FastQCFastQC Report
Mon 3 Jul 2023
SRR5766466_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766466_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15216665
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length30-70
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT181697311.940678197226527Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT7526244.946050925087724No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG5718943.7583399516254055No Hit
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG4401062.8922631864472277Illumina Small RNA Adapter 2 (100% over 21bp)
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT3196532.100677119460802No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG1806781.1873692428662916No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG1548381.0175554236095754No Hit
CCAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGA1470090.9661052536807506RNA PCR Primer, Index 1 (100% over 22bp)
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC1336920.8785893623865676No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT791590.5202125432872446No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTG762430.5010493429407824No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG724090.4758532832259894No Hit
CCGATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT699020.45937792545212763No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCC660570.4341095765727904No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT623340.4096429802456714No Hit
CCCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT601020.39497485158541634No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCA597120.3924118721152105No Hit
CCGAAAAGAAACCAACCGGGATTGCCTTAGTAACGTGGAATTCTCGGGTG553140.36350934978196603No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC550470.3617546946062097No Hit
CCGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTTGGAATTCTCGGGT504720.33168897389802565No Hit
CCAAGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGA495070.32534724264482395RNA PCR Primer, Index 1 (100% over 22bp)
CCCTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTTGGAATTCT475310.31236147999578096No Hit
CCAAATATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGCCAAG448400.29467692165136056No Hit
CCTCTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAG432520.28424099498806077No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGC408280.26831109181939666No Hit
CCAATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT386060.2537086805814546No Hit
CCTCTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGGTGCCAAGG373840.2456780115748096Illumina Small RNA Adapter 2 (100% over 21bp)
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGTGGTTCAGCAGGAAT372070.24451481319987Illumina Paired End PCR Primer 2 (96% over 27bp)
CCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCTGGAATTCT352050.23135818525281326No Hit
CCAAAATCCAACGTTGCCATCGTTGGGCCCCTGGAATTCTCGGGTGCCAA326970.21487625573672026No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT318650.2094085662002811No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCC310420.20400002234392361No Hit
CCCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG280670.18444908920581482No Hit
CCTAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG270960.17806792749922537Illumina Small RNA Adapter 2 (100% over 21bp)
CCAAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAG243290.15988391674522637No Hit
CCAGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGAG238420.1566834782785847RNA PCR Primer, Index 1 (95% over 22bp)
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAA232530.15281272210435073No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG228820.1503746057365395No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATTGGAATTCTCGGGTGCCAA223010.1465564235001559No Hit
CCCCTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAG222370.14613583199735292No Hit
CCGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG212770.1398269594553077Illumina Paired End PCR Primer 2 (100% over 28bp)
CCTGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCTTGGAATTCTCGGG197850.1300219200462125No Hit
CCCCTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGGTGCCAAGG194180.12761009064732645Illumina Small RNA Adapter 2 (100% over 21bp)
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG192900.12676890764172044No Hit
CCTTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG192120.1262563117476793No Hit
CCATTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT177600.11671414202783592No Hit
CCCCCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCA176040.11568895023975358No Hit
CCTGTACAACGGGGTATTGTAAGCAGTAGAGTTGGAATTCTCGGGTGCCA169470.11137131559379142No Hit
ACTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT169130.1111478763579273Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTGC168550.11076671530851209No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA166520.10943265163555876No Hit
CCGGTACAACGGGGTATTGTAAGCAGTAGAGTTGGAATTCTCGGGTGCCA166420.10936693421324581No Hit
CCGATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTG163160.10722454624584297No Hit
CCTTGGGCCAGCATCAGTTTTGGTGGCAGGATATGGAATTCTCGGGTGCC162170.10657394376494456No Hit
CCAAATCCAACGTTGCCATCGTTGGGCCCCTGGAATTCTCGGGTGCCAAG161650.1062322131689171No Hit
CCTGGAATTCTCGGGTGTCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT160680.10559475417248128Illumina Paired End PCR Primer 2 (100% over 27bp)
CCGGAAAAGAAACCAACCGGGATTGCCTTAGTAACGTGGAATTCTCGGGT155580.1022431656345198No Hit
CCGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTGGAATTCTCGGGTG155310.10206572859427476No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)