FastQCFastQC Report
Mon 3 Jul 2023
SRR5766450_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766450_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15261541
Total Bases902.1 Mbp
Sequences flagged as poor quality0
Sequence length33-65
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT350489522.965538014804665Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT6699414.389733644852771No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG3749582.4568816477969033No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT2171081.42258242467127No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG1492250.9777846155902605No Hit
CCGAAAAGAAACCAACCGGGATTGCCTTAGTAACGTGGAATTCTCGGGTG1392540.9124504530702371No Hit
GCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT1253440.8213063150044939Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG917380.6011057467918869No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCC887030.5812191573577007No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT831380.5447549497131384No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCA787290.5158653375828823No Hit
CCGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTTGGAATTCTCGGGT687690.4506032516637737No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGTGGTTCAGCAGGAAT637260.4175594063535262Illumina Paired End PCR Primer 2 (96% over 27bp)
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG630250.41296616114978163Illumina Small RNA Adapter 2 (100% over 21bp)
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT587750.38511838352365596No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT571160.3742479216220695No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG565200.3703426803361469No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG536030.3512292762572272No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA529830.3471667769329454No Hit
CCGATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT505390.33115266669335686No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGC483300.3166783747460365No Hit
CCTTGACCTCAAATCAGGTAGGAGTACCCGCTGAATGGAATTCTCGGGTG443760.2907701129263421No Hit
CCTTGACCTCAAATCAGGTAGGAGTACCCGCTGAACTTGGAATTCTCGGG411730.2697827172236408No Hit
CCTGGAATTCTCGGGTGCCAAGAGATCGGAAGAGCGGTTCAGCAGGAATG408660.2677711248162948Illumina Paired End PCR Primer 2 (100% over 28bp)
CCCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT405650.2657988469185386No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCC398970.2614218315175381No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC394090.2582242514042324No Hit
CCGGAAAAGAAACCAACCGGGATTGCCTTAGTAACGTGGAATTCTCGGGT390240.25570157037221863No Hit
CCCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG344620.22580943824742206No Hit
CCTTGACCTCAAATCAGGTAGGAGTACCCGCTGAACTGGAATTCTCGGGT336510.22049542703453082No Hit
CCATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAA326000.2136088354380465Illumina Paired End PCR Primer 2 (100% over 26bp)
CCTGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG309250.2026335348442205Illumina Paired End PCR Primer 2 (100% over 28bp)
CCGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG286420.18767436394529227Illumina Paired End PCR Primer 2 (100% over 28bp)
CCTGGAATTCTCGGGTGCCAGGAGATCGGAAGAGCGGTTCAGCAGGAATG284280.18627214643658854Illumina Paired End PCR Primer 2 (100% over 28bp)
CCTTGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGC272780.17873686543187217No Hit
CCTGGAATTCTCGGGTGTCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT270250.17707910361083457Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCTGGAATTCT266580.1746743661075903No Hit
ACTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT264180.17310178572399734Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTGGAATTCTCGGGTGCCAAGGAATCGGAAGAGCGGTTCAGCAGGAATG256800.16826610104444892Illumina Paired End Adapter 2 (96% over 27bp)
GCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT248950.16312245270644687No Hit
CCAATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT242430.15885027599768595No Hit
CCTGTACAACGGGGTATTGTAAGCAGTAGAGTTGGAATTCTCGGGTGCCA239340.15682557875380998No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAGAGCGGTTCAGCAGGAATG236310.15484019601952384Illumina Paired End PCR Primer 2 (95% over 22bp)
CCTTGACCTCAAATCAGGTAGGAGTACCCGCTGATGGAATTCTCGGGTGC235970.15461741379851485No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTG227690.14919201147511907No Hit
CCCCCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCA220380.14440219372342544No Hit
CTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG219380.14374695189692835Illumina Paired End PCR Primer 2 (100% over 28bp)
TCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT218900.14343243582020976Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTGGAATTCTCGGGTACCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT209960.1375745738913259Illumina Paired End PCR Primer 2 (100% over 27bp)
CCGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTGGAATTCTCGGGTG203130.1330992722163509No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATTGGAAGAGCGGTTCAGCAGGAAT201750.13219503849578493Illumina Paired End PCR Primer 2 (96% over 27bp)
CCTCAATATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGCCAA193940.12707759983084277No Hit
CCGGTACAACGGGGTATTGTAAGCAGTAGAGTTGGAATTCTCGGGTGCCA182810.1197847583019303No Hit
CCTGGAATTCTTGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT181000.11859877059597061Illumina Paired End PCR Primer 2 (100% over 27bp)
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG178680.1170786095584974No Hit
CCATGTACACGGAGTCGAGCTCAACCCGCAACGCGATGGAATTCTCGGGT177060.11601711779957215No Hit
CCTCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTTGGAATTCTCGGGT172850.11325854971001945No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGTAGGAAT171750.11253778370087268Illumina Paired End PCR Primer 2 (96% over 27bp)
CCCTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTTGGAATTCT170770.11189564671090554No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC163310.10700754268523735No Hit
CCTGAAAAGAAACCAACCGGGATTGCCTTAGTAACGTGGAATTCTCGGGT161030.10551359132082401No Hit
CCGAAAAGAAACCAACCGGGATTGCCTTAGTAACGGTGGAATTCTCGGGT156020.1022308297700737No Hit
CCATTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT155250.10172629356367092No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)