FastQCFastQC Report
Mon 3 Jul 2023
SRR5766461_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766461_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15510573
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length30-70
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT408606026.343707611575667Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT6485354.181244625843288No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG4247502.7384546012581223No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT2992541.9293548987519675No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT1280510.825572337011663No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAA1205460.7771859879064429No Hit
CCTTTGCTCGAATATATTAGCATGGAATTGGAATTCTCGGGTGCCAAGGA1059400.683017964584545RNA PCR Primer, Index 1 (100% over 22bp)
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG1014330.6539603662611304No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG1001830.6459013474228192No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCA926360.5972442152846319No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGTGGTTCAGCAGGAAT926360.5972442152846319Illumina Paired End PCR Primer 2 (96% over 27bp)
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT855230.5513851744871063No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATTGGAATTCTCGGGTGCCAA688170.4436779995168457No Hit
CCGATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT662330.42701839577428896No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT654900.42222811497679674No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG631300.4070126874100654No Hit
CCCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT525480.33878825753245867No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCTGGAATTCTCGGGTGC496530.32012356990293006No Hit
CCATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAA446850.28809380543194635Illumina Paired End PCR Primer 2 (100% over 26bp)
CCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCCAAG413690.26671484025767456No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAA374180.24124189351354075No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCC370460.23884352950725934No Hit
CCAGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT362600.23377601846172932Illumina Paired End PCR Primer 2 (100% over 27bp)
CCAATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT357840.23070714408810045No Hit
CCTGGAATTCTCGGGTACCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT338880.21848322431415013Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTGGAATTCTCGGGTGTCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT332380.2142925345182283Illumina Paired End PCR Primer 2 (100% over 27bp)
ACTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT288300.18587321048680794Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATGGAATTCTCGGGTG287180.18515112239889525No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC287110.1851059918934007No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGTAGGAAT281250.18132792386200045Illumina Paired End PCR Primer 2 (96% over 27bp)
CCTGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG277580.1789617959310723Illumina Paired End PCR Primer 2 (100% over 28bp)
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG271220.1748613671461396Illumina Small RNA Adapter 2 (100% over 21bp)
CCGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTTGGAATTCTCGGGT266290.1716828901163097No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGC253490.1634304548258791No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCTGGAATTCTCGGGTGCCAAGG244900.1578922970801917Illumina Small RNA Adapter 2 (100% over 21bp)
CCTGGAATTCTCGGGTGCCAAGGAGATTGGAAGAGCGGTTCAGCAGGAAT235880.15207690908646637Illumina Paired End PCR Primer 2 (96% over 27bp)
CCGAAAAGAAACCAACCGGGATTGCCTTAGTAACGTGGAATTCTCGGGTG226440.1459907380597738No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAAGG219930.14179360104878136Illumina Small RNA Adapter 2 (100% over 21bp)
CCATTCCAGGGGGCATGCCTGTTTGAGCTGGAATTCTCGGGTGCCAAGGA218550.1409038853690318RNA PCR Primer, Index 1 (100% over 22bp)
CCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG214820.13849907414767978No Hit
CTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG210970.13601689634547995Illumina Paired End PCR Primer 2 (100% over 28bp)
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAAG209500.13506915573009456No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG209080.1347983726971273No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTG206970.13343801031722038No Hit
CCGATTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCA206520.13314788563904117No Hit
CCATTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT202610.13062702454641745No Hit
CATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT200060.128982984703402Illumina Paired End PCR Primer 2 (100% over 27bp)
CCCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG196180.12648146525599022No Hit
CCAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA192230.1239348153030839No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA189760.12234235318063363No Hit
CCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCTGGAATTCTCGGGTGCC185430.11955070905504264No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTTGGAATTCTCGGGTGCCAAG175300.1130196801884753No Hit
CCTGGAATTCTTGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT173680.11197523134703019Illumina Paired End PCR Primer 2 (100% over 27bp)
CCGCCAAGGACGTTTTCATTAATCAAGAACGTGGAATTCTCGGGTGCCAA169820.1094866063297597No Hit
CCTGGAATTCTCGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG168480.10862267951029275Illumina Paired End PCR Primer 2 (100% over 28bp)
CCCATTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCA167270.10784256648674424No Hit
CCTGGATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG165530.10672075106445134Illumina Paired End PCR Primer 2 (100% over 28bp)
CCTGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA163910.1056763022230062No Hit
CCTTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGG162390.10469632553226757No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)