FastQCFastQC Report
Mon 3 Jul 2023
SRR5766457_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766457_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14080293
Total Bases949.8 Mbp
Sequences flagged as poor quality0
Sequence length30-70
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT586030141.62058985562303Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT4517813.2086051050216073No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG4099712.9116652615112484No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT2843012.0191412209958983No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGTGGTTCAGCAGGAAT1103530.7837407928940116Illumina Paired End PCR Primer 2 (96% over 27bp)
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG1039850.738514461311281No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG686200.4873478130036073No Hit
CCGATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT663380.4711407639031375No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT539310.38302469984111837No Hit
CCCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT522800.37129909157430174No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG508030.36080925304608363No Hit
CCATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAA497130.3530679368675069Illumina Paired End PCR Primer 2 (100% over 26bp)
CCAGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT495370.35181796287903955Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG463490.32917638858793635Illumina Paired End PCR Primer 2 (100% over 28bp)
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT435520.3093117451462125No Hit
ACTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT399900.28401397612961604Illumina Paired End PCR Primer 2 (100% over 27bp)
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG381160.27070459400241176Illumina Small RNA Adapter 2 (100% over 21bp)
CCTGGAATTCTCGGGTGTCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT380770.2704276111299672Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT371660.2639575753146614No Hit
CCTGGAATTCTCGGGTACCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT359200.25510832764630675Illumina Paired End PCR Primer 2 (100% over 27bp)
CCAATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT344710.24481734861625395No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCC343640.2440574212482652No Hit
CCTGGATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG314600.22343285043855265Illumina Paired End PCR Primer 2 (100% over 28bp)
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGTAGGAAT293640.20854679657589512Illumina Paired End PCR Primer 2 (96% over 27bp)
CCTGGAATTCTCGGGTGCCAAGGAGATTGGAAGAGCGGTTCAGCAGGAAT293000.2080922605800888Illumina Paired End PCR Primer 2 (96% over 27bp)
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC282200.20042196565085682No Hit
CCTGGAATCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG268370.19059972686647927Illumina Paired End PCR Primer 2 (100% over 28bp)
CATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT267490.18997473987224556Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTGGAATTCTCGGGTGCCAAGAGATCGGAAGAGCGGTTCAGCAGGAATG259510.18430724417453528Illumina Paired End PCR Primer 2 (100% over 28bp)
CTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG252470.17930734822066557Illumina Paired End PCR Primer 2 (100% over 28bp)
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGC240160.17056463242632805No Hit
CCTGGAATTCTTGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT223980.1590733942823491Illumina Paired End PCR Primer 2 (100% over 27bp)
CCCTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTTGGAATTCT220250.15642430168179028No Hit
CCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCTGGAATTCT212600.15099117610691767No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG211150.14996136799141893No Hit
CCCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG206930.14696427126907088No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTG200080.14209931568895617No Hit
CCTGGAATTCTCGGGTGCCAGGAGATCGGAAGAGCGGTTCAGCAGGAATG190430.13524576512718875Illumina Paired End PCR Primer 2 (100% over 28bp)
CCTGGAATTCTCGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG188520.13388925926470424Illumina Paired End PCR Primer 2 (100% over 28bp)
CCGAAAAGAAACCAACCGGGATTGCCTTAGTAACGTGGAATTCTCGGGTG176410.12528858596905618No Hit
GTATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG175390.12456416922573983Illumina Paired End PCR Primer 2 (100% over 31bp)
TCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT173040.12289516986613844Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAAGCGGTTCAGCAGGAATG172270.12234830624618394RNA PCR Primer, Index 1 (95% over 22bp)
CCTTTGCTCGAATATATTAGCATGGAATAATTGGAATTCTCGGGTGCCAA168880.11994068589339724No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAGAGCGGTTCAGCAGGAATG160350.11388257332429091Illumina Paired End PCR Primer 2 (95% over 22bp)
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAA160100.11370502020092906No Hit
CCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG145700.10347796029528647No Hit
CCTGGAATTCTCGGGTGCCAAGGAGACGGAAGAGCGGTTCAGCAGGAATG143340.10180185881075059Illumina Paired End Adapter 2 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)