FastQCFastQC Report
Mon 3 Jul 2023
SRR5766453_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766453_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16903334
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length30-70
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT14374338.503843087996724Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT7489274.430646640479328No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG6513033.8531037723090606No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC5006412.9617884850408798No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT4647452.7494280122489445No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG3719652.20054221255996No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTG3566282.1098086330187877No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC2762471.6342752264139133No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG2324721.3753026473948868No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCA1225600.7250640613265998No Hit
CCGATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT1034830.6122046692090448No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT917820.5429816390068373No Hit
CCCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT841010.4975408993279078No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCC812430.48063299228424405No Hit
CCGATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTG802440.4747229156094295No Hit
CCGAATACTGCCAGCTGGGACTGAGGACTGCGACGTGGAATTCTCGGGTG767180.4538631254638878No Hit
CCCTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTTGGAATTCT766490.4534549219698315No Hit
CCGAAAAGAAACCAACCGGGATTGCCTTAGTAACGTGGAATTCTCGGGTG759000.44902384346188745No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT737590.43635770316080835No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCC737570.4363458711754734No Hit
CCCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTG656150.3881778588768346No Hit
CCCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC624990.36974362572496056No Hit
CCGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTTGGAATTCTCGGGT601600.35590611887572No Hit
CCAATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT558110.3301774667648406No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGC517900.3063892602488953No Hit
CCCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG510950.30227764534499524No Hit
CCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCTGGAATTCT495020.29285346902569637No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG458770.2714079956060739No Hit
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG443910.2626168305021956Illumina Small RNA Adapter 2 (100% over 21bp)
CCAATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTG427050.25264246686482084No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT422380.24987969828910672No Hit
CCAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGA390920.23126798535720822RNA PCR Primer, Index 1 (100% over 22bp)
CCTTGGGCCAGCATCAGTTTTGGTGGCAGGATATGGAATTCTCGGGTGCC381090.22545256456507337No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG369090.21835337336409494No Hit
CCGGTACAACGGGGTATTGTAAGCAGTAGAGTTGGAATTCTCGGGTGCCA363670.21514690533831962No Hit
CCGTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC349570.20680535567716998No Hit
CCGGAATACTGCCAGCTGGGACTGAGGACTGCGACGTGGAATTCTCGGGT344740.2039479312187761No Hit
CCTGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCTTGGAATTCTCGGG342980.20290671650929928No Hit
CCGTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTTGGAATT331120.1958903492056656No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAA330390.19545848174093938No Hit
CCTTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGTGGAATT318940.18868467013667245No Hit
CCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCAAG309730.18323604088992146No Hit
CCTGTACAACGGGGTATTGTAAGCAGTAGAGTTGGAATTCTCGGGTGCCA307020.18163280687703384No Hit
CCGTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG291530.17246893423510415No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG282280.16699664101768324No Hit
CCAATACTGCCAGCTGGGACTGAGGACTGCGACGTGGAATTCTCGGGTGC268090.15860184742252623No Hit
CCCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCAA261520.15471504023999053No Hit
CCGGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCTTGGAATTCTCGGG256620.15181620383292432No Hit
CCTTGAGATTAAGCCTTTGTTGTCTGATTTGTTGGAATTCTCGGGTGCCA250820.14838492808578474No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA250410.14814237238641798No Hit
CCTTAAGTGGGAGCTTCGGCGCCAGTGAAATACCACTATGGAATTCTCGG248880.1472372255082932No Hit
CCGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTGGAATTCTCGGGTG246070.14557483156873077No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGTGGTTCAGCAGGAAT246010.14553933561272586Illumina Paired End PCR Primer 2 (96% over 27bp)
CCGGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATGGAATTCTCGG235840.13952277106989663No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCA231300.13683691039885978No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATTGGAATTCTCGGGTGCCAA226440.13396173796246352No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATGGAATTCTCGGGTG203830.1205856785412866No Hit
CCTCAATATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGCCAA191460.1132675956116113No Hit
CCTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATGGAATTCTCGG190010.11240977667482639No Hit
CCATTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT188050.11125024211199991No Hit
CCTTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGG182540.10799053015221731No Hit
CCGAATACTGCCAGCTGGGACTGAGGACTGCGACGTTGGAATTCTCGGGT173430.10260106083214117No Hit
CCGGGGCCAGCATCAGTTTTGGTGGCAGGATATGGAATTCTCGGGTGCCA173140.10242949704478418No Hit
CCTTGTGAAACTCCGTCGTGCTGGGGATAGAGCATTGGAATTCTCGGGTG169160.10007493196312633No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)