FastQCFastQC Report
Mon 3 Jul 2023
SRR5766458_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766458_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14590778
Total Bases974 Mbp
Sequences flagged as poor quality0
Sequence length30-70
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT516831735.421805471922056Illumina Paired End PCR Primer 2 (100% over 27bp)
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG5229533.584133759008601No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT4645973.184182502125658No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT3942512.702056052117303No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG1413430.9687146223457036No Hit
CCGATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT891170.6107762039830912No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG887320.6081375509928257No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGTGGTTCAGCAGGAAT868130.5949854079062816Illumina Paired End PCR Primer 2 (96% over 27bp)
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG759660.5206439300221003Illumina Small RNA Adapter 2 (100% over 21bp)
CCCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT725620.49731412540167497No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT597220.40931333476528803No Hit
CCATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAA582530.3992453315374958Illumina Paired End PCR Primer 2 (100% over 26bp)
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCC527690.36165994712550625No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT506660.34724673351893914No Hit
CCAATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT464590.31841345266167437No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT443830.30418528744663237No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGC441540.3026158029407342No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG432730.2965777424617111No Hit
CCCTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTTGGAATTCT428880.2939390894714456No Hit
CCAGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT416170.28522810778150415Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG414440.2840424273469173Illumina Paired End PCR Primer 2 (100% over 28bp)
CCGAAAAGAAACCAACCGGGATTGCCTTAGTAACGTGGAATTCTCGGGTG398790.27331647428259137No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC387350.26547590539723104No Hit
CCCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG363020.2488009892275792No Hit
CCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCTGGAATTCT342580.23479214062471515No Hit
CCTGGAATTCTCGGGTGTCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT331410.22713662013087993Illumina Paired End PCR Primer 2 (100% over 27bp)
CATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT323130.22146180279077649Illumina Paired End PCR Primer 2 (100% over 27bp)
CCGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTTGGAATTCTCGGGT306330.20994768065143612No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTG290260.19893387453362665No Hit
CCTGGAATTCTCGGGTACCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT288580.19778246231969263Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTGGATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG257390.17640594627647682Illumina Paired End PCR Primer 2 (100% over 28bp)
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCA256720.17594675211972932No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATTGGAAGAGCGGTTCAGCAGGAAT252410.1729928314994581Illumina Paired End PCR Primer 2 (96% over 27bp)
CTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG250080.17139593241703766Illumina Paired End PCR Primer 2 (100% over 28bp)
ACTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT228710.15674969491003152Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGTAGGAAT227030.1555982826960975Illumina Paired End PCR Primer 2 (96% over 27bp)
CCGTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG206730.14168538511106124No Hit
CCTGGAATCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG206610.14160314138149452Illumina Paired End PCR Primer 2 (100% over 28bp)
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCC190620.13064416441672952No Hit
CCTGGAATTCTTGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT190440.13052079882237946Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTGGAATTCTCGGGTGCCAAGAGATCGGAAGAGCGGTTCAGCAGGAATG185980.12746407354014982Illumina Paired End PCR Primer 2 (100% over 28bp)
CCAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGA165420.11337298120771902RNA PCR Primer, Index 1 (100% over 22bp)
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAA156720.10741031081413205No Hit
CCGTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTTGGAATT156440.10721840877847637No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA150130.10289375933209319No Hit
CCTGAAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT147170.10086508066944752Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTGGAATTCTCGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG146460.10037847193617778Illumina Paired End PCR Primer 2 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)