FastQCFastQC Report
Mon 3 Jul 2023
SRR5766451_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766451_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16372003
Total Bases949.8 Mbp
Sequences flagged as poor quality0
Sequence length40-65
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT229758214.033603585340169Illumina Paired End PCR Primer 2 (100% over 27bp)
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG10603336.47650137860346No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT6086423.7175781118535096No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT5386533.2900861305730276No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG2516521.5370874290702243No Hit
CCGATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT1398090.8539517125668741No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCC1173500.7167724071391876No Hit
CCCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT1141600.6972879250022126No Hit
CCCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG1094340.6684215730964623No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG928210.5669495662809249No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG816780.49888825454038827No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT814580.49754449715163135No Hit
CCCTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTTGGAATTCT722740.44144873415916186No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGC702780.4292571898502584No Hit
CCAATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT697480.4260199561409804No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT681650.4163510109300615No Hit
CCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCTGGAATTCT634150.3873380673091741No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC600680.36689463103567715No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT543010.3316698634858545No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCA490630.299676221657179No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG458690.2801673075676812No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGTGGTTCAGCAGGAAT453390.27693007385840324Illumina Paired End PCR Primer 2 (96% over 27bp)
CCGAAAAGAAACCAACCGGGATTGCCTTAGTAACGTGGAATTCTCGGGTG406520.24830193348975077No Hit
CCGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTTGGAATTCTCGGGT405440.24764227077163375No Hit
GCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG378770.2313522664270218No Hit
ACTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT352090.2150561540942791Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTG348910.21311381386871234No Hit
GCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT337990.20644389082997358Illumina Paired End PCR Primer 2 (100% over 27bp)
CCGTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG297920.181969182390206No Hit
CCGTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTTGGAATT286560.17503050787371588No Hit
CCTCTACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTTGGAA271900.1660761972740904No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA253270.15469701538657182No Hit
CCTGGAATTCTCGGGTGCCAAGAGATCGGAAGAGCGGTTCAGCAGGAATG253050.15456263964769612Illumina Paired End PCR Primer 2 (100% over 28bp)
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCC250000.15269970326782864No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAA246980.15085509085235324No Hit
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG227740.13910332168886116Illumina Small RNA Adapter 2 (100% over 21bp)
CCCCTACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTTGGAA220180.13448568266204203No Hit
CTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG206880.12636205844819354Illumina Paired End PCR Primer 2 (100% over 28bp)
CCTGGAATTCTCGGGTGTCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT202580.12373562355198688Illumina Paired End PCR Primer 2 (100% over 27bp)
CCATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAA200550.12249570196145212Illumina Paired End PCR Primer 2 (100% over 26bp)
CCTTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGATGGAAT198760.12140237208605448No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC193850.1184033499138743No Hit
CCTTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGTGGAATT190990.11665646530849036No Hit
CCTGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG189520.11575859105327552Illumina Paired End PCR Primer 2 (100% over 28bp)
CCTGGAATTCTCGGGTGCCAGGAGATCGGAAGAGCGGTTCAGCAGGAATG186000.1136085792312645Illumina Paired End PCR Primer 2 (100% over 28bp)
CCCCCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCA184700.11281454077427179No Hit
CCTTGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGC183910.11233200971194546No Hit
CCGGAAAAGAAACCAACCGGGATTGCCTTAGTAACGTGGAATTCTCGGGT177800.10860002896407972No Hit
CCATTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT167590.1023637730826216No Hit
CCTTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTTGGAATT164760.10063521244162976No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)