FastQCFastQC Report
Mon 3 Jul 2023
SRR5766463_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766463_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14384865
Total Bases942.6 Mbp
Sequences flagged as poor quality0
Sequence length30-70
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT279892519.45742973604549Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT8657256.018304655622419No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG7017794.8785928821716436No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT4920393.420532622308239No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG1790111.2444399026337751No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG1310230.9108392744735526No Hit
CCGATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT1038660.7220505719031773No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT1008030.7007573585153563No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT927850.6450182188014973No Hit
CCCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT896820.623446935372699No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAA838490.5828973716472139No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT816300.5674714361240095No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG745010.5179124030708665No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCA615170.42765086776970096No Hit
CCAATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT569350.3957979445757746No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGTGGTTCAGCAGGAAT560830.38987505270296247Illumina Paired End PCR Primer 2 (96% over 27bp)
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC542160.37689613354035645No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCC536080.37266946891750463No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGC422080.29341950723903215No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTG381760.26539004710854086No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATTGGAATTCTCGGGTGCCAA365890.2543576182327745No Hit
ACTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT364050.25307849604427984Illumina Paired End PCR Primer 2 (100% over 27bp)
CTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG360920.25090259797363407Illumina Paired End PCR Primer 2 (100% over 28bp)
CCTTTGCTCGAATATATTAGCATGGAATTGGAATTCTCGGGTGCCAAGGA358290.2490742874542097RNA PCR Primer, Index 1 (100% over 22bp)
CCCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG352890.24532034190101887No Hit
CCGAAAAGAAACCAACCGGGATTGCCTTAGTAACGTGGAATTCTCGGGTG336530.23394727722505565No Hit
CCCTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTTGGAATTCT328500.22836502115244042No Hit
CCGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTTGGAATTCTCGGGT321170.22326938765153512No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCCAAG314670.21875074948565731No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG304710.21182680546532762No Hit
CCATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAA286630.19925803961316288Illumina Paired End PCR Primer 2 (100% over 26bp)
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAA286200.1989591143191125No Hit
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG280730.1951565065087507Illumina Small RNA Adapter 2 (100% over 21bp)
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCA259160.18016157954906079No Hit
CCATTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT240180.16696715610469753No Hit
CCTGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG228180.15862505487538464Illumina Paired End PCR Primer 2 (100% over 28bp)
CCAGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT220230.15309841281096484Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGG207360.14415150924252676No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA204280.14201036992700314No Hit
CCTGGAATTCTCGGGTGTCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT199530.13870828819040013Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCATGGAATTCTCGGGTGC194510.13521850917613756No Hit
CCTGGAATTCTCGGGTACCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT194180.13498910139233145Illumina Paired End PCR Primer 2 (100% over 27bp)
CCGTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG189370.13164530914958186No Hit
CCTTGGGCCAGCATCAGTTTTGGTGGCAGGATTGGAATTCTCGGGTGCCA188040.130720726263333No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCC185610.12903145076439718No Hit
CCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCTGGAATTCT181320.1260491495749178No Hit
CCAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA180440.12543739548476818No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC178810.12430426006778653No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATGGAATTCTCGGGTG175800.12221178300943387No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGTGGAATTCTCGGGTGCCAA172380.11983428415907971No Hit
CCTGGAATTCTCGGGTGCCAAGAGATCGGAAGAGCGGTTCAGCAGGAATG161050.11195795024840345Illumina Paired End PCR Primer 2 (100% over 28bp)
CCTGGAATTCTCGGGTGCCAAGGAGATTGGAAGAGCGGTTCAGCAGGAAT154000.10705696577618211Illumina Paired End PCR Primer 2 (96% over 27bp)
CCTGGAATTCTCGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG152780.10620885215120196Illumina Paired End PCR Primer 2 (100% over 28bp)
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGTAGGAAT151580.10537464202827068Illumina Paired End PCR Primer 2 (96% over 27bp)
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG148300.10309446769225851No Hit
CCTGGATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG144360.10035547778863409Illumina Paired End PCR Primer 2 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)