FastQCFastQC Report
Mon 3 Jul 2023
SRR5766467_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766467_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12627535
Total Bases828.6 Mbp
Sequences flagged as poor quality0
Sequence length30-70
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT146767611.622822664914413Illumina Paired End PCR Primer 2 (100% over 27bp)
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG7324435.800364045714385No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT5286954.186842483509252No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT3786422.9985424708781245No Hit
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG1865591.4773983996084747Illumina Small RNA Adapter 2 (100% over 21bp)
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG1710151.3543023242461811No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG809100.6407426310835804No Hit
CCGATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT803060.635959433096008No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG772330.6116237254539385No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCC725160.57426884977947No Hit
CCCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT683160.5410082015215163No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG682180.5402321197288307No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT541680.4289673321040092No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT494250.39140655717842004No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGC460920.3650118570251438No Hit
CCAATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT449170.355706794714883No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT431550.34175316085047475No Hit
CCGAAAAGAAACCAACCGGGATTGCCTTAGTAACGTGGAATTCTCGGGTG419800.3324480985402139No Hit
CCGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTTGGAATTCTCGGGT412610.32675419232653086No Hit
CCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCTGGAATTCT403660.31966650656680023No Hit
CCCTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTTGGAATTCT366080.28990614557789784No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC356380.2822245196707038No Hit
CCCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG336200.2662435701029536No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCA320940.25415886790256376No Hit
CCAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGA315520.24986666043689448RNA PCR Primer, Index 1 (100% over 22bp)
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGTGGTTCAGCAGGAAT308850.24458455272545276Illumina Paired End PCR Primer 2 (96% over 27bp)
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAA250030.19800380676038512No Hit
CCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG218030.17266236046861086No Hit
CCATGTACACGGAGTCGAGCTCAACCCGCAACGCGATGGAATTCTCGGGT200120.15847906974718343No Hit
CCAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA187470.1484612792599664No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTG186500.147693116669247No Hit
CCTCTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAG184790.1463389331330303No Hit
CCAAGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGA182950.1448817999712533RNA PCR Primer, Index 1 (100% over 22bp)
CCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA176880.14007484437778236No Hit
CCATTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT176240.13956801545194686No Hit
CCGGAAAAGAAACCAACCGGGATTGCCTTAGTAACGTGGAATTCTCGGGT166620.13194974316048225No Hit
CCGTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG162990.1290750728467591No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCC157120.12442650129261175No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATTGGAATTCTCGGGTGCCAA156040.12357122748026436No Hit
CCCCCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCA147330.11667360256772204No Hit
CCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCATGGAATTCTCGGGTGC146700.11617469284385273No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGTGGAATTCTCGGGTGCCAA144440.11438495319949617No Hit
CCTAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCA143490.11363262901270914No Hit
CCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCCTGGAATTC141810.11230220308239099No Hit
CCTGGAATTCTCGGGTGTCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT140900.11158155570346864Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTTGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGC138850.10995811930040186No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCA131640.10424837468278646No Hit
CCGTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTTGGAATT126840.10044715773902033No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)