491311.4746782896239252No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT1154560.6834173852584379No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT1014510.6005177483357624No Hit
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG994030.5883950452713111Illumina Small RNA Adapter 2 (100% over 21bp)
CCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG975590.5774798770824205No Hit
CCCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC797510.4720691855923094No Hit
CCGATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT769100.4552524866635468No Hit
CCCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG760080.4499132883412153No Hit
CCGATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTG738510.43714538281874393No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCA706930.41845226940197777No Hit
CCCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT622240.36832181420039706No Hit
CCCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTG607170.35940144627162357No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCC595020.3522095106157114No Hit
CCAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGA554450.32819495674243077RNA PCR Primer, Index 1 (100% over 22bp)
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT502040.2971719651600144No Hit
GCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT487000.288269355097058Illumina Paired End PCR Primer 2 (100% over 27bp)
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAA444450.263082782079851No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGC415240.24579254006263318No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCC415140.24573334717657627No Hit
CCAATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT412560.2442061707163085No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG407370.24113405992995587No Hit
CCAATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTG394570.23355737051467385No Hit
CCGAATACTGCCAGCTGGGACTGAGGACTGCGACGTGGAATTCTCGGGTG391260.23159808598619078No Hit
CCGAAAAGAAACCAACCGGGATTGCCTTAGTAACGTGGAATTCTCGGGTG353160.20904559639851542No Hit
ACTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT352990.2089449684922187Illumina Paired End PCR Primer 2 (100% over 27bp)
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG339020.20067572231007103No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGTGGTTCAGCAGGAAT323500.19148898639404158Illumina Paired End PCR Primer 2 (96% over 27bp)
CCTTTGCTCGAATATATTAGCATGGAATAATTGGAATTCTCGGGTGCCAA323120.1912640534270254No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTGC320670.1898138277186316No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCA250780.14844391965347065No Hit
CCGTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC246180.14572104689485366No Hit
CCATTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT228550.13528534108302384No Hit
CCAAATATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGCCAAG222160.1315029156639885No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAA219770.13008820568722881No Hit
CCCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCAA215480.1275488308753882No Hit
ACTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT214710.12709304565275012No Hit
CTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG214650.12705752992111602Illumina Paired End PCR Primer 2 (100% over 28bp)
CCTTGGGCCAGCATCAGTTTTGGTGGCAGGATATGGAATTCTCGGGTGCC209620.1240801277524544No Hit
CCTGTACAACGGGGTATTGTAAGCAGTAGAGTTGGAATTCTCGGGTGCCA204800.12122703064451226No Hit
CCCTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTTGGAATTCT204150.12084227688514249No Hit
CCGTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG199330.11798917977720033No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATGGAATTCTCGGGTG190970.11304065450284427No Hit
CCGGTACAACGGGGTATTGTAAGCAGTAGAGTTGGAATTCTCGGGTGCCA190660.11285715655606791No Hit
TCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT185330.10970217572923563Illumina Paired End PCR Primer 2 (100% over 27bp)
GCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC183750.10876692812953677No Hit
CCTTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGG177450.10503777630795266No Hit
CCGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTTGGAATTCTCGGGT173840.10290091312129888No Hit
GCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT172460.1020840512937138No Hit
CCTGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG170560.10095938645863288Illumina Paired End PCR Primer 2 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)