FastQCFastQC Report
Mon 3 Jul 2023
SRR5766464_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766464_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14929283
Total Bases968.7 Mbp
Sequences flagged as poor quality0
Sequence length30-70
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT10940457.328181802166922Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT10177116.816877943836955No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG8188495.484851482820709No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT5541133.7115848095317103No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG2283161.5293165786997271No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG1937621.2978654098793625No Hit
CCGATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT1217870.8157592029034482No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT1127020.7549056441625495No Hit
CCCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT1036510.6942798257625634No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC999730.6696436794720818No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT970690.6501919750600214No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCC847310.5675490242900479No Hit
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG716680.4800498456623804Illumina Small RNA Adapter 2 (100% over 21bp)
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTG684690.4586221588806375No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT679410.455085485351172No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAA677990.454134334515596No Hit
CCAATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT661400.4430219455281275No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGC659280.44160191752008454No Hit
CCCTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTTGGAATTCT570250.3819674394276001No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG532320.35656099492520843No Hit
CCTTTGCTCGAATATATTAGCATGGAATTGGAATTCTCGGGTGCCAAGGA523270.35049908290974185RNA PCR Primer, Index 1 (100% over 22bp)
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCA521700.34944745839435154No Hit
CCCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG480530.3218707824079696No Hit
CCGAAAAGAAACCAACCGGGATTGCCTTAGTAACGTGGAATTCTCGGGTG457340.3063375515086692No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCA451660.3025329481663654No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATTGGAATTCTCGGGTGCCAA398930.2672131005889566No Hit
CCGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTTGGAATTCTCGGGT380480.2548548379717901No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCCAAG380370.25478115727325956No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC354270.23729873698556053No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCC344650.23085502498679944No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG336180.22518161119994845No Hit
CCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCTGGAATTCT327550.21940102548796214No Hit
CCATTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT259450.17378597485224173No Hit
CCGTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG252600.16919767680738587No Hit
CCTTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGG235080.15746235100506836No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGTGGTTCAGCAGGAAT230440.1543543651761441Illumina Paired End PCR Primer 2 (96% over 27bp)
CCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA224070.1500875829066942No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAA219640.14712026022951No Hit
CCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCATGGAATTCTCGGGTGC219480.14701308830437468No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG217280.14553947433376407No Hit
CCAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGA199590.13369027836099026RNA PCR Primer, Index 1 (100% over 22bp)
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG188460.1262351313187646No Hit
CCTTGGGCCAGCATCAGTTTTGGTGGCAGGATTGGAATTCTCGGGTGCCA184460.12355583319038162No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGTGGAATTCTCGGGTGCCAA183210.12271855252526194No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATGGAATTCTCGGGTG170120.1139505494001286No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAAGG169760.11370941256857413Illumina Small RNA Adapter 2 (100% over 21bp)
CCTTGTGAAACTCCGTCGTGCTGGGGATAGAGCATTGGAATTCTCGGGTG154110.10322665864127567No Hit
CCGATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTG149900.10040669736115257No Hit
CCAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA149340.10003159562317895No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)